Measurement of oxidant-induced signal transduction proteins using cell imaging

Free Radical Biology and Medicine

Volumn 27, Issue 11-12, 1999, Pages 1164-1172

  Poynter, Matthew E ^a   Janssen Heininger, Yvonne M W ^a   Buder Hoffmann, Sylke ^a   Taatjes, Douglas J ^a   Mossman, Brooke T ^a  

^a UNIVERSITY OF VERMONT   (United States)

Author keywords

Cell imaging; Cell signaling; Epithelial cells; Extracellular regulated kinase (ERK); Free radical; Mitogen activated protein kinase (MAPK); Nuclear factor B

Indexed keywords

FREE RADICAL; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; OXIDIZING AGENT; PEROXIDASE;

APOPTOSIS; CONFERENCE PAPER; CONFOCAL LASER MICROSCOPY; HUMAN; HUMAN CELL; IMAGING; INFLAMMATION; LIPID PEROXIDATION; MEASUREMENT; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; TECHNIQUE;

ANIMALS; IMMUNOHISTOCHEMISTRY; MAP KINASE SIGNALING SYSTEM; MITOGEN-ACTIVATED PROTEIN KINASES; NF-KAPPA B; OXIDANTS; PROTEINS; SIGNAL TRANSDUCTION;

EID: 0033405861     PISSN: 08915849     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0891-5849(99)00202-6     Document Type: Conference Paper

References (15)

1. Janssen Y.M.W., Borm P.J.A., Van Houten B., Mossman B.T. Cell and tissue responses to oxidative damage. Lab. Invest. 69:1993;261-274.
2. Halliwell B.H., Gutteridge J.M.C. Free radicals in biology and medicine. 1998;Oxford University Press, Oxford.
3. Beckman K.B., Ames B.N. The free radical theory of aging matures. Physiol. Rev. 78:1988;547-581.
4. Ghosh S., May M.J., Kopp E.B. NF-κB and rel proteins evolutionarily conserved mediators of immune responses . Ann. Rev. Immunol. 16:1998;225-260.
5. Baeuerle P.A., Henkel T. Function and activation of NF-κB in the immune system. Ann. Rev. Immunol. 12:1994;141-179.
6. Schreck R., Albermann K., Baeurele P.A. Nuclear factor-kappa B an oxidative stress-responsive transcription factor of eurkaryotic cells . Free Radic. Res. Commun. 365:1992;182-185.
7. Imbert V., Rupec R.A., Livolsi A., Pahl H.L., Traenckner B.M., Mueller-Dieckmann C., Farahifar D., Rossi B., Augberger P., Baeuerle P.A. Tyrosine phosphorylation of IκB activates NF-κB without proteolytic degradation of IκB-α Cell. 86:1996;787-798.
8. 2. Am. J. Physiol. 273:1997;L1029-L1035.
9. Janssen Y.M.W., Driscoll K.E., Howard B., Quinlan T.R., Treadwell M., Barchowsky A., Mossman B.T. Asbestos causes translocation of p65 protein and increases NF-κB DNA binding activity in rat lung epithelial and pleural mesothelial cells. Am. J. Pathol. 151:1997;389-401.
10. Malkinson A.M., Dwyer-Nield L.D., Rice P.L., Dinsdale D. Mouse lung epithelial cell lines - tools for the study of differentiation and the neoplastic phenotype. Toxicology. 123:1997;53-100.
11. BeruBe K.A., Quinlan T.R., Moulton G., Hemenway D., O'Shaughnessy P., Vacek P., Mossman B.T. Comparative proliferative and histopathologic changes in rat lungs after inhalation of chrysotile and crocidolite asbestos. Toxicol. Appl. Pharmacol. 137:1996;67-74.
12. Fung H., Kow Y.W., Van Houten B., Taatjes D.J., Hatahet Z., Janssen Y.M.W., Vacek P., Faux S.P., Mossman B.T. Asbestos increases mammalian AP-endonuclease gene expression, protein levels, and enzyme activity in mesothelial cells. Cancer Res. 58:1998;189-194.
13. Driscoll K.E., Carter J.M., Iype P.T., Kumari H.L., Crosby L.L., Aardema M.J., Isfort R.J., Cody D., Chestnut M.H., Burns J.L., LeBoeuf R.A. Establishment of immortalized alveolar type II epithelial cell lines from adult rats. In Vitro Cell. Dev. Biol. Anim. 31:1995;516-527.
14. Zanella C.L., Posada J., Tritton T.R., Mossmann B.T. Asbestos causes stimulation of the extracellular signal-regulated kinase 1 mitogen-activated protein kinase cascade after phosphorylation of the epidermal growth factor receptor. Cancer Res. 56:1996;5334-5338.
15. Robledo, R. F.; Buder-Hoffmann, S. A.; Cummings, A. B.; Walsh, E. S.; Taatjes, D. J.; Mossman, B. T. Increased phosphorylated ERK immunoreactivity associated with proliferative and morphologic alterations following chrysotile asbestos inhalation in mice. Am. J. Pathol. In revision.