Importance of assay conditions in visualization and quantitation of serum alkaline phosphatase isoenzymes separated by electrophoresis

Scandinavian Journal of Clinical and Laboratory Investigation

Volumn 59, Issue 8, 1999, Pages 593-605

  Hipolito Reis C ^a   Dias, P O ^a   Martins, Maria João ^a  

^a University of Porto   (Portugal)

Author keywords

Cellulose acetate; Methods comparison

Indexed keywords

ALKALINE PHOSPHATASE;

ALKALINE PHOSPHATASE BLOOD LEVEL; ANIMAL EXPERIMENT; ARTICLE; DIAGNOSTIC VALUE; ELECTROPHORESIS; ENZYME ACTIVITY; MALE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE DIAGNOSIS; RAT;

ANIMALIA; RATTUS NORVEGICUS;

EID: 0033366909     PISSN: 00365513     EISSN: None     Source Type: Journal    
DOI: 10.1080/00365519950185085     Document Type: Article

References (57)

1. 1 Epstein E, Kiechle FL, Artiss JD, Zak B. The clinical use of alkaline phosphatase enzymes. Clin Lab Med 1986; 6: 491-505.
2. 2 Moss DW, Henderson AR. Enzymes In: Burtis CA, Ashwood ER, editors. Tietz textbook of clinical chemistry. 2nd ed. Philadelphia: WB Saunders, 1994: 830-43.
3. 3 Balistreri WF, Rej R. Liver function. In: Burtis CA, Ashwood ER, editors. Tietz textbook of clinical chemistry. 2nd ed. Philadelphia: WB Saunders, 1994: 1449-512.
4. 4 Endres DB, Rude RK. Mineral and bone metabolism. In: Burtis CA, Ashwood ER, editors. Tietz textbook of clinical chemistry. 2nd ed. Philadelphia: WB Saunders, 1994: 1887-973.
5. 5 Moss DW. Alkaline phosphatase isoenzymes. Clin Chem 1982; 28: 2007-16.
6. 6 Van Hoof VO, De Broe ME. Interpretation and clinical significance of alkaline phosphatase isoenzyme patterns. Crit Rev Clin Lab Sci 1994; 31: 197-293.
7. 7 Price CP. Multiple forms of human serum alkaline phosphatase: detection and quantitation. Ann Clin Biochem 1993; 30: 355-72.
8. 8 Panteghini M. Benign inherited hyperphosphatasemia of intestinal origin: report of two cases and a brief review of the literature. Clin Chem 1991; 37: 1449-52.
9. 9 McComb RB, Bowers Jr GN. Study of optimum buffer conditions for measuring alkaline phosphatase activity in human serum. Clin Chem 1972; 18: 97-104.
10. 10 Morton RK. Transphosphorylation by phosphatases. Methods Enzymol 1955; II: 556-61.
11. 11 Van Belle H. Alkaline phosphatase. I. Kinetics and inhibition by levamisole of purified isoenzymes from humans. Clin Chem 1976; 22: 972-6.
12. 12 Van Belle H. Alkaline phosphatase. II. Conditions affecting determination of total activity in serum. Clin Chem 1976; 22: 977-81.
13. 13 Bretaudière J-P, Vassault A, Amsellem L, Pourci M-L, Thieu-Phung H, Bailly M. Criteria for establishing a standardized method for determining alkaline phosphatase activity in human serum. Clin Chem 1977; 23: 2263-74.
14. 14 Bowers GN, Kelley M-L, McComb RB. Continuous spectrophotometric measurement of serum alkaline phosphatase activity. Relative molecular activity and sensitivity of nine self-indicating phenolic substrates in 2-amino-2-methyl-1-propanol buffer. Clin Chem 1968; 14: 608-10.
15. 15 Babson AL, Greeley SJ, Coleman CM, Phillips GE. Phenolphthalein monophosphate as a substrate for serum alkaline phosphatase. Clin Chem 1966; 12: 482-90.
16. 16 Haije WG. Influence of buffer conditions on the activities of some iso-enzymes of alkaline phosphatase in the serum. Clin Chim Acta 1973; 48: 23-6.
17. 17 Moss DW. Kinetics of phosphatase action on naphthyl phosphates, determined by a highly sensitive spectrofluorimetric technique. Biochem J 1960; 76: 32P.
18. 18 Babson AL, Read PA. A new assay for prostatic acid phosphatase in serum. Am J Clin Pathol 1959; 32: 88-91.
19. 19 Seligman AM, Chauncey HH, Nachlas MM, Manheimer LH, Ravin HA. The colorimetric determination of phosphatases in human serum. J Biol Chem 1951; 190: 7-15.
20. 20 Stevens J, Thomas F. Alkaline phosphatase: reaction rate analysis at 340 nm. Clin Chim Acta 1972; 37: 541-3.
21. 21 Kind PRN, King EJ. Estimation of plasma phosphatase by determination of hydrolysed phenol with amino-antipyrine. J Clin Pathol 1954; 7: 322-6.
22. 22 McComb RB, Bowers Jr GN, Posen S. Alkaline phosphatase. New York: Plenum Press, 1979.
23. 23 Crofton PM. Biochemistry of alkaline phosphatase isoenzymes. Crit Rev Clin Lab Sci 1982; 16: 160-94.
24. 24 Phosphatase isoenzymes. In: Wilkinson JH, editor. Isoenzymes. 2nd ed. London: Chapman & Hall, 1970: 239-79.
25. 25 Withold W, Schulte U, Reinauer H. Method for determination of bone alkaline phosphatase activity: analytical performance and clinical usefulness in patients with metabolic and malignant diseases. Clin Chem 1996; 42: 210-7.
26. 26 Price CP, Milligan TP, Darte C. Direct comparison of performance characteristics of two immunoassays for bone isoform of alkaline phosphatase in serum. Clin Chem 1997; 43: 2052-7.
27. 27 Otto VI, Schär BK, Sulser T, Hänseler E. Specific determination of germ cell alkaline phosphatase for early diagnosis and monitoring of seminoma: performance and limitations of different analytical techniques. Clin Chim Acta 1998; 273: 131-47.
28. 28 Hodson AW, Latner AL, Raine L. Iso-enzymes of alkaline phosphatase. Clin Chim Acta 1962; 7: 255-61.
29. 29 Haije WG, De Jong M. Iso-enzyme patterns of serum alkaline phosphatase in agar-gel electrophoresis and their clinical significance. Clin Chim Acta 1963; 8: 620-3.
30. 30 Kaplan MM, Rogers L. Separation of human serum-alkaline-phosphatase isoenzymes by polyacrylamide gel electrophoresis. Lancet 1969; 2: 1029-31.
31. 31 Rhone DP, Mizuno FM. Separation of isoenzymes of alkaline phosphatase by substrate-gel imprint after electrophoresis on cellulose acetate. Clin Chem 1972; 18: 662-6.
32. 32 Fritsche HA, Adams-Park HR. High molecular weight isoenzymes of alkaline phosphatase in human serum. Demonstration by cellulose acetate electrophoresis and physico-chemical characterization. Clin Chim Acta 1974; 52: 81-9.
33. 33 Kalimanovska V, Whitaker KB, Moss DW. Effect of bromelain on alkaline phosphatases of intestinal and non-intestinal tissues and serum. Clin Chim Acta 1987; 170: 219-26.
34. 34 Van Hoof VO, Lepoutre LG, Hoylaerts MF, Chevigné R, De Broe ME. Improved agarose electrophoretic method for separating alkaline phosphatase isoenzymes in serum. Clin Chem 1988; 34: 1857-62.
35. 35 Hamilton BA, McPhee JL, Hawrylak K, Stinson RA. Alkaline phosphatase releasing activity in human tissues. Clin Chim Acta 1989; 186: 249-54.
36. 36 Bentouati L, Baboli MS, Hachem H, Hamza M, Canal P, Soula G. Hyperphosphatasemia related to three intestinal alkaline phosphatase isoforms: biochemical study. Clin Chim Acta 1990; 193: 93-102.
37. 37 Van Hoof VO, Hoylaerts MF, Geryl H, Van Mullem M, Lepoutre LG, De Broe ME. Age and sex distribution of alkaline phosphatase isoenzymes by agarose electrophoresis. Clin Chem 1990; 36: 875-8.
38. 38 Murthy VV, Malikin G, Karmen A. Agarose gel electrophoresis of alkaline phosphatase isoenzymes with wheat germ lectin and fluorogenic substrate. Clin Chem 1991; 37: 1309-10.
39. 39 Rosalki SB, Ying Foo A. Two new methods for separating and quantifying bone and liver alkaline phosphatase isoenzymes in plasma. Clin Chem 1984; 30: 1182-6.
40. 40 Moss DW, Edwards RK. Improved electrophoretic resolution of bone and liver alkaline phosphatases resulting from partial digestion with neuraminidase. Clin Chim Acta 1984; 143: 177-82.
41. 41 Rosalki SB, Ying Foo A. Incubation with neuraminidase and affinity electrophoresis with wheat-germ lectin compared for separating and quantifying alkaline phosphatase isoenzymes in plasma. Clin Chem 1985; 31: 1198-200,
42. 42 Amacher DE, Higgins CV, Schomaker SJ, Clay RJ. Further charaterization of serum alkaline phosphatase from male and female beagle dogs. Enzyme 1989; 42: 1-7.
43. 43 Wei JS, Shih CL, Chung NC, Wang J-Y. Quantitative determination of high molecular weight alkaline phosphatase in patients with colorectal cancer by polyacrylamide gel electrophoresis. Enzyme 1990; 43: 188-91.
44. 44 Griffiths WC, Camara PD, Rosner M, Lev R, Brooks EM. Prevalence and properties of the intestinal alkaline phosphatase identified in serum by cellulose acetate electrophoresis. Clin Chem 1992; 38: 507-11.
45. 45 Romel WC, LaMancusa SJ, Dufrene JK. Detection of serum alkaline phosphatase isoenzymes with phenolphthalein monophosphate following cellulose acetate electrophoresis. Clin Chem 1968; 14: 47-57.
46. 46 Johnson Jr RB. A new fluorometric method for the estimation or detection of total and fractionated alkaline phosphatase. Clin Chem 1969; 15: 108-23.
47. 47 Demetriou JA, Beattie JM. Electrophoretic separation on agarose thin film of isoenzymes of alkaline phosphatase from human serum and tissue. Clin Chem 1971; 17: 290-5.
48. 48 Healy PJ. Quantitative analysis of serum alkaline phosphatase isoenzyme activity. Clin Chim Acta 1974; 55: 407-9.
49. 49 Martins MJ, Dias PO, Hipólito-Reis C, Rat serum alkaline phosphatase electrophoretic fractions: variations with feeding, starvation and cellulose fibre ingestion. Clin Nutr 1998; 17: 279-85.
50. 50 Steele BW, Nord M, Statland BE. Comparative effects of DEA versus AMP buffer on alkaline phosphatase isoenzymes. Clin Chem 1976; 22: 1221.
51. 51 Van Hoof VO, Van Campenhout CM, De Broe ME, Lepoutre LG. Variations in measured alkaline phosphatase activity: influence of isoenzymes and buffer systems. Clin Chem 1990; 36: 2012-3.
52. 52 Lamb EJ, Browne M, John WG, Price CP. Alkaline phosphatase activity measurement in the UK by AMP-buffered methods: an appraisal of current practice. Ann Clin Biochem 1998; 35: 120-7.
53. 53 Fernley HN. Mammalian alkaline phosphatases. In: Boyer PD, editor. The enzymes. Vol IV. New York: Academic Press, 1971: 417-47.
54. 54 Committee on Enzymes of the Scandinavian Society for Clinical Chemistry and Clinical Physiology. Recommended methods for the determination of four enzymes in blood. Scand J Clin Lab Invest 1974; 33: 291-306.
55. 55 International Federation of Clinical Chemistry. Scientific Committee, Analytical Section, Expert Panel on Enzymes. IFCC methods for the measurement of catalytic concentration of enzymes. Part 5. IFCC method for alkaline phosphatase (orthophosphoric-monoester phosphohydrolase, alkaline optimum, EC 3.1.3.1). Clin Chim Acta 1983; 135: 339F-67F. J Clin Chem Clin Biochem 1983; 21; 731-48.
56. 55 International Federation of Clinical Chemistry.Scientific Committee, Analytical Section, ExpertPanel on Enzymes. IFCC methods for themeasurement of catalytic concentration ofenzymes. Part 5. IFCC method for alkalinephosphatase (orthophosphoric-monoester phosphohydrolase, alkaline optimum, EC 3.1.3.1).Clin Chim Acta 1983; 135: 339F-67F. J Clin Chem Clin Biochem 1983; 21; 731-48.
57. 56 Van Hoof VO, Van Mullem M. Comparison of two commercially available systems for the electrophoretic separation of alkaline phosphatase isoenzymes. J Chromatogr 1993; 646: 235-43.