Single-stranded oligodeoxyribonucleotides are substrates of Fpg protein from Escherichia coli

IUBMB Life

Volumn 48, Issue 6, 1999, Pages 613-618

  Ishchenko, Alexander A ^a   Bulychev, Nikolai V ^a   Maksakova, Galia A ^a   Johnson, Francis ^b   Nevinsky, Georgy A ^a  

^a INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

^b STONY BROOK UNIVERSITY   (United States)

Author keywords

8 Oxoguanine DNA glycosylase; Fpg protein; Single stranded oligodeoxyribonucleotide; Substrate specificity

Indexed keywords

BACTERIAL PROTEIN; OLIGODEOXYRIBONUCLEOTIDE; SINGLE STRANDED DNA;

ARTICLE; DNA REPAIR; ESCHERICHIA COLI; NONHUMAN; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN STRUCTURE; STRUCTURE ANALYSIS;

ESCHERICHIA COLI;

EID: 0033399115     PISSN: 15216543     EISSN: None     Source Type: Journal    
DOI: 10.1080/152165499306496     Document Type: Article

References (29)

1. Richter, C., Park, J. W., and Ames, B. N. (1988) Normal oxidative damage to mitochondrial and nuclear DNA is extensive. Proc. Natl Acad. Sci. U.S.A. 85, 6465-6467.
2. Cutler, R. G. (1991) Antioxidants and aging. Am. J. Clin. Nutr. 53, 373S-379S.
3. Ames, B. N. (1989) Endogenous oxidative DNA damage, aging, and cancer. Free Radic. Res. Commun. 7, 121-128.
4. Floyd, R. A., Watson, J. J., Harris, J., West, M., and Wong. P. K. (1986) Formation of 8-hydroxydeoxyguanosine, hydroxyl free radical adduct of DNA in granulocytes exposed to the tumor promoter, tetradecanoylphorbolacetate. Biochem. Biophys. Res. Commun. 137, 841-846.
5. Kasai, H., Okada, Y., Nishimura, S., Rao, M. S., and Reddy, J. K. (1989) Formation of 8-hydroxydeoxyguanosine in liver DNA of rats following long-term exposure to a peroxisome proliferator. Cancer Res. 49, 2603-2605.
6. Fraga, C. G., Shigenaga, M. K., Park, J. W., Degan, P., and Ames, B. N. (1990) Oxidative damage to DNA during aging: 8-Hydroxy-2′-deoxyguanosine in rat organ DNA and urine. Proc. Natl. Acad. Sci. U.S.A. 87, 4533-4537.
7. Boiteux, S., Gajewski, E., Laval, J., and Dizdaroglu, M. (1992) Substrate specificity of the Escherichia coli Fpg protein (formamidopyrimidine-DNA glycosylase): Excision of purine lesions in DNA produced by ionizing radiation or photosensitization. Biochemistry 31, 106-110.
8. Tchou, J., Kasai, H., Shibutani, S., Chung, M. H., Laval, J., and Grollman, A. P. (1991) 8-Oxoguanine (8-hydroxyguanine) DNA glycosylase and its substrate specificity. Proc. Natl Acad. Sci. U.S.A. 88, 4690-4694.
9. Bailly, V., Verly, W. G., O'Connor, T., and Laval, J. (1989) Mechanism of DNA strand nicking at apurinic/apyrimidinic sites by Escherichia coli [formamidopyrimidine]DNA glycosylase. Biochem. J. 262, 581-589.
10. Graves, R. J., Felzenszwalb, I., Laval, J., and O'Connor, T. R. (1992) Excision of 5′-terminal deoxyribose phosphate from damaged DNA is catalyzed by the Fpg protein of Escherichia coli. J Biol. Chem. 267, 14429-14435.
11. van der Kemp, P. A., Thomas, D., Barbey, R., de Oliveira, R., and Boiteux, S. (1996) Cloning and expression in Escherichia coli of the OGG1 gene of Saccharomyces cerevisiae, which codes for a DNA glycosylase that excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine. Proc. Natl. Acad. Sci. U.S.A. 93, 5197-5202.
12. Bjoras, M., Luna, L., Johnsen, B., Hoff, E., Haug, T., Rognes, T., and Seeberg, E. (1997) Opposite base-dependent reactions of a human base excision repair enzyme on DNA containing 7,8-dihydro-8-oxoguanine and abasic sites. EMBO J. 16, 6314-6322.
13. Lagravere, C., Malfoy, B., Leng, M., and Laval, J. (1984) Ring-opened alkylated guanine is not repaired in Z-DNA. Nature 310, 798-800.
14. Kouchakdjian, M., Bodepudi, V., Shibutani, S., Eisenberg, M., Johnson, P., Grollman, A. P., and Patel, D. J. (1991) NMR structural studies of the ionizing radiation adduct 7-hydro-8-oxodeoxyguanosine (8-oxo-7H-dG) opposite deoxyadenosine in a DNA duplex. 8-Oxo-7H-dG(syn).dA(anti) alignment at lesion site. Biochemistry 30, 1403-1412.
15. Tchou, J., Bodepudi, V., Shibutani, S., Antoshechkin, I., Miller, J., Grollman, A. P., and Johnson, F. J. (1994) Substrate specificity of Fpg protein. Recognition and cleavage of oxidatively damaged DNA. Biol. Chem. 269, 15318-15324.
16. Castaing, B., Boiteux, S., and Zelwer, C. (1992) DNA containing a chemically reduced apurinic site is a high-affinity ligand for the E. coli formamidopyrimidine-DNA glycosylase. Nucleic Acids Res. 20, 389-394.
17. Ishchenko, A. A., Bulychev, N. V., Zharkov, D. O., Maksakova, G. A., Johnson, F., and Nevinsky, G. A. (1997) Isolation of 8-oxoguanine-DNA-glycosylase from Escherichia coli and substrate specificity of the enzyme. Mol. Biol. (Mosk) 31, 332-337.
18. Bodepudi, V., Iden, C. R., and Johnson, F. (1991) An improved method for the preparation of the phosphoramidites of modified 2′-deoxynucleotides -incorporation of 8-oxo-2′-deoxy-7H-guanosine into synthetic oligomers. Nucleosides & Nucleotides 10, 755-761.
19. Bodepudi, V., Shibutani, S., and Johnson, F. (1992) Synthesis of 2′-deoxy-7,8-dihydro-8-oxoguanosine and 2′-deoxy-7.8-dihydro-8-oxoadenosine and their incorporation into oligomeric DNA. Chem. Res. Toxicol. 5, 608-617.
20. Fasman, G. D., ed. (1975) Handbook of Biochemistry and Molecular Biology, pp. 588-589. CRC Press, San Francisco.
21. Castaing, B., Geiger, A.. Seliger, H., Nehls, P., Laval, J., Zelwer, C., and Boiteux, S. (1993) Cleavage and binding of a DNA fragment containing a single 8-oxoguanine by wild type and mutant FPG proteins. Nucleic Acids Res. 21, 2899-2905.
22. Maxam, A. M., and Gilbert, W. A. (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 65, 499-560.
23. Cornish-Bowden, A. (1976) Principles of Enzyme Kinetics. Butterworths, London.
24. Nevinskii, G. A. (1995) An important role for weak interactions during the recognition of long DNA and RNA molecules by enzymes. Mol. Biol. (Mosk) 29, 16-37.
25. Vinogradova, N. L., Bulychev, N. V., Maksakova, G. A., Johnson, F., Nevinskii, and G. A. (1998) Uracil DNA glycosylase: Interpretation of X-ray data in the light of kinetic and thermodynamic studies. Mol. Biol. (Mosk) 32, 489-499.
26. Beloglazova, N. G., Lokhova, I. A., Maksakova. G. A., Tsvetkov, I. V., and Nevinskii, G. A. (1996) Apurine/apyrimidine endonuclease from human placenta. Recognition of apurinized DNA by the enzyme. Mol. Biol. (Mosk) 30, 220-230.
27. Bailly, V., and Verly, W. G. (1987) Escherichia coli endonuclease III is not an endonuclease but a beta-elimination catalyst. Biochem. J. 242, 565-572.
28. Mazumder, A., Gerlt, J. A., Absalon, M. J., Stubbe, J., Cunningham, R. P., Withka, J., and Bolton, P. H. (1991) Stereochemical studies of the beta-elimination reactions at aldehydic abasic sites in DNA: Endonuclease III from Escherichia coli, sodium hydroxide, and Lys-Trp-Lys. Biochemistry 30, 1119-1126.
29. Varshey, V., and Sande, J. H. (1991) Specificities and kinetics of uracil-excision from uracil containing DNA oligomers by E. coli uracil-DNA-glycosylase. Biochemistry 30, 4055-4061.