Tn5 mutagenesis of Pseudomonas stutzeri SF/PS, a bacterium associated with Alexandrium lusitanicum (Dinophyceae) and paralytic shellfish poisoning

Journal of Phycology

Volumn 35, Issue 6 SUPPL., 1999, Pages 1390-1396

  Plumley, F Gerald ^a   Wei, Zhengyu ^a   Toivanen, Tracie B ^a   Doucette, Gregory J ^b,c   Franca, Susana ^d  

^a UNIVERSITY OF ALASKA FAIRBANKS   (United States)

^b CENTER FOR COASTAL ENVIRONMENTAL HEALTH AND BIOMOLECULAR RESEARCH   (United States)

^c MEDICAL UNIVERSITY OF SOUTH CAROLINA   (United States)

^d NATIONAL INSTITUTE OF HEALTH DR RICARDO JORGE   (Portugal)

Author keywords

Alexandrium lusitanicum; Dinoflagellate; Harmful algal bloom; Paralytic shellfish poisoning; Pseudomonas stutzeri SF PS; Transconjugation

Indexed keywords

BACTERIAL GENE; BACTERIAL TOXIN; MUTAGENESIS; PLASMID; TRANSPOSON;

ALGAL BLOOM; BACTERIUM; POPULATION DYNAMICS; SHELLFISH; TOXIN;

ALEXANDRIUM LUSITANICUM; ALGAE; BACTERIA (MICROORGANISMS); DINOPHYCEAE; PSEUDOMONAS; PSEUDOMONAS STUTZERI;

EID: 0033392792     PISSN: 00223646     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1529-8817.1999.3561390.x     Document Type: Article

References (36)

1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. 1990. Basic local alignment search tool. J. Mol. Biol. 215:403-10.
2. Carmichael W. W., Evans, W. R., Yin, Q. Q., Bell, P. & Moczydlowski, E. 1997. Evidence for paralytic shellfish poisons in the freshwater cyanobacterium Lyngbya wollei comb. nov. Appl. Environ. Microbiol. 63:3104-10.
3. Carmichael W. W., Mahmood, N. A. & Hyde, E. G. 1990. Natural toxins from cyanobacteria (blue-green algae). In Hall, S. & Strichartz, G. [Eds.] Marine Toxins: Origin, Structure, and Molecular Pharmacology. American Chemical Society, Washington, DC, pp. 87-106.
4. Cembella, A. D. 1998. Ecophysiology and metabolism of paralytic shellfish toxins in marine microalgae. In Anderson, D. M., Cembella, A. D. & Hallegraeff, G. M. [Eds.] Physiological Ecology of Harmful Algal Blooms. Springer, Berlin, pp. 381-403.
5. de Lorenzo, V., Herrero, M., Jakubzik, U. & Timmis, K. N. 1990. Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in Gram-negative eubacteria. J. Bacteriol. 172:6568-72.
6. Doucette, G. J. 1995. Interactions between bacteria and harmful algae: a review. Nat. Toxins 3:65-74.
7. Doucette, G. J., Kodama, M., Franca, S. & Gallacher, S. 1997. Bacterial interactions with harmful algal bloom species: bloom ecology, toxigenesis, and cytology. In Anderson, D. M., Cembella, A. D. & Hallegraeff, G. M. [Eds.] Physiological Ecology of Harmful Algal Blooms. Springer, Berlin, pp. 619-47.
8. Doucette, G. J. & Powell, C. L. 1998. Algal-bacterial interactions: can they determine the PSP-related toxicity of dinoflagellates? In Reguera, B., Blanco, J., Fernández, M. L. & Wyatt, T. [Eds.] Harmful Algae. Xunta de Galicia Intergovernmental Oceanographic Commission, UNESCO, pp. 406-409.
9. Doucette, G. J. & Trick, C. G. 1995. Characterization of bacteria associated with different isolates of Alexandrium tamarense. In Lassus, P., Arzul, G., Erard, E., Gentien, P. & Marcaillou, C. [Eds.] Harmful Marine Algal Blooms. Lavoisier Science, Paris, pp. 33-8.
10. Focareta, T. & Manning, P. A. 1991. Distinguishing between the extracellular DNases of Vibrio cholerae and development of a transformation system. Mol. Microbiol. 5:2547-55.
11. Franca, S., Pinto, L., Alvito, P., Sousa, I., Vasconcelos, V. & Doucette, G. J. 1996. Studies on prokaryotes associated with PSP producing dinoflagellates. In Yasumoto, T., Oshima, Y. & Fukuyo, Y. [Eds.] Harmful and Toxic Algal Blooms. Intergovernmental Oceanographic Commission, UNESCO, pp. 347-50.
12. Franca, S., Viegas, S., Mascarenhas, V., Pinto, L. & Doucette, G. J. 1995. Prokaryotes in association with a toxic Alexandrium lusitanicum in culture. In Lassus, P., Arzul, G., Erard, E., Gentien, P. & Marcaillou, C. [Eds.] Harmful Marine Algal Blooms. Lavoisier Science, Paris, pp. 45-51.
13. Gallacher, S., Flynn, K. J., Franco, J. M., Brueggemann, E. E. & Hines, H. B. 1997. Evidence for production of paralytic shellfish toxins by bacteria associated with Alexandrium spp. (Dinophyta) in culture. Appl. Environ. Microbiol. 63:239-45.
14. Hall, S., Strichartz, G., Moczydlowski, E., Ravindran, A. & Reichardt, P. B. 1990. The saxitoxins: sources, chemistry and pharmacology. In Hall, S. & Strichartz, G. [Eds.] Marine Toxins: Origin, Structure, and Molecular Pharmacology. American Chemical Society, Washington, DC, pp. 29-65.
15. Haygood, M. G. & Nealson, K. H. 1985. Mechanisms of iron regulation of luminescence in Vibrio fischenri. J. Bacteriol. 162:209-16.
16. Jungnitz, H., Chhatwal, G. S. & Guzman, C. A. (unpublished). Novel two-component regulatory system involved in intracellular survival of Bordetella bronchiseptica. GenBank Accession Number AJ224803.
17. Kodama, M., Ogata, T., Sakamota, S., Sato, S., Honda, T. & Miwatani, T. 1990. Production of paralytic shellfish toxins by a bacterium Moraxella sp. isolated from Protogonyaulax tamarensis. Toxicon 28:707-14.
18. Kodama, M., Ogata, T. & Sato, S. 1988. Bacterial production of saxitoxin. Agric. Biol. Chem. 52:1075-7.
19. Koop, M., Doucette, G. J., Kodama, M., Gerdts, G., Schutt, C. & Medlin, L. 1997. Phylogenetic analysis of selected toxic and non-toxic bacterial strains isolated from the toxic dinoflagellate Alexandrium tamarense. FEMS Microbiol. Ecol. 24:251-7.
20. Kovach, M. E., Elzer, P. H., Hill, D. S., Robertson, G. T., Farris, M. A., Roop II, R. M. & Peterson, K. M. 1995. Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic resistance cassettes. Gene 166:175-6.
21. Kovach, M. E., Phillips, R. W., Elzer, P. H., Roop II, R. M. & Peterson, K. M. 1994. pBBR1MCS: a broad-host-range cloning vector. BioTechniques 16:800-2.
22. Levasseur, M., Monfort, P., Doucette, G. J. & Michaud, S. 1996. Preliminary study of the impact of bacteria as PSP producers in the Gulf of St. Lawrence, Canada. In Vasumoto, T., Oshima, Y. & Fukuyo, Y. [Eds.] Harmful and Toxic Algal Blooms. Intergovernmental Oceanographic Commission, UNESCO, pp. 363-6.
23. Oshima, Y. Kotaki, V., Harada, Y. & Yasumoto, T. 1984. Paralytic shellfish toxins in tropical waters. In Ragelis, E. [Ed.] Seafood Toxins. American Chemical Society, Washington, DC, pp. 160-70.
24. Parkinson, J. S. 1993. Signal transduction schemes of bacteria. Cell 73:857-71.
25. Plumley, F. G. 1997. Marine algal toxins: biochemistry, genetics and molecular biology. Limnol. Oceanogr. 42:1252-64.
26. Sambrook, J., Fritsch, E. F. & Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
27. Schleif, R. 1980. Assaying of organisms for the presence of restriction endonucleases. Methods Enzymol. 65:19-23.
28. Shah, G. R. & Caufield, P. W. 1993. Enhanced transformation of Streptococcus mutans by modifications in culture conditions. Anal. Biochem. 214:343-6.
29. Simon, R., Priefer, U. & Pühler, A. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. BioTechnology 1:784-91.
30. Soper, B. W. & Reddy, K. J. 1994. Identification of a nuclease and host restriction-modification in the unicellular, aerobic nitrogen-fixing cyanobacterium, Cyanothece sp. J. Bacteriol. 176: 5565-70.
31. Stewart, G. J. & Carlson, C. A. 1986. The biology of natural transformation. Ann. Rev. Microbiol. 40:211-35.
32. ++. J. Biol. Chem. 272:1440-3.
33. Wei, Z. 1998. Paralytic shellfish poison toxins: Tn5 transposon mutagenesis established for a bacterial producer, Pseudomonas stutzeri SF/PS, and its application to the study of antibiotic resistance. M.S. thesis, University of Alaska, Fairbanks, 64 pp.
34. Wright, G. D., Holman, T. R. & Walsh, C. T. 1993. Purification and characterization of VanR and the cytosolic domain of VanS: a two-component regulatory system required for vancomycin resistance in Enterococcus faecium BM4147. Biochemistry 32:5057-63.
35. Xu, P., Leong, S. & Sequoia, L. 1988. Molecular cloning of genes that specify virulence in Pseudomonas solanacearum. J. Bacteriol. 170:617-22.
36. Yoshinaga, I., Kawai, T. & Ishida, T. 1995. Lysis of Gymnodinium nagasakiense by marine bacteria. In Lassus, P., Arzul, G., Erard, E., Gentien, P. & Marcaillou, C. [Eds.] Harmful Marine Algal Blooms. Lavoisier Science, Paris, pp. 687-92.