In situ localization and characterization of active proteases in chronically inflamed and healthy human gingival tissues

Journal of Periodontology

Volumn 70, Issue 11, 1999, Pages 1303-1312

  Sarment, David P ^a,b,c,d   Korostoff, Jonathan ^a   D'Angelo, Marina ^e   Polson, Alan M ^e   Feldman, Roy S ^b   Billings, Paul C ^e  

^a UNIVERSITY OF PENNSYLVANIA   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

^c UNIVERSITY OF MICHIGAN SCHOOL OF DENTISTRY   (United States)

^d UNIVERSITY OF MICHIGAN   (United States)

^e NONE

Author keywords

Gingiva enzymology; Inflammation; Matrix metalloproteases; Periodontal diseases diagnosis; Proteases analysis; Serine proteases; Spectroscopy, fluorescence

Indexed keywords

MATRIX METALLOPROTEINASE; PROTEINASE; SERINE PROTEINASE;

ADULT; ARTICLE; ENZYMOLOGY; FLUORESCENCE MICROSCOPY; GINGIVA; GINGIVITIS; HUMAN; METABOLISM; MIDDLE AGED; NONPARAMETRIC TEST; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; TISSUE DISTRIBUTION;

ADULT; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOPEPTIDASES; GINGIVA; GINGIVITIS; HUMANS; HYDROGEN-ION CONCENTRATION; MATRIX METALLOPROTEINASES; MICROSCOPY, FLUORESCENCE; MIDDLE AGED; SERINE ENDOPEPTIDASES; STATISTICS, NONPARAMETRIC; TISSUE DISTRIBUTION;

EID: 0033228697     PISSN: 00223492     EISSN: None     Source Type: Journal    
DOI: 10.1902/jop.1999.70.11.1303     Document Type: Article

References (51)

1. Birkedal-Hansen H. Proteolytic remodeling of extracellular matrix. Curr Opin Cell Biol 1995;7:728-735.
2. Van Der Zee E, Everts V, Beertsen W. Cytokine-induced endogenous procollagenase stored in the extracellular matrix of soft connective tissue results in a burst of collagen breakdown following its activation. J Periodont Res 1996;31:483-488.
3. Schroeder HE, Listgarten MA. The gingival tissues: The architecture of periodontal protection. Periodontol 2000 1997;13:91-120.
4. Bennick A, Hunt AM. Collagenolytic activity in oral tissues. Arch Oral Biol 1967;12:1-9.
5. Fullmer HM, Gibson W. Collagenolytic activity in gingiva of man. Nature 1966;209:728-729.
6. Fullmer HM, Gibson AE, Lazarus GS, Bladen HA. The origin of collagenase in periodontal tissues of man. J Dent Res 1969;48:646-651.
7. Geiger S, Harper E. Human gingival collagenase in periodontal disease: The release of collagenase and the breakdown of endogenous collagen in gingival explants. J Dent Res 1980;59:11-16.
8. Golub LM, Stiegel K, Ramamurthy NS, Mandel ID. Some characteristics of collagenase activity in gingival crevicular fluid and its relationship to gingival diseases in humans. J Dent Res 1976;55:1049-1057.
9. Reynolds JJ, Hembry RM, Meikle MC. Connective tissue degradation in health and periodontal disease and the roles of matrix metalloproteinases and their natural inhibitors. Adv Dent Res 1994;8:312-319.
10. Aiba T, Akeno N, Kawane T, Okamoto H, Horiuchi N. Matrix metalloproteinases-1 and -8 and TIMP-1 mRNA levels in normal and diseased human gingivae. Eur J Oral Sci 1996;104:562-571.
11. Uitto VJ, Airola K, Vaalamo M, et al. Collagenase-3 (matrix metalloproteinase-13) expression is induced in oral mucosal epithelium during chronic inflammation. Am J Pathol 1998;152:1489-1499.
12. Salo T, Lyons JG, Rahemtulla F, Birkedal-Hansen H, Larjava H. Transforming growth factor-beta 1 up-regulates type IV collagenase expression in cultured human keratinocytes. J Biol Chem 1991;266:11436-11441.
13. Sorsa T, Ingman T, Suomalainen K, et al. Identification of proteases from periodontopathogenic bacteria as activators of latent human neutrophil and fibroblast-type interstitial collagenases. Infect Immun 1992;60:4491-4495.
14. Owen CA, Campbell EJ. Neutrophil proteinases and matrix degradation. The cell biology of pericellular proteolysis. Semin Cell Biol 1995;6:367-376.
15. Cooksley S, Hipkiss JB, Tickle SP, et al. Immunoassays for the detection of human collagenase, stromelysin, tissue inhibitor of metalloproteinases (TIMP) and enzyme-inhibitor complexes. Matrix 1990; 10:285-291.
16. Meikle MC, Hembry RM, Holley J, Horton C, McFarlane CG, Reynolds JJ. Immunolocalization of matrix metalloproteinases and TIMP-1 (tissue inhibitor of metalloproteinases) in human gingival tissues from periodontitis patients. J Periodont Res 1994;29: 118-126.
17. Harris ED, Vater CA. Methodology of collagenase research: Substrate preparation, enzyme activation and purification. In: Woolley DE, Evanson JM, eds. Collagenase in Normal and Pathological Connective Tissues. New York: John Wiley and Sons Ltd.; 1980:3.
18. Meitner S, Zander H, Iker H, Poison A. Identification of inflamed gingival surfaces. J Clin Periodontol 1979;6: 93-100.
19. Poison AM, Garrett S, Stoller NH, et al. Multicenter comparative evaluation of subgingivally delivered sanguinarine and doxycycline in the treatment of periodontitis. I. Study design, procedures and managements. J Periodontol 1997;68:110-118.
20. Nyman S, Lindhe J. Examination of patients with periodontal disease. In: Lindhe J, ed. Clinical Periodontology and Implant Dentistry, 3rd ed. Copenhagen: Munksgaard; 1997:383-395.
21. Poison AM, Caton JG, Yeaple RN, Zander AH. Histological determination of probe tip penetration into the gingival sulcus of humans using an electronic pressuresensitive probe. J Clin Periodontol 1980;7:479-488.
22. Greenstein G, Caton J, Poison AM. Histologic characteristics associated with bleeding after probing and visual signs of inflammation. J Periodontol 1981;52: 420-525.
23. Haugland RP. Detecting peptidases and proteases. In: Spence MTZ, ed. Handbook of Fluorescent Probes and Research Chemicals, 6th ed. Eugene, OR: Molecular Probes; 1996:230-232.
24. Birk Y. The Bowman Birk Inhibitor. Int J Peptide Protein Res 1985;25:113-131.
25. Laura R, Robison DJ, Bing DH. (p-Amidinophenly) methanesulfonyl fluoride, an irreversible inhibitor of serine proteases. Biochemist 1980;19:4859-4864.
26. Haerian A, Adonogianaki E, Mooney J, Docherty JP, Kinane DF. Gingival crevicular stromelysin, collagenase and tissue inhibitor of metalloproteinase levels in healthy and diseased sites. J Clin Periodontol 1995;22:505-509.
27. Larivee L, Sodek J, Ferrier JM. Collagenase and collagenase inhibitor activities in crevicular fluid of patients receiving treatment for localized juvenile periodontitis. J Periodont Res 1986;21:702-715.
28. Eley BM, Cox SW. Cathepsin B/L, elastase-, tryptase-, trypsin, and dipeptidyl peptidase IV-like activities in gingival crevicular fluid: Correlation with clinical parameters in untreated chronic periodontitis patients. J Periodont Res 1992;27:62-69.
29. Woolley DE, Davies RM. Immunolocalization of collagenase in periodontal disease. J Periodont Res 1981;16:287-297.
30. Galis ZS, Sukhova GK, Libby P. Microscopic localization of active proteases by in situ zymography: Detection of matrix metalloproteinase activity in vascular tissue. FASEB J 1995;9:974-980.
31. Jones LJ, Upson RH, Haugland RP, et al. Quenched BODIPY Dye-labeled casein substrates for the assay of protease activity by direct fluorescence measurement. Anal Biochem 1997;251:144-152.
32. Ingman T, Sorsa T, Michaelis J, Konttinen YT. Immunohistochemical study of neutrophil-and fibroblast-type collagenases and stromelysin-1 in adult periodontitis. Scand J Dent Res 1994;102:342-349.
33. Ingman T, Sorsa T, Michaelis J, Konttinen YT. Matrix metalloproteinases-1,3,8 in adult periodontitis in situ. Ann NY Acad Sci 1994;732:459-461.
34. Westerlund U, Ingman T, Lukinmaa PL, et al. Human neutrophil gelatinase and associated lipocalin in adult and localized juvenile periodontitis. J Dent Res 1996;75: 1553-1563.
35. Etherington DJ, Pugh D, Silver IA. Collagen degradation in an experimental inflammatory lesion: Studies on the role of the macrophage. Acta Biol Med Ger 1981;40: 1625-1636.
36. Page RC, Schroeder HE. Pathogenesis of inflammatory periodontal disease. A summary of current work. Lab Invest 1976;33:235-249.
37. Kinane DF, Lindhe J. Pathogenesis of periodontitis. In: Lindhe J, ed. Clinical Periodontology and Implant Dentistry, 3rd ed. Copenhagen: Munksgaard; 1997: 194-195.
38. Gross J, Highberger JH, Jonhson-Wint B, Biswas C. Mode of action and regulation of tissue collagenases. In: Woolley DE, Evanson JM, eds. Collagenases in Normal and Pathological Connective Tissues. New York: J. Wiley and Sons Ltd; 1980.
39. Van Der Zee E, Everts V, Beertsen W. Cytokine-induced endogenous procollagenase stored in the extracellular matrix of soft connective tissue results in a burst of collagen breakdown following its activation. J Periodont Res 1996;31:483-488.
40. DeCarlo AA, Windsor LJ, Bodden MK, Harber GJ, Birkedal-Hansen B, Birkedal-Hansen H. Activation and novel processing of matrix metalloproteinases by a thiolproteinase from the oral anaerobe Porphyromonas gingivalis. J DentRes 1997;76:1260-1270.
41. Nomura T, Takahashi T, Hara K. Expression of TIMP-1, TIMP-2 and collagenase mRNA in periodontitis-affected human gingival tissue. J Periodont Res 1993;28: 354-362.
42. Woessner JF. Matrix metalloproteinases and their inhibitors in connective tissue remodeling. FASEB J 1991;5:2145-2154.
43. Reynolds JJ, Hembry RM. Immunolocalization of metalloproteinases and TIMP in normal and pathological tissues. Matrix Suppl 1992;1:375-385.
44. Tervahartiala T, Konttinen YT, lngman T, Hayrinen-Immonen R, Ding Y, Sorsa T. Cathepsin G in gingival tissue and crevicular fluid in adult periodontitis. J Clin Periodontol 1996;23:68-75.
45. Kennett CN, Cox SW, Eley BM. Comparative histochemical, biochemical and immunohistochemical studies of cathepsin B in human gingiva. J Periodont Res 1994;29:203-213.
46. Kunimatsu K, Ozaki Y, Hara Y, Aoki Y, Yamamoto K, Kato I. Immunohistochemical study of cathepsin G and medullasin in inflamed gingival tissues from periodontal patients. J Periodont Res 1997;32:264-270.
47. Birkedal-Hansen H. Role of matrix metalloproteinases in human periodontal diseases. J Periodontol 1993;64: 474-484.
48. Kleinberg I, Hall G. pH and depth of gingival crevices in different areas of the mouth of fasting humans. J Periodont Res 1968;3:109-117.
49. Bicket M, Cimasoni G. The pH of human crevicular fluid measured by a new microanalytical technique. J Periodont Res 1985;20:35-40.
50. Leblebicioglu B, Lim JS, Cario AC, Beck MF, Walters JD. pH changes observed in the inflamed gingival crevice modulate human polymorphonuclear leukocyte activation in vitro. J Periodontol 1996;67:472-477.
51. Korostoff JM, Wang JF, Sarment DP, Feldman R, Billings PC. Increased levels of activated gelatinase A (MMP-2) and serine proteases in chronic adult periodontitis. J Periodontol; in press.