Detection of Transgenes in Crop Plants Using Molecular Beacon Assays

Plant Molecular Biology Reporter

Volumn 17, Issue 4, 1999, Pages 363-370

  Kota, R ^a   Holton, T A ^a   Henry, R J ^a  

^a SOUTHERN CROSS UNIVERSITY   (Australia)

Author keywords

Barley; Molecular beacons; PCR; SNP; Transgenic

Indexed keywords

HORDEUM VULGARE SUBSP. VULGARE;

EID: 0033268004     PISSN: 07359640     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1007602301563     Document Type: Article

References (9)

1. Abedinia M, Henry RJ, Blakeney AB and Lewin L (1997) An efficient transformation system for the Australian rice cultivar, Jarrah. Aust J Plant Physiol 24: 133-141.
2. Bonnet G, Tyagi S, Libchaber A and Kramer FR (1999) Thermodynamic basis of the enhanced specificity of structured DNA probes. Proc Natl Acad Sci USA 96: 6171-6176.
3. Giesendorf BAJ, Vet JAM, Tyagi S, Mensink EJMG, Trijbels FJM and Blom HJ (1998) Molecular beacons: a new approach for semiautomated mutation analysis. Clinical Chemistry 44: 482-486.
4. Higuchi R, Dollinger G, Walsh PS and Griffith R (1992) Simultaneous amplification and detection of specific DNA sequences. Bio-Technology 10: 413-417.
5. Higuchi R, Fockler C, Dollinger G and Watson R (1993) Kinetic PCR analysis: real-time monitoring of DNA amplification reactions. Bio-Technology 11: 1026-1030.
6. Piatek AS, Tyagi S, Pol AC, Telenti A, Miller LP, Kramer FR and Alland D (1998) Molecular beacon sequence analysis for detecting drug resistance in Mycobacteriwn tuberculosis. Nat Biotechnol 16: 359-363.
7. Tingay S, McElroy D, Kalla R, Fieg S, Wang M, Thornton S and Brettell (1997) Agrobacterium tumefaciens-mediated barley transformation. Plant J 11: 1369-1376.
8. Tyagi S and Kramer FR (1996) Molecular beacons: probes that fluoresce upon hybridisation. Nat Biotechnol 14: 303-308.
9. Tyagi S, Bratu D and Kramer FR (1998) Multicolour molecular beacons for allele discrimination. Nat Biotechnol 16: 49-53.