Comparison of PCR-based approaches to molecular epidemiologic analysis of Clostridium difficile

Journal of Clinical Microbiology

Volumn 34, Issue 5, 1996, Pages 1153-1157

  Collier, Megan C ^a   Stock, Frida ^a,c   Degirolami, Paula C ^b   Samore, Matthew H ^b   Cartwright, Charles P ^a,d  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c NATIONAL INSTITUTES OF HEALTH   (United States)

^d Ortho Clinical Diagnostics Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIUM ISOLATION; CLOSTRIDIUM DIFFICILE; GENE CLUSTER; GENETIC ANALYSIS; GENOTYPE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; RIBOTYPING;

EID: 0029883853     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/jcm.34.5.1153-1157.1996     Document Type: Article

References (19)

1. Barbut, F., N. Mario, J. Frottier, and J. C. Petit. 1993. Use of the arbitrary primer PCR for investigating an outbreak of Clostridium difficile-associated diarrhea in AIDS patients. Eur. J. Clin Microbiol. Infect. Dis. 12:794-795
2. Bartlett, J. G. 1992. Antibiotic-associated diarrhea. Clin Infect. Dis. 15: 573-581.
3. Cartwright, C. P., F. Stock, S. E. Beekmann, E. C. Williams, and V. J. Gill. 1995. PCR amplification of rRNA intergenic spacer regions as a method for epidemiologic typing of Clostridium difficile J. Clin Microbiol 33:184-187
4. Clabots, C. R., L. R, Peterson, and D. N. Gerding. 1988 Characterization of a nosocomial Clostridium difficile outbreak by using plasmid profile typing and clindamycin susceptibility testing J Infect Dis. 158:731-736.
5. Gürtler, V. 1993. Typing of Clostridium difficile strains by PCR-amplification of variable length 16S-23S rDNA spacer regions. J Gen. Microbiol 139: 3089-3097.
6. Kato, H., N. Kato, K. Watanabe, K. Ueno, H. Ushijima, S. Hashira, and T. Abe. 1994. Application of typing by pulsed-field gel electrophoresis to the study of Clostridium difficile in a neonatal intensive care unit. J. Clin. Microbiol 32:2067-2070.
7. Killgore, G. E., and H. Kato. 1994. Use of arbitrary primer PCR to type Clostridium difficile and comparison of results with those by immunoblot typing. J. Clin. Microbiol. 32:1591-1593.
8. Kostman, J. R., T. D. Edlind, J. J. LiPuma, and T. L. Stull. 1992. Molecular epidemiology of Pseudomonas cepcia determined by polymerase chain re-action ribotyping. J. Clin Microbiol. 30:2084-2087.
9. Kristjánsson, M., M. H. Samore, D. N. Gerding, P. C. DeGirolami, K. M. Bettin, A. W. Karchmer, and R. D. Arbeit. 1994. Comparison of restriction endonuclease analysis, ribotyping, and pulsed-field gel electrophoresis for molecular differentiation of Clostridium difficile strains. J. Clin. Microbiol. 32:1963-1969.
10. Lyerly, D. M., H. C. Krivan, and T. D. Wilkins. 1988. Clostridium difficile: its disease and toxins. Clin. Microbiol Rev. 1:1-18.
11. Maslow, J. N., M. E. Mulligan, and R. D. Arbeit. 1993 Molecular epidemiology: application of contemporary techniques to the typing of microorganisms. Clin. Infect. Dis. 17:153-164.
12. McFarland, L. V., M. E. Mulligan, R. Y. Y. Kwok, and W. E. Stamm. 1989. Nosocomial acquisition of Clostridium difficile infection. N. Engl J. Med. 320:204-210.
13. McMullin, D. E., and L. L. Muldrow. 1992. Typing of toxigenic strains of Clostridium difficile using DNA fingerprints generated with arbitrary polymerase chain reaction primers. FEMS Microbiol Lett 79:5-10
14. Prévost, G., B. Jaulhac, and Y. Piemont. 1992. DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J. Clin. Microbiol. 30:967-973.
15. Saulnier, P., C. Bourneix, G. Prévost, and A. Andremont. 1993. Random amplified polymorphic DNA assay is less discriminant than pulsed-field gel electrophoresis for typing strains of methicillin-resistant Staphylococcus aureus, J. Clin. Microbiol. 31:982-985.
16. Silva, J., Jr., Y. J. Tang, and P. H. Gumerlock. 1994 Genotyping of Clostridium difficile isolates. J. Infect. Dis. 169:661-664.
17. Tenover, F. C., R. D. Arbeit, R. V. Goering, P. A. Mickelsen, B. E. Murray, D. H. Persing, and B. Swaminathan. 1995. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis criteria for bacterial strain typing J. Clin. Microbiol. 33:2233-2239.
18. Toma, S., G. Lesiak, M. Magus, H. L. Lo, and M. Delmee. 1988. Serotyping of Clostridium difficile J. Clin. Microbiol. 26:426-428.
19. van Belkum, A. 1994. DNA fingerprinting of medically important microorganisms by use of PCR. Clin Microbiol. Rev. 7:174-184.