NIH 3T3 cells malignantly transformed by mot-2 show inactivation and cytoplasmic sequestration of the p53 protein

Cell Research

Volumn 9, Issue 4, 1999, Pages 261-269

  Wadhwa, Renu ^a   Takano, Syuichi ^b   Mitsui, Youji ^b   Kaul, Sunil C ^b  

^a CHUGAI PHARMACEUTICAL CO LTD   (Japan)

^b NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Malignant transformation; mot 2; NIH 3T3; p53 inactivation

Indexed keywords

MUS MUSCULUS;

HEAT SHOCK PROTEIN 70; HSPA9B PROTEIN, MOUSE; PROTEIN P53;

ANIMAL; ARTICLE; BIOSYNTHESIS; CELL CULTURE; CELL STRAIN 3T3; CELL TRANSFORMATION; CYTOPLASM; DRUG EFFECT; FLUORESCENT ANTIBODY TECHNIQUE; GENE TRANSLOCATION; GENETICS; METABOLISM; MOUSE; RADIATION EXPOSURE; RADIATION RESPONSE; ULTRAVIOLET RADIATION;

3T3 CELLS; ANIMALS; CELL TRANSFORMATION, NEOPLASTIC; CELLS, CULTURED; CYTOPLASM; DOSE-RESPONSE RELATIONSHIP, RADIATION; FLUORESCENT ANTIBODY TECHNIQUE; HSP70 HEAT-SHOCK PROTEINS; MICE; TRANSLOCATION, GENETIC; TUMOR SUPPRESSOR PROTEIN P53; ULTRAVIOLET RAYS;

EID: 0033256455     PISSN: 10010602     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.cr.7290025     Document Type: Article

References (27)

1. Levine AJ. p53, the cellular gatekeeper for growth and division. Cell 1997; 88:323-31.
2. Harvey DM, Levine AJ. p53 alteration is a common event in the spontaneous immortalization of primary BALB/c murine embryo fibroblasts. Genes Dev 1991; 5:2375-85.
3. Metz T, Harris AW, Adams JM. Absence of p53 allows direct immortalization of hematopoietic cells by the myc and raf oncogenes. Cell 1995; 82:29-36.
4. ynford-Thomas D. Telomeres, p53 and cellular senescence, Oncology Res. 1996; 8:387-98.
5. Donehower LA, Bradley A. The tumor suppressor p53. Biochim Biophy Acta 1993; 23:181-205.
6. Adler V, Pincus MR, Minamoto T, Fuchs SY, Bluth MJ, Brandt -Rauf PW, Friedman FK, Robinson RC, Chen JM, Wang XW, Harris CC, Ronai Z. Conformation-dependent phosphorylation of p53. Proc Natl Acad Science USA 1997; 94:1686-91.
7. Wadhwa R, Takano S, Robert M, Yoshida A, Reddel RR, Nomura H, Mitsui Y, Kaul SC. Inactivation of tumor suppressor p53 by mot-2, an hsp70 family member. J Biol Chem 1998; 273:29586-91.
8. Hinds PW, Finlay CA, Frey AB, Levine AJ. Immunological evidence for the association of p53 with a heat shock protein, hsc70, in p53-plus-ras-transformed cell lines. Mol Cell Biol 1987; 7:2863-9.
9. Kaur J, Srivastava A, Ralhan R. p53-HSP70 complexes in oral dysplasia and cancer: potential prognostic implications. Europ J Cancer B Oral Oncol 1996; 32:45-9.
10. Sugito K, Yamane M, Hattori H, Hayashi Y, Tohnai I, Ueda M, Tsuchida N. and Ohtsuka K. Interaction between hsp70 and hsp40, eukaryotic homologues of DnaK and DnaJ, in human cells expressing mutant-type p53. FEBS Lett 1995; 358:161-4.
11. Hainaut P, Milner J. Interaction of heat-shock protein 70 with p53 translated in vitro: evidence for interaction with dimeric p53 and for a role in the regulation of p53 conformation. EMBO J 1992; 11:3513-20.
12. Fourie AM, Hupp TR, Lane DP, Sang BC, Barbosa MS, Sambrook JF, Gething MJ. HSP70 binding sites in the tumor suppressor protein p53. J Biol Chem 1997; 272:19471-9.
13. Merrick BA, He C, Witcher LL, Patterson RM, Reid JJ, Pence -Pawlowski PM, Selkirk JK. HSP binding and mitochondrial localization of p53 protein in human HT1080 and mouse C3H10T1/2 cell lines. Biochim Biophys Acta 1996; 13:57-68.
14. Wadhwa R, Kaul SC, Ikawa Y, Sugimoto Y. Identification of a novel member of mouse hsp70 family. Its association with cellular mortal phenotype. J Biol Chem 1993; 268:6615-21.
15. Wadhwa R, Kaul SC, Mitsui Y, Sugimoto Y. Differential subcellular distribution of mortalin in mortal and immortal mouse and human fibroblasts. Exp Cell Res 1993; 207:442-8.
16. Wadhwa R, Kaul SC, Sugimoto Y, Mitsui Y. Induction of cellular senescence by transfection of cytosolic mortalin cDNA in NIH 3T3 cells. J Biol Chem 1993; 268:22239-42.
17. Kaul SC, Matsui M, Takano S, Sugihara T, Mitsui Y, Wadhwa R. Expression analysis of mortalin, a unique member of the Hsp70 family of proteins, in rat tissues. Exp Cell Res 1997; 232:56-63.
18. Takano S, Wadhwa R, Yoshii Y, Nose T, Kaul SC, Mitsui Y. Elevated levels of mortalin expression in human brain tumors. Exp Cell Res 1997; 237:38-45.
19. Kaul SC, Mitsui Y, Wadhwa R. Molecular insights to cellular mortality and immortalization. Ind J Exp Biol 1998; 36:345-52.
20. Wadhwa R, Kaul SC, Mitsui Y. (1999) Cellular mortality and immortalization: a complex interplay of multiple gene functions. In: Progress in Molecular and Subcellular Biology, Vol. 24, Cell Immortalization (Edited by Dr. A. Macieira-Coelho) Springer Verlag, Germany. 191-204.
21. Kaul SC, Duncan EL, Englezou A, Takano S, Reddel RR, Mitsui Y, Wadhwa R. Malignant transformation of NIH3T3 cells by overexpression of mot-2 protein. Oncogene 1998; 17:907-11.
22. El-Deiry WS, Tokino T, Velculescu VE, Levy DB, Parsons R, Trent JM, Lin D, Mercer WE, Kinzler KW, Vogelstein, B. WAF1, a potential mediator of p53 tumor suppression. Cell 1993; 75:817-25.
23. Fay FS, Carrington W, Iogarty KE. Three-dimensional molecular distribution in single cells analyzed using the digital imaging microscope. J Microsc 1989; 153:133-49.
24. Kaul SC, Wadhwa R, Sugihara T, Obuchi K, Komatsu Y, Mitsui Y. Identification of genetic events involved in early steps of immortalization of mouse fibroblasts. Biochim Biophys Acta 1994; 1201:389-96.
25. Kashanchi F, Araujo J, Doniger J, Muralidhar S, Hoch R, Khleif S, Mendelson E, Thompson J, Azumi N, Brady JN, Luppi M, Torelli G, Rosenthal J. Human herpesvirus 6 [HHV-6] ORF-1 transactivating gene exhibits malignant transforming activity and its protein binds to p53. Oncogene 1997; 14:359-67.
26. Moll UM, Ostermeyer AG, Haladay R, Winkfield B, Frazier M, Zambetti, G. Cytoplasmic sequestration of wild-type p53 protein impairs the G1 checkpoint after DNA damage. Mol Cell Biol 1996; 16:1126-37.
27. Ostermeyer AG, Runko E, Winkfield B, Ahn B, Moll UM. Cytoplasmically sequestered wild-type p53 protein in neuroblastoma is relocated to the nucleus by a C-terminal peptide Proc. Natl Acad Sci USA 1996; 93:15190-94.