Single-shot fluorescence spectra of individual micrometer-sized bioaerosols illuminated by a 351- or a 266-nm ultraviolet laser

Optics Letters

Volumn 24, Issue 2, 1999, Pages 116-118

  Pan, Yong Le ^a   Holler, Stephen ^a   Chang, Richard K ^a   Hill, Steven C ^b   Pinnick, Ronald G ^b   Niles, Stanley ^b   Bottiger, Jerold R ^c  

^a Yale University   (United States)

^b U S ARMY RESEARCH LABORATORY   (United States)

^c US Army Edgewood Res Devmt E   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0001720381     PISSN: 01469592     EISSN: None     Source Type: Journal    
DOI: 10.1364/OL.24.000116     Document Type: Article

References (9)

1. M. Seaver, J. D. Eversole, J. J. Hardgrove, W. K. Cary, and D. C. Roselle, "Size and fluorescence measurements for field detection of biological aerosols," Aerosol Sci. Technol. (to be published).
2. P. P. Hairston, J. Ho, and F. R. Quant. J. Aerosol Sci. 28, 471 (1997).
3. R. G. Pinnick, S. C. Hill, P. Nachman, J. D. Pendleton, G. I. Fernadez, M. W. Mayo, and J. G. Bruno, Aerosol Sci. Technol. 23, 653 (1995).
4. G. Chen, P. Nachman, R. G. Pinnick, S. C. Hill, and R. K. Chang, Opt. Lett. 21, 1307 (1996).
5. R. G. Pinnick, S. C. Hill, P. Nachman, G. Videen, G. Chen, and R. K. Chang, Aerosol Sci. Technol. 28, 95 (1998).
6. The problem of using fluorescence to classify atmospheric bioaerosols is challenging because (1) there are so many possible types of biological aerosol particle, (2) differences between the emission spectra of highly purified bacterial or protein samples may be small (spectra may be dominated by a small number of primary fluorophors, e.g., aromatic amino acids, reduced nicotinamide compounds, and flavins), (3) differences may depend on growth conditions, the atmospheric environment (temperature, humidity, sunlight, etc.), and other factors, and (4) naturally occurring biological aerosols may be mixtures of several types of particle and compound.
7. S. Holler, Y. L. Pan, R. K. Chang, S. C. Hill, D. B. Hillis, and J. R. Bottiger, Opt. Lett. 23, 1489 (1998).
8. G. W. Faris, R. A. Copeland, K. Mortelmans, and B. V. Bronk, Appl. Opt. 36, 958 (1997).
9. M. Seaver, D. C. Roselle, J. G. Pinto, and J. D. Eversole, Appl. Opt. 37, 5344 (1998).