Formation of mineralized bone nodules by rat calvarial osteoblasts decreases with donor age due to a reduction in signaling through EP1 subtype of prostaglandin E2 receptor

Journal of Cellular Biochemistry

Volumn 75, Issue 2, 1999, Pages 215-225

  Fujieda, Masaki ^a   Kiriu, Michiaki ^a   Mizuochi, Shigeki ^a   Hagiya, Ken Ichi ^a   Kaneki, Hiroyuki ^a   Ide, Hayao ^a  

^a TOHO UNIVERSITY   (Japan)

Author keywords

Aging; CAMP; Mineralized bone nodule; Osteoblasts; Phosphoinositide turnover; Prostaglandin E2 (PGE2)

Indexed keywords

ALKALINE PHOSPHATASE; CYCLIC AMP; CYCLOOXYGENASE 2 INHIBITOR; FORSKOLIN; INOSITOL 1,4,5 TRISPHOSPHATE; N (2 CYCLOHEXYLOXY 4 NITROPHENYL)METHANESULFONAMIDE; PROSTAGLANDIN E RECEPTOR; PROSTAGLANDIN E2; THYMIDINE; TRITIUM;

AGE; ANIMAL CELL; ARTICLE; BONE MINERALIZATION; CELL CULTURE; CELL DIFFERENTIATION; CELL PROLIFERATION; CONTROLLED STUDY; DONOR; NONHUMAN; OSTEOBLAST; PRIORITY JOURNAL; RAT;

AGE FACTORS; AGING; ALKALINE PHOSPHATASE; ALPROSTADIL; ANIMALS; BIOLOGICAL MARKERS; BONE AND BONES; CELL DIFFERENTIATION; CELL DIVISION; CELLS, CULTURED; DINOPROSTONE; DNA; FEMALE; MODELS, BIOLOGICAL; OSTEOBLASTS; RATS; RATS, WISTAR; RECEPTORS, PROSTAGLANDIN E; SIGNAL TRANSDUCTION; SKULL;

EID: 0033231791     PISSN: 07302312     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-4644(19991101)75:2<215::AID-JCB4>3.0.CO;2-S     Document Type: Article

References (18)

1. Bellows CG, Aubin JE, Heersche JNM, Antosz ME. 1986. Mineralized bone nodules formed in vitro from enzymatically released rat calvaria cell populations. Calcif Tissue Int 38:143-154.
2. Bhargava U, Bar-Lev M, Bellows CG, Aubin JE. 1988. Ultrastructural analysis of bone nodules formed in vitro by isolated fetal rat calvaria cells. Bone 9:155-163.
3. Centrella M, Casinghino S, McCarthy T. 1994. Differential actions of prostaglandins in separate cell populations from fetal rat bone. Endocrinology 135:1611-1620.
4. 2. Prostaglandins 27:96-103.
5. Coleman RA, Smith WL, Narumiya S. 1994. International union of pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes. Pharmacol Rev 46:205-229.
6. 2 stimulates DNA synthesis by a cyclic AMP-independent pathway in osteoblastic clone MC3T3-E1 cells. J Cell Physiol 128:155-161.
7. 2α stimulate differentiation and proliferation, respectively, of clonal osteoblastic MC3T3-E1 cells by different second messengers in vitro. Endocrinology 121:1966-1974.
8. 2 in growing rats: increased metaphyseal hard tissue and cortico-endosteal bone formation. Calcif Tissue Int 37:148-157.
9. 2 enhances cortical bone mass and activates intracortical bone remodeling in intact and ovariectomized female rats. Bone 11:253-266.
10. 2 stimulates the formation of mineralized bone nodules by a cAMP-independent mechanism in the culture of adult rat calvarial osteoblasts. J Cell Biochem 73:36-48.
11. Kato H, Matsuo R, Komiyama O, Tanaka T, Inazu M, Kitagawa H, Yoneda T. 1995. Decreased mitogenic and osteogenic responsiveness of calvarial osteoblasts isolated from aged rats to basic fibroblast growth factor. Gerontology 41:20-27.
12. Lowry OH, Roberts NR, Wu ML, Hixton WS, Crawford EJ. 1954. The quantitative histochemistry of brain. II. Enzyme measurements. J Biol Chem 207:19-37.
13. Nijweide PJ, Burger EH, Feyer JHM. 1986. Cells of bone: proliferation, differentiation and hormonal regulation. Physiol Rev 66:855-886.
14. Norrdin RW, Jee WSS, High WB. 1990. The role of prostaglandins in bone in vivo. Prostaglandins Leukot Essent Fatty Acids 41:139-149.
15. 2 in organ culture, Prostaglandins 8:377-385.
16. Suda M, Tanaka K, Natsui K, Usui T, Tanaka I, Fukushima M, Shigeno C, Konishi J, Narumiya S, Ichikawa A, Nakao K. 1996. Prostaglandin E receptor subtypes in mouse osteoblastic cell line. Endocrinology 137:1698-1705.
17. 2 on bone mass in rat long bones and calvariae. J Endocrinol 156:51-57.
18. 2 in rats. Calcif Tissue Int 52:57-61.