Low prevalence of nosocomial Clostridium difficile transmission, as determined by comparison of arbitrarily primed PCR and epidemiological data

Journal of Hospital Infection

Volumn 43, Issue 4, 1999, Pages 265-273

  Wullt, M ^a,b   Laurell, M H ^a  

^a LUND UNIVERSITY   (Sweden)

^b LUND UNIVERSITY HOSPITAL   (Sweden)

Author keywords

AP PCR; Clostridium difficile; Epidemiological data; Nosocomial transmission

Indexed keywords

DNA;

ARTICLE; CLOSTRIDIUM DIFFICILE; DIARRHEA; DISEASE TRANSMISSION; ENZYME LINKED IMMUNOSORBENT ASSAY; FECES; HOSPITAL INFECTION; HUMAN; MAJOR CLINICAL STUDY; NONHUMAN; POLYMERASE CHAIN REACTION; PREVALENCE;

ADULT; AGED; CHILD; CLOSTRIDIUM DIFFICILE; CROSS INFECTION; DNA PRIMERS; DNA, BACTERIAL; ENTEROCOLITIS, PSEUDOMEMBRANOUS; HUMANS; POLYMERASE CHAIN REACTION; PREVALENCE; SWEDEN;

EID: 0033387812     PISSN: 01956701     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0195-6701(99)90422-X     Document Type: Article

References (29)

1. Bartlett JG. Antibiotic-associated diarrhea. Clin Infect Dis 1992; 15: 573-581.
2. Tabaqchali S, Jumaa P. Diagnosis and management of Clostridium difficile infection. Br Med J 1995; 310: 1375-1380.
3. McFarland LV, Mulligan ME, Kwok RY, Stamm WE. Nosocomial acquisition of Clostridium difficile infection. N Engl J Med 1989; 320: 204-210.
4. Fekety R, Kim KH, Brown D, Batts DH, Cudmore M, Silva J, Jr. Epidemiology of antibiotic-associated colitis; isolation of Clostridium difficile from the hospital environment. Am J Med 1981; 70: 906-908.
5. Clabots CR, Johnson S, Olson MM, Peterson LR, Gerding DN. Acquisition of Clostridium difficile by hospitalized patients: evidence for colonized new admissions as a source of infection. J Infect Dis 1992; 166: 561-567.
6. Cartmill TD, Panigrahi H, Worsley MA, McCann DC, Nice CN, Keith E. Management and control of a large outbreak of diarrhoea due to Clostridium difficile. J Hosp Infect 1994; 27: 1-15.
7. Barbut F, Mario N, Meyohas MC, Binet D, Frottier J, Petit JC. Investigation of a nosocomial outbreak of Clostridium difficile-associated diarrhea among AIDS patients by random amplified polymorphic DNA (RAPD) assay. J Hosp Infect 1994; 26: 181-189.
8. Simor AE, Yake SL, Tsimidis K. Infection due to Clostridium difficile among elderly residents of a long-term-care facility. Clin Infect Dis 1993; 17: 672-678.
9. Welsh J, McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Research 1990; 18: 7213-7218.
10. Williams JG, Kubelik AR, Kenneth JL, Rafalski JA, Tingey SV. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 1990; 18: 6531-6535.
11. Van Belkum A. DNA fingerprinting of medically important microorganisms by use of PCR. Clin Microbiol Rev 1994; 7: 174-184.
12. Killgore GE, Kato H. Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing. J Clin Microbiol 1994; 32: 1591-1593.
13. Willey S, Barlett J. Cultures for Clostridium difficile in stools containing a cytotoxin neutralized by Clostridium sordelli antitoxin. J Clin Microbiol 1979; 10: 880-884.
14. Wilks M, Tabaqchali S. Typing of Clostridium difficile by polymerase chain reaction with an arbitrary primer. J Hosp Infect 1994; 28: 231-234.
15. Gürtler V. Typing of Clostridium difficile strains by PCR-amplification of variable length 16S-23S rDNA spacer regions. J Gen Microbiol 1993; 139: 3089-3097.
16. Maslow JN, Mulligan ME, Arbeit RD. Molecular epidemiology: the application of contempary techniques to typing bacteria. Clin Infect Dis 1993; 17: 153-164.
17. Tang YJ, Houston ST, Gumerlock PH, et al. Comparison of arbitrarily primed PCR with restriction endonuclease and immunoblot analyses for typing Clostridium difficile isolates. J Clin Microbiol 1995; 33: 3169-3173.
18. Collier MC, Stock F, DeGirolami PC, Samore MH, Cartwright CP. Comparison of PCR-based approaches to molecular epidemiologic analysis of Clostridium difficile. J Clin Microbiol 1996; 34: 1153-1157.
19. Lemann F, Chambon C, Barbut F, et al. Arbitrary primed PCR rules out Clostridium difficile cross-infection among patients in a haematology unit. J Hosp Infect 1997; 35: 107-115.
20. Poxton IR, Aronsson B, Mollby R, Nord CE, Collee JG. Immunochemical fingerprinting of Clostridium difficile strains isolated from an outbreak of antibiotic-associated colitis with diarrhoea. J Med Microbiol 1984; 17: 317-324.
21. Testore GP, Pantosti A, Cerquetti M, Babudieri S, Panichi G, Gianfrilli PM. Evidence for cross-infection in an outbreak of Clostridium difficile-associated diarrhoea in a surgical unit. J Med Microbiol 1988; 1988: 125-128.
22. Samore MH, DeGirrolami PC, Tlucko A, Lichtenberg DA, Melvin ZA, Karchmer AW. Clostridium difficile colonization and diarrhea at a Tertiary Care Hospital. Clin Infect Dis 1994; 18: 1881-1887.
23. Cohen SH, Tang YJ, Muenzer J, Gumerlock PH, Silva J, Jr. Isolation of various genotypes of Clostridium difficile from patients and the environment in an oncology ward. Clin Infect Dis 1997; 24: 889-893.
24. Samore M, Killgore G, Johnson S, et al. Multicenter typing comparison of sporadic and outbreak Clostridium difficile isolates from geographically diverse hospitals. J Infect Dis 1997; 176: 1233-1238.
25. Nolan NPM, Kelly CP, Humphreys JFH. An epidemic of pseudomembranous colitis: importance of person to person spread. Gut 1987; 28: 1467-1473.
26. Van Dijck P, Avesani V, Delmée M. Genotyping of outbreak-related and sporadic isolates of Clostridium difficile belonging to serogroup C. J Clin Mirobiol 1996; 34: 3049-3055.
27. Kristjánsson MH, Samore MH, Gerding DN, et al. Comparison of restriction endonuclease analysis, ribotyping, and pulsed-field gel electrophoresis for molecular differentiation of Clostridium difficile strains. J Clin Microbiol 1994; 32: 1963-1969.
28. Samore MH, Bettin KM, DeGirolami PC, Clabots CR, Gerding DN, Karchmer AW. Wide diversity of Clostridium difficile types at a tertiary referral hospital. J Infect Dis 1994; 170: 615-621.
29. McFarland LV, Surawicz CM, Stamm WE. Risk factors for Clostridium difficile carriage and C. difficile-associated diarrhea in a cohort of hospitalized patients. J Infect Dis 1990; 162: 678-684.