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Volumn 10, Issue 6, 1999, Pages 577-585

DIF signalling and cell fate

Author keywords

Dictyostelium, DIF, DIF dechlorinase, DIF 1 biosynthesis, proportioning model

Indexed keywords

DICTYOSTELIUM;

EID: 0033257044     PISSN: 10849521     EISSN: None     Source Type: Journal    
DOI: 10.1006/scdb.1999.0341     Document Type: Article
Times cited : (67)

References (61)
  • 1
    • 0001294643 scopus 로고
    • Pseudoplasmodium formation and organization in Dictyostelium discoideum
    • Raper KB. Pseudoplasmodium formation and organization in Dictyostelium discoideum. J Elisha Mitchell Sci Soc. 56:1940;241-282.
    • (1940) J Elisha Mitchell Sci Soc , vol.56 , pp. 241-282
    • Raper, K.B.1
  • 2
    • 0015852353 scopus 로고
    • Cell type conversion in isolated prestalk and prespore fragments of the cellular slime mold Dictyostelium discoideum
    • Sakai Y. Cell type conversion in isolated prestalk and prespore fragments of the cellular slime mold Dictyostelium discoideum. Dev Growth Differ. 15:1973;11-19.
    • (1973) Dev Growth Differ , vol.15 , pp. 11-19
    • Sakai, Y.1
  • 3
    • 0017160099 scopus 로고
    • Cell differentiation without morphogenesis in Dictyostelium discoideum
    • Town CD, Gross JD, Kay RR. Cell differentiation without morphogenesis in Dictyostelium discoideum. Nature. 262:1976;717-719.
    • (1976) Nature , vol.262 , pp. 717-719
    • Town, C.D.1    Gross, J.D.2    Kay, R.R.3
  • 4
    • 0345200474 scopus 로고
    • An oligosaccharide-containing factor that induces cell differentiation in Dictyostelium discoideum
    • Town C, Stanford E. An oligosaccharide-containing factor that induces cell differentiation in Dictyostelium discoideum. Proc Natl Acad Sci USA. 76:1979;308-312.
    • (1979) Proc Natl Acad Sci USA , vol.76 , pp. 308-312
    • Town, C.1    Stanford, E.2
  • 5
    • 0021106969 scopus 로고
    • Purification of stalk-cell-inducing morphogens from Dictyostelium discoideum
    • Kay RR, Dhokia B, Jermyn KA. Purification of stalk-cell-inducing morphogens from Dictyostelium discoideum. Eur J Biochem. 136:1983;51-56.
    • (1983) Eur J Biochem , vol.136 , pp. 51-56
    • Kay, R.R.1    Dhokia, B.2    Jermyn, K.A.3
  • 6
    • 0023240287 scopus 로고
    • Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideum
    • Morris HR, Taylor GW, Masento MS, Jermyn KA, Kay RR. Chemical structure of the morphogen differentiation inducing factor from Dictyostelium discoideum. Nature. 328:1987;811-814.
    • (1987) Nature , vol.328 , pp. 811-814
    • Morris, H.R.1    Taylor, G.W.2    Masento, M.S.3    Jermyn, K.A.4    Kay, R.R.5
  • 7
    • 0023955045 scopus 로고
    • Structure elucidation of two differentiation inducing factors (DIF-2 and DIF-3) from the cellular slime mouldDictyostelium discoideum
    • Morris HR, Masento MS, Taylor GW, Jermyn KA, Kay RR. Structure elucidation of two differentiation inducing factors (DIF-2 and DIF-3) from the cellular slime mouldDictyostelium discoideum. Biochem J. 249:1988;903-906.
    • (1988) Biochem J , vol.249 , pp. 903-906
    • Morris, H.R.1    Masento, M.S.2    Taylor, G.W.3    Jermyn, K.A.4    Kay, R.R.5
  • 8
    • 0023868513 scopus 로고
    • Cyclic AMP is an inhibitor of stalk cell differentiation in Dictyostelium discoideum
    • Berks M, Kay RR. Cyclic AMP is an inhibitor of stalk cell differentiation in Dictyostelium discoideum. Dev Biol. 126:1988;108-114.
    • (1988) Dev Biol , vol.126 , pp. 108-114
    • Berks, M.1    Kay, R.R.2
  • 9
    • 0027164470 scopus 로고
    • In vitro stalk cell differentiation in wild-type and 'slugger' mutants of Dictyostelium discoideum
    • Inouye K, Gross J. In vitro stalk cell differentiation in wild-type and 'slugger' mutants of Dictyostelium discoideum. Development. 118:1993;523-526.
    • (1993) Development , vol.118 , pp. 523-526
    • Inouye, K.1    Gross, J.2
  • 10
    • 0023176869 scopus 로고
    • Nature and distribution of the mo rphogen DIF in the Dictyostelium slug
    • Brookman JJ, Jermyn KA, Kay RR. Nature and distribution of the mo rphogen DIF in the Dictyostelium slug. Development. 100:1987;119-124.
    • (1987) Development , vol.100 , pp. 119-124
    • Brookman, J.J.1    Jermyn, K.A.2    Kay, R.R.3
  • 11
    • 0020027102 scopus 로고
    • Developmental regulation of stalk cell differentiation-inducing factor in Dictyostelium discoideum
    • Brookman JJ, Town CD, Jermyn KA, Kay RR. Developmental regulation of stalk cell differentiation-inducing factor in Dictyostelium discoideum. Dev Biol. 91:1982;191-196.
    • (1982) Dev Biol , vol.91 , pp. 191-196
    • Brookman, J.J.1    Town, C.D.2    Jermyn, K.A.3    Kay, R.R.4
  • 12
    • 0021054859 scopus 로고
    • The induction of stalk cell differentiation in submerged monolayers of Dictyostelium discoideum. Characterization of the temporal sequence for the molecular requirement
    • Sobolewski A, Neave N, Weeks G. The induction of stalk cell differentiation in submerged monolayers of Dictyostelium discoideum. Characterization of the temporal sequence for the molecular requirement. Differentiation. 25:1983;93-100.
    • (1983) Differentiation , vol.25 , pp. 93-100
    • Sobolewski, A.1    Neave, N.2    Weeks, G.3
  • 13
    • 0032579553 scopus 로고    scopus 로고
    • The biosynthesis of differentiation-inducing factor, a chlorinated signal molecule regulating Dictyostelium development
    • Kay RR. The biosynthesis of differentiation-inducing factor, a chlorinated signal molecule regulating Dictyostelium development. J Biol Chem. 273:1998;2669-2675.
    • (1998) J Biol Chem , vol.273 , pp. 2669-2675
    • Kay, R.R.1
  • 15
    • 0020540914 scopus 로고
    • A possible morphogen controlling differentiation in Dictyostelium
    • Kay RR, Jermyn KA. A possible morphogen controlling differentiation in Dictyostelium. Nature. 303:1983;242-244.
    • (1983) Nature , vol.303 , pp. 242-244
    • Kay, R.R.1    Jermyn, K.A.2
  • 16
    • 0024553198 scopus 로고
    • Ammonia depletion and DIF trigger stalk cell differentiation in intact Dictyostelium discoideum slugs
    • Wang M, Schaap P. Ammonia depletion and DIF trigger stalk cell differentiation in intact Dictyostelium discoideum slugs. Development. 105:1989;569-574.
    • (1989) Development , vol.105 , pp. 569-574
    • Wang, M.1    Schaap, P.2
  • 17
    • 0024851990 scopus 로고
    • Morphogen hunting in Dictyostelium
    • Kay RR, Berks M, Traynor D. Morphogen hunting in Dictyostelium. Development. 107(Suppl): 1989;81-90.
    • (1989) Development , vol.107 , pp. 81-90
    • Kay, R.R.1    Berks, M.2    Traynor, D.3
  • 19
    • 0021975785 scopus 로고
    • Selective induction of stalk-cell specific proteins in Dictyostelium
    • Kopachik WJ, Dhokia B, Kay RR. Selective induction of stalk-cell specific proteins in Dictyostelium. Differentiation. 28:1985;209-216.
    • (1985) Differentiation , vol.28 , pp. 209-216
    • Kopachik, W.J.1    Dhokia, B.2    Kay, R.R.3
  • 21
    • 0023805043 scopus 로고
    • A Dictyostelium prespore-specific gene is transcriptionally repressed by DIF in vitro
    • Early AE, Williams JG. A Dictyostelium prespore-specific gene is transcriptionally repressed by DIF in vitro. Development. 103:1988;519-524.
    • (1988) Development , vol.103 , pp. 519-524
    • Early, A.E.1    Williams, J.G.2
  • 22
    • 0026023503 scopus 로고
    • Coordinate regulation of the spore coat genes in Dictyostelium discoideum
    • Fosnaugh KL, Loomis WF. Coordinate regulation of the spore coat genes in Dictyostelium discoideum. Dev Genet. 12:1991;123-132.
    • (1991) Dev Genet , vol.12 , pp. 123-132
    • Fosnaugh, K.L.1    Loomis, W.F.2
  • 23
    • 0026772153 scopus 로고
    • DIF-1 induces its own breakdown in Dictyostelium
    • Insall R, Nayler O, Kay RR. DIF-1 induces its own breakdown in Dictyostelium. EMBO J. 11:1992;2849-2854.
    • (1992) EMBO J , vol.11 , pp. 2849-2854
    • Insall, R.1    Nayler, O.2    Kay, R.R.3
  • 24
    • 0025963037 scopus 로고
    • Cyclic nucleotide phosphodiesterase of Dictyostelium discoideum and its glycoprotein inhibitor - Structure and expression of their genes
    • Franke J, Faure M, Wu L, Hall AL, Podgorski GJ, Kessin RH. Cyclic nucleotide phosphodiesterase of Dictyostelium discoideum and its glycoprotein inhibitor - structure and expression of their genes. Dev Genet. 12:1991;104-112.
    • (1991) Dev Genet , vol.12 , pp. 104-112
    • Franke, J.1    Faure, M.2    Wu, L.3    Hall, A.L.4    Podgorski, G.J.5    Kessin, R.H.6
  • 25
    • 0028217453 scopus 로고
    • Dual regulation of the glycogen phosphorylase 2 gene of Dictyostelium discoideum: The effects of DIF-1, cAMP, NH3 and adenosine
    • Yin YH, Rogers PV, Rutherford CL. Dual regulation of the glycogen phosphorylase 2 gene of Dictyostelium discoideum: the effects of DIF-1, cAMP, NH3 and adenosine. Development. 120:1994;1169-1178.
    • (1994) Development , vol.120 , pp. 1169-1178
    • Yin, Y.H.1    Rogers, P.V.2    Rutherford, C.L.3
  • 26
    • 0029879005 scopus 로고    scopus 로고
    • The cAMP receptor subtype cAR2 is restricted to a subset of prestalk cells during Dictyostelium development and displays unexpected DIF-1 responsiveness
    • Saxe III CL, Yu YM, Jones C, Bauman A, Haynes C. The cAMP receptor subtype cAR2 is restricted to a subset of prestalk cells during Dictyostelium development and displays unexpected DIF-1 responsiveness. Dev Biol. 174:1996;202-213.
    • (1996) Dev Biol , vol.174 , pp. 202-213
    • Saxe C.L. III1    Yu, Y.M.2    Jones, C.3    Bauman, A.4    Haynes, C.5
  • 27
    • 0029998855 scopus 로고    scopus 로고
    • Initial cell type divergence in Dictyostelium is independent of DIF-1
    • Shaulsky G, Loomis WF. Initial cell type divergence in Dictyostelium is independent of DIF-1. Dev Biol. 174:1996;214-220.
    • (1996) Dev Biol , vol.174 , pp. 214-220
    • Shaulsky, G.1    Loomis, W.F.2
  • 28
    • 0023791673 scopus 로고
    • Differentiation-inducing factor from the slime mould Dictyostelium discoideum and its analogues
    • Masento MS, Morris HR, Taylor GW, Johnson SJ, Skapski AC, Kay RR. Differentiation-inducing factor from the slime mould Dictyostelium discoideum and its analogues. Biochem J. 256:1988;23-28.
    • (1988) Biochem J , vol.256 , pp. 23-28
    • Masento, M.S.1    Morris, H.R.2    Taylor, G.W.3    Johnson, S.J.4    Skapski, A.C.5    Kay, R.R.6
  • 29
    • 0020536204 scopus 로고
    • Intracellular pH and the control of cell differentiation in Dictyostelium discoideum
    • Gross JD, Bradbury J, Kay RR, Peacey MJ. Intracellular pH and the control of cell differentiation in Dictyostelium discoideum. Nature. 303:1983;244-245.
    • (1983) Nature , vol.303 , pp. 244-245
    • Gross, J.D.1    Bradbury, J.2    Kay, R.R.3    Peacey, M.J.4
  • 30
    • 0022753583 scopus 로고
    • 31P nuclear magnetic resonance study of its regulation and possible role in controlling cell differentiation
    • 31P nuclear magnetic resonance study of its regulation and possible role in controlling cell differentiation. J Cell Sci. 83:1986;165-179.
    • (1986) J Cell Sci , vol.83 , pp. 165-179
    • Kay, R.R.1    Gadian, D.G.2    Williams, S.R.3
  • 31
    • 0000569973 scopus 로고
    • Differences in cytoplasmic pH and the sensitivity to acid load between prespore cells and prestalk cells ofDictyostelium
    • Inouye K. Differences in cytoplasmic pH and the sensitivity to acid load between prespore cells and prestalk cells ofDictyostelium. J Cell Sci. 91:1988;109-115.
    • (1988) J Cell Sci , vol.91 , pp. 109-115
    • Inouye, K.1
  • 32
    • 0029017037 scopus 로고
    • Mitochondrial DNA replication but no nuclear DNA replication during development of Dictyostelium
    • Shaulsky G, Loomis WF. Mitochondrial DNA replication but no nuclear DNA replication during development of Dictyostelium. Proc Natl Acad Sci USA. 92:1995;5660-5663.
    • (1995) Proc Natl Acad Sci USA , vol.92 , pp. 5660-5663
    • Shaulsky, G.1    Loomis, W.F.2
  • 33
    • 0015768073 scopus 로고
    • The calcium content of the cellular slime mold, Dictyostelium discoideum, during development and differentiation
    • Maeda Y, Maeda M. The calcium content of the cellular slime mold, Dictyostelium discoideum, during development and differentiation. Exp Cell Res. 82:1973;125-130.
    • (1973) Exp Cell Res , vol.82 , pp. 125-130
    • Maeda, Y.1    Maeda, M.2
  • 34
    • 0029077310 scopus 로고
    • Patterns of free calcium in multicellular stages of Dictyostelium expressing jellyfish apoaequorin
    • Cubitt AB, Firtel RA, Fischer G, Jaffe LF, Miller AL. Patterns of free calcium in multicellular stages of Dictyostelium expressing jellyfish apoaequorin. Development. 121:1995;2291-2301.
    • (1995) Development , vol.121 , pp. 2291-2301
    • Cubitt, A.B.1    Firtel, R.A.2    Fischer, G.3    Jaffe, L.F.4    Miller, A.L.5
  • 36
    • 0029791315 scopus 로고    scopus 로고
    • 2+levels mediates stalk gene induction by differentiation inducing factor in Dictyostelium
    • 2+levels mediates stalk gene induction by differentiation inducing factor in Dictyostelium. EMBO J. 15:1996;5177-5183.
    • (1996) EMBO J , vol.15 , pp. 5177-5183
    • Schaap, P.1    Nebl, T.2    Fisher, P.R.3
  • 37
    • 0020134133 scopus 로고
    • CAMP and spore differentiation in Dictyostelium discoideum
    • Kay RR. cAMP and spore differentiation in Dictyostelium discoideum. Proc Natl Acad Sci USA. 79:1982;3228-3231.
    • (1982) Proc Natl Acad Sci USA , vol.79 , pp. 3228-3231
    • Kay, R.R.1
  • 38
    • 0028170061 scopus 로고
    • +concentrations determine cell fate in Dictyostelium discoideum
    • +concentrations determine cell fate in Dictyostelium discoideum. FASEB J. 8:1994;869-874.
    • (1994) FASEB J , vol.8 , pp. 869-874
    • Kubohara, Y.1    Okamoto, K.2
  • 39
    • 0031771555 scopus 로고    scopus 로고
    • Two distinct signaling pathways mediate DIF induction of prestalk gene expression in Dictyostelium
    • Verkerke-van Wijk I, Brandt R, Bosman L, Schaap P. Two distinct signaling pathways mediate DIF induction of prestalk gene expression in Dictyostelium. Exp Cell Res. 245:1998;179-185.
    • (1998) Exp Cell Res , vol.245 , pp. 179-185
    • Verkerke-Van Wijk, I.1    Brandt, R.2    Bosman, L.3    Schaap, P.4
  • 40
    • 0025354308 scopus 로고
    • New roles for DIF? Effects on early development in Dictyostelium
    • Wurster B, Kay RR. New roles for DIF? Effects on early development in Dictyostelium. Dev Biol. 140:1990;189-195.
    • (1990) Dev Biol , vol.140 , pp. 189-195
    • Wurster, B.1    Kay, R.R.2
  • 41
    • 0025076451 scopus 로고
    • A specific DIF binding protein in Dictyostelium
    • Insall R, Kay RR. A specific DIF binding protein in Dictyostelium. EMBO J. 9:1990;3323-3328.
    • (1990) EMBO J , vol.9 , pp. 3323-3328
    • Insall, R.1    Kay, R.R.2
  • 43
    • 0032528263 scopus 로고    scopus 로고
    • Developmentally and spatially regulated activation of a Dictyostelium STAT protein by a serpentine receptor
    • Araki T, Gamper M, Early A, Fukuzawa M, Abe T, Kawata T, Kim E, Firtel RA, Williams JG. Developmentally and spatially regulated activation of a Dictyostelium STAT protein by a serpentine receptor. EMBO J. 17: 1998;4018-4028.
    • (1998) EMBO J , vol.17 , pp. 4018-4028
    • Araki, T.1    Gamper, M.2    Early, A.3    Fukuzawa, M.4    Abe, T.5    Kawata, T.6    Kim, E.7    Firtel, R.A.8    Williams, J.G.9
  • 44
    • 0032585340 scopus 로고    scopus 로고
    • The Dictyostelium developmental cDNA project: Generation and analysis of expressed sequence tags from the first-finger stage of development
    • Morio T et al. The Dictyostelium developmental cDNA project: generation and analysis of expressed sequence tags from the first-finger stage of development. DNA Res. 5:1998;335-340.
    • (1998) DNA Res , vol.5 , pp. 335-340
    • Morio, T.1
  • 45
    • 0025759050 scopus 로고
    • The DIF-1 signaling system in Dictyostelium -metabolism of the signal
    • Traynor D, Kay RR. The DIF-1 signaling system in Dictyostelium -metabolism of the signal. J Biol Chem. 266:1991;5291-5297.
    • (1991) J Biol Chem , vol.266 , pp. 5291-5297
    • Traynor, D.1    Kay, R.R.2
  • 46
    • 0028925391 scopus 로고
    • The proximal pathway of metabolism of the chlorinated signal molecule differentiation-inducing factor-1 (DIF-1) in the cellular slime mould Dictyostelium
    • Morandini P, Offer J, Traynor D, Nayler O, Neuhaus D, Taylor GW, Kay RR. The proximal pathway of metabolism of the chlorinated signal molecule differentiation-inducing factor-1 (DIF-1) in the cellular slime mould Dictyostelium. Biochem J. 306:1995;735-743.
    • (1995) Biochem J , vol.306 , pp. 735-743
    • Morandini, P.1    Offer, J.2    Traynor, D.3    Nayler, O.4    Neuhaus, D.5    Taylor, G.W.6    Kay, R.R.7
  • 48
    • 0026733571 scopus 로고
    • Differentiation-inducing-factor dechlorinase, a novel cytosolic dechlorinating enzyme from Dictyostelium discoideum
    • Nayler O, Insall R, Kay RR. Differentiation-inducing-factor dechlorinase, a novel cytosolic dechlorinating enzyme from Dictyostelium discoideum. Eur J Biochem. 208:1992;531-536.
    • (1992) Eur J Biochem , vol.208 , pp. 531-536
    • Nayler, O.1    Insall, R.2    Kay, R.R.3
  • 49
    • 0027458297 scopus 로고
    • A localized differentiation-inducing-factor sink in the front of the Dictyostelium slug
    • Kay RR, Large S, Traynor D, Nayler O. A localized differentiation-inducing-factor sink in the front of the Dictyostelium slug. Proc Natl Acad Sci USA. 90:1993;487-491.
    • (1993) Proc Natl Acad Sci USA , vol.90 , pp. 487-491
    • Kay, R.R.1    Large, S.2    Traynor, D.3    Nayler, O.4
  • 50
    • 0028856336 scopus 로고
    • Evidence for positional differentiation of prestalk cells and for a morphogenetic gradient inDictyostelium
    • Early A, Abe T, Williams J. Evidence for positional differentiation of prestalk cells and for a morphogenetic gradient inDictyostelium. Cell. 83:1995;91-99.
    • (1995) Cell , vol.83 , pp. 91-99
    • Early, A.1    Abe, T.2    Williams, J.3
  • 52
    • 0015833188 scopus 로고
    • Cell sorting out during the differentiation of mixtures of metabolically distinct populations of Dictyostelium discoideum
    • Leach CK, Ashworth JM, Garrod DR. Cell sorting out during the differentiation of mixtures of metabolically distinct populations of Dictyostelium discoideum. J Embryol Exp Morphol. 29:1973;647-661.
    • (1973) J Embryol Exp Morphol , vol.29 , pp. 647-661
    • Leach, C.K.1    Ashworth, J.M.2    Garrod, D.R.3
  • 53
    • 0021646591 scopus 로고
    • Dependence of cell-type proportioning and sorting on cell cycle phase in Dictyostelium discoideum
    • Weijer CJ, Duschl G, David CN. Dependence of cell-type proportioning and sorting on cell cycle phase in Dictyostelium discoideum. J Cell Sci. 70:1984;133-145.
    • (1984) J Cell Sci , vol.70 , pp. 133-145
    • Weijer, C.J.1    Duschl, G.2    David, C.N.3
  • 55
    • 0027348412 scopus 로고
    • Lateral inhibition and pattern formation in Dictyostelium
    • Loomis WF. Lateral inhibition and pattern formation in Dictyostelium. Curr Topics Dev Biol. 28:1993;1-46.
    • (1993) Curr Topics Dev Biol , vol.28 , pp. 1-46
    • Loomis, W.F.1
  • 56
    • 0017302290 scopus 로고
    • Tip formation is regulated by an inhibitory gradient in the Dictyostelium discoideum slug
    • Durston AJ. Tip formation is regulated by an inhibitory gradient in the Dictyostelium discoideum slug. Nature. 263:1976;126-129.
    • (1976) Nature , vol.263 , pp. 126-129
    • Durston, A.J.1
  • 57
    • 0025255873 scopus 로고
    • A spatial gradient of expression of a cAMP-regulated prespore cell type-specific-gene in Dictyostelium
    • Haberstroh L, Firtel RA. A spatial gradient of expression of a cAMP-regulated prespore cell type-specific-gene in Dictyostelium. Genes Dev. 4:1990;596-612.
    • (1990) Genes Dev , vol.4 , pp. 596-612
    • Haberstroh, L.1    Firtel, R.A.2
  • 59
    • 0025221128 scopus 로고
    • Combinatorial control of cell differentiation by cAMP and DIF-1 during development of Dictyostelium discoideum
    • Berks M, Kay RR. Combinatorial control of cell differentiation by cAMP and DIF-1 during development of Dictyostelium discoideum. Development. 110:1990;977-984.
    • (1990) Development , vol.110 , pp. 977-984
    • Berks, M.1    Kay, R.R.2
  • 60
    • 0023203497 scopus 로고
    • Two distinct classes of prestalk-enriched mRNA sequences in Dictyostelium discoideum
    • Jermyn KA, Berks M, Kay RR, Williams JG. Two distinct classes of prestalk-enriched mRNA sequences in Dictyostelium discoideum. Development. 100:1987;745-755.
    • (1987) Development , vol.100 , pp. 745-755
    • Jermyn, K.A.1    Berks, M.2    Kay, R.R.3    Williams, J.G.4
  • 61
    • 0027525351 scopus 로고
    • Prestalk cells in monolayer cultures exhibit two distinct modes of cellulose synthesis during stalk cell differentiation in Dictyostelium
    • Blanton RL. Prestalk cells in monolayer cultures exhibit two distinct modes of cellulose synthesis during stalk cell differentiation in Dictyostelium. Development. 119:1993;703-710.
    • (1993) Development , vol.119 , pp. 703-710
    • Blanton, R.L.1


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