Inhibition of expression of the Galα 1-3Gal epitope on porcine cells using an intracellular single-chain antibody directed against α1,3Galactosyltransferase

Journal of Immunological Methods

Volumn 231, Issue 1-2, 1999, Pages 191-205

  Sepp, Armin ^a   Farrar, Conrad A ^a   Dorling, Tony ^a   Cairns, Tom ^a   George, Andrew J T ^a   Lechler, Robert I ^a  

^a IMPERIAL COLLEGE SCHOOL OF MEDICINE   (United Kingdom)

Author keywords

1,3Galactosyltransferase; Glycosylation; Single chain antibody; Xenotransplantation

Indexed keywords

ANTIBODY; EPITOPE; GALACTOSYLTRANSFERASE; COMPLEMENT; DISACCHARIDE; GALACTOSYL (1 3)GALACTOSE; GALACTOSYL-(1-3)GALACTOSE; HYBRID PROTEIN; IMMUNOGLOBULIN FRAGMENT; LYSYL ASPARTYL GLUTAMYL LEUCINE; LYSYL-ASPARTYL-GLUTAMYL-LEUCINE; N ACETYLLACTOSAMINIDE ALPHA 1,3 GALACTOSYLTRANSFERASE; N-ACETYLLACTOSAMINIDE ALPHA-1,3-GALACTOSYLTRANSFERASE; OLIGOPEPTIDE; PEPTIDE LIBRARY; SIGNAL PEPTIDE;

ANTIBODY AFFINITY; ANTIGEN EXPRESSION; ARTICLE; ENDOTHELIUM CELL; GLYCOSYLATION; GRAFT REJECTION; KNOCKOUT GENE; NONHUMAN; PHAGE DISPLAY; PRIORITY JOURNAL; XENOTRANSPLANTATION; AMINO ACID SEQUENCE; ANIMAL; CELL CULTURE; CELL STRAIN COS1; GENE EXPRESSION REGULATION; GENETICS; HUMAN; IMMUNOGLOBULIN VARIABLE REGION; IMMUNOLOGY; INTRACELLULAR FLUID; MOLECULAR CLONING; MOLECULAR GENETICS; SWINE;

AMINO ACID SEQUENCE; ANIMALS; CELLS, CULTURED; CLONING, MOLECULAR; COMPLEMENT SYSTEM PROTEINS; COS CELLS; DISACCHARIDES; EPITOPES, B-LYMPHOCYTE; GALACTOSYLTRANSFERASES; GENE EXPRESSION REGULATION; HUMANS; IMMUNOGLOBULIN FRAGMENTS; IMMUNOGLOBULIN VARIABLE REGION; INTRACELLULAR FLUID; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PEPTIDE LIBRARY; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; SWINE;

EID: 0033233170     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(99)00134-9     Document Type: Article

References (49)

1. Beerli R.R., Wels W., Hynes N.E. Intracellular expression of single chain antibodies reverts ErB-2 transformation, JBC 269, no39, 23931-23936, 1994. J. Biol. Chem. 269:1994;23931.
2. Blakely M.L., Van der Werf W.J., Berndt M.C., Dalmasso A.P., Bach F.H., Hancock W.W. Activation of intragraft endothelial and mononuclear cells during discordant xenograft rejection. Transplantation. 58:1994;1059.
3. Blanken M.M., Van den Eijnden D.H. Biosynthesis of terminal Galα1-3Galβ1-4GlcNAc-R oligosaccharide sequences of glycoconjugates. J. Biol. Chem. 260:1985;12927.
4. Brinkmann U., Pai L.P., FitzGerald D.J., Willingham M., Pastan I. B3(Fv)-PE38KDEL, a single-chain immunotoxin that causescomplete regression of a human carcinoma in mice. Proc. Natl. Acad. Sci. USA. 88:1991;8616.
5. Cairns T., Karlsson E., Holgersson J., Taube D., Welsh K., Samuelsson G. Confirmation of a major target epitope of human natural IgG and IgM anti-pig antibodies: terminal galactose α-1,3-galactose. Transplant. Proc. 26:1994;1384.
6. Cooper D.K., Good A.H., Koren E., Oriol R., Malcolm A.J., Ippolito R.M., Neethling F.A., Ye Y., Romano E., Zuhdi N. Identification of α-galactosyl and other carbohydrate epitopes that are bound by human anti-pig antibodies: relevance to discordant xenografting in man. Transplant. Immunol. 1:1993;198.
7. Dalmasso A.P., Vercellotti G.M., Fischel R.J., Bolman R.M., Bach F.H., Platt J.L. Mechanism of complement activation in the hyperacute rejection of porcine organs transplanted into primate recipients. Am. J. Pathol. 140:1992;1157.
8. Galili U. Interaction of the natural anti-gal antibody with α-galactosyl epitopes - a major obstacle for xenotransplantation in humans. Immunol. Today. 14:1993;480.
9. Galili U., Clark M.R., Shohet S.B., Buehler J. Evolutionary relationship between the natural anti-Gal antibody and the Galα1,3Gal epitope in primates. Proc. Natl. Acad. Sci. USA. 84:1987;1369.
10. Galili U., Shohet S.B., Kobrin E., Stults C.S.M., Macher B.A. Man, apes and Old-world monkeys differ from other mammals in the expressin of α-Galactosyl epitopes on nucleated cells. J. Biol. Chem. 263:1988;17755.
11. George A.J.T., French R.R., Glenie M.J. Measurement of kinetic binding constants of a panel of anti-saporin antibodies using a resonant mirror biosensor. J. Immunol. Methods. 183:1995;51.
12. Haataja S., Tikkanen K., Hytonen J., Finne J. The Galα1-4Gal-binding adhesion of Streptococcus suis, a gram-positive meningitis-associated bacterium. J. Adv. Exp. Med. Biol. 408:1996;25.
13. Hayashi S., Isobe K., Emi N., Yokoyama I., Takagi H. Inhibition of species-specific complement-mediated cytolysis in xenoendothelial cells transfected with GPI-anchored complement regulatory factor (DAF, HRF20) gene using retroviral vector: comparison between single (DAF or HRF20) and double (DAF and HRF20) transfected xenoendothelial cells. Transplant. Proc. 26:1994;1243.
14. Hecklostreicher B., Binder R., Kirschfink M. Functional activity of the membrane-associated complement inhibitor CD59 in a pig-to-human in vitro model for hyperacute xenograft rejection. Clin. Exp. Immunol. 102(3):1995;589.
15. Hendriks S.P., He P., Stults C.L.M., Macher B.A. Regulation of the expression of Galα1,3Galβ1,4GlcNac glycosphingolipids in kidney. J. Biol. Chem. 265:1990;17621.
16. Henion T.R., Macher B.A., Anaraki F., Galili U. Defining the minimal size of catalytically active primate α1,3galactosyltransferase: structure-function studies on the recombinant truncated enzyme. Glycobiology. 4:1994;193.
17. Hoogenboom H.R., Griffiths A.D., Johnson K.S., Chiswell D.J., Hudson P., Winter G. Multi-subunit proteins on the surface of filamentous phage: methologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res. 19:1991;4133.
18. Kennedy S.P., Rollins S.A., Burton W.V., Sims P.J., Bothwell A.L.M., Squinto S.P., Zavoico G.B. Protection of porcine aortic endothelial-cells from complement-mediated cell-lysis and activation by recombinant human CD59. Transplantation. 57:1994;1494.
19. Kirk A.D., Hall B.L., Finn O.J., Bollinger R.R. In vitro analysis of the human antiporcine t-cell repertoire. Transplant. Proc. 24:1992;602.
20. Kirk A.D., Li R.A., Kinch M.S., Abernethy K.A., Doyle C., Bollinger R.R. The human antiporcine cellular repertoire - in vitro studies of acquired and innate cellular responsiveness. Transplantation. 55:1993;924.
21. LaTemple D.C., Henion T.R., Anaraki F., Galili U. Synthesis of α-galactosyl epitopes by recombinant α1,3galactosyl transferase for opsonization of human tumor cell vaccines by anti-galactose. Cancer Res. 56:1996;3069.
22. LaVallie E.R., DiBlasio E.A., Kovacic S., Grant K.L., Schendel P.F., McCoy J.M. A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio/Technology. 11:1996;187.
23. McCurry K.R., Kooyman D.L., Alvarado C.G., Cotterell A.H., Martin M.J., Logan J.S., Platt J.L. Human complement regulatory proteins protect swine-to-primate cardiac xenografts from humoral injury. Nat. Med. 1:1995;423.
24. Miyagawa S., Shirakura R., Iwata K., Nakata S., Matsumiya G., Izutani H., Matsuda H., Terado A., Matsumoto M., Nagasawa S.et al. Effects of transfected complement regulatory proteins, MCP, DAF and MCP/DAE hybrid, on complement-mediated swine endothelial cell lysis. Transplantation. 58:1994;834.
25. Murphy L.A., Goldstein I.J. Five α-[D-Galactopyronosy]-binding isolectins from Bandeiraea simplicifolia seeds. J. Biol. Chem. 252:1977;4739.
26. Nissim A., Hoogenboom H.R., Tomlinson I.M., Flynn G., Midgley C., Lane D., Winter G. Antibody fragments from a 'single pot' phage display library as immunochemical reagents. EMBO J. 13:1994;692.
27. Palmetshofer A., Galili U., Dalmasso A.P., Robson S.C., Bach F.H. α-Galactosyl epitope-mediated activation of porcine aortic endothelial cells: type I activation. Transplantation. 65:1998;844.
28. Palmetshofer A., Galili U., Dalmasso A.P., Robson S.C., Bach F.H. α-Galactosyl epitope-mediated activation of porcine aortic endothelial cells: type II activation. Transplantation. 65:1998;971.
29. Platt J.L., Turman M.A., Noreen H.J., Fischel R.J., Bolman R.M., Bach F.H. An elisa assay for xenoreactive natural antibodies. Transplantation. 49:1990;1000.
30. Platt J.L., Lindman B.J., Geller R.L., Noreen H.J., Swanson J.L., Dalmasso A.P., Bach F.H. The role of natural antibodies in the activation of xenogenic endothelial-cells. Transplantation. 52:1991;1037.
31. Pruitt S.K., Baldwin W.M., Marsh H.C., Lin S.S., Yeh C.G., Bollinger R.R. The effect of soluble complement receptor type-1 on hyperacute xenograft rejection. Transplantation. 52:1991;868.
32. Richardson J.H., Sodroski J.G., Waldman T.A., Marasco W.A. Phenotypic knockout of the high-affinity human interleukin 2 receptor by intracellular single-chain antibodies against the a subunit of the receptor. Proc. Natl. Acad. Sci. USA. 92:1995;3137.
33. Rose A.G., Cooper D.K.C., Human P.A. Histopathology of hyperacute rejection of the heart: experimental and clinical observations in allografts and xenografts. J. Heart Lung Transplant. 10:1991;223.
34. Rosengard A.M., Cary N., Horlsey J., Belcher C., Langford G., Cozzi E., Wallwork J., White D.J.G. Endothelial expression of human decay accelerating factor in transgenic pig tissue: a potential approach for human complement inactivation in discordant xenografts. Transplant. Proc. 27:1995;326.
35. Sambrook, J., Fritch, E.F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbour Laboratory Press.
36. Sandrin M.S., McKenzie I.F. Galα(1,3)Gal, the major xenoantigen(s) recognised in pigs by human natural antibodies. Immunol. Rev. 141:1994;169.
37. Sandrin M.S., Vaughan H.A., Dabkowski P.L., McKenzie I.F.C. Anti-pig IgM antibodies in human serum react predominantly with Gal(α-1-3)Gal epitopes. Proc. Natl. Acad. Sci. USA. 90:1993;11391.
38. Sandrin M.S., Fodor W.L., Mouhtouris E., Osman N., Cohney S., Rollins S.A., Guilmette E.R., Setter E., Squinto S.P., McKenzie I.F.C. Enzymatic remodelling of the carbohydrate surface of a xenogenic cell substantially reduces human antibody binding and complement-mediated cytolysis. Nat. Med. 1(12):1995;1261.
39. Schachter, H., 1994. Molecular cloning of glycosyltransferase genes. In: Fukuda, M., Hindsgaul, O. (Eds.), Molecular Glycobiology. IRL Press, Oxford, p. 88.
40. Sepp A., Binns R.M., Lechler R.I. Improved protocol for colorimetric detection of complement-mediated cytotoxicity based on the measurement of cytoplasmic lactate dehydrogenase activity. J. Immunol. Methods. 196:1996;175.
41. Sepp A., Skacel P., Lindstedt R., Lechler R. Expression of α1,3-Galactose and other type 2 oligosaccharide structures in a porcine endothelial cell line transfected with human α1,2-fucosyltransferase cDNA. J. Biol. Chem. 272:1997;23104.
42. Shinkel T.A., Chen C.G., Salvaris E., Henion T.R., Barlow H., Galili U., Pearse M.J., d'Apice A.J. Changes in cell surface glycosylation in α1,3-galactosyltransferase knockout and α1,2-fucosyltransferase transgenic mice. Transplantation. 64:1997;197.
43. Strahan K.M., Gu F., Preece A.F., Gustavsson I., Andersson L., Gustafsson K. cDNA sequence and chromosome localization of pig alpha 1,3Galactosyltransferase. Immunogenetics. 41:1995;101.
44. Thall A., Etienne-Decerf J., Winand R., Galili U. The α-galactosyl epitope on mammalian thyroid cells. Acta Endocrinol. 124:1991;692.
45. Vanhove B., Charreau B., Cassard A., Pourcel C., Soulillou J.-P. Intracellular expression in pig cells of anti-α1,3Galactosyltransferase single-chain Fv antibodies reduces Galα1,3Gal expression and inhibits cytotoxicity mediated by anti-Gal xenoantibodies. Transplantation. 66:1998;1477.
46. Wong-Madden S.T., Landry D. Purification and characterization of novel glycosidases from the bacterial genus Xanthomonas. Glycobiology. 5:1995;19.
47. 4 for Galα1-4Gal ligand. Biochem. Biophys. Res. Commun. 216:1995;814.
48. Yannoutsos N., Langford G.A., Cozzi E., Lancaster R., Elsome K., Chen P., White D.J.G. Production of pigs transgenic for human regulators of complement activation. Transplant. Proc. 27:1995;324.
49. Yuan Q., Strauch K.L., Lobb R.R., Hemler M.E. Intracellar single-chain antibody inhibits integrin VLA-4 maturation and function. Biochem. J. 318:1996;591.