Studies on interdomain interaction of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus, by constructing chimeric enzymes

Extremophiles

Volumn 3, Issue 4, 1999, Pages 259-262

  Numata, Koichi ^a,d   Hayashi Iwasaki, Yoko ^b   Yutani, Katsuhide ^c   Oshima, Tairo ^b  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b TOKYO UNIVERSITY OF PHARMACY AND LIFE SCIENCES   (Japan)

^c OSAKA UNIVERSITY   (Japan)

^d TOYOTA MOTOR CORPORATION   (Japan)

Author keywords

3 lsopropylmalate dehydrogenase; Chimeric proteins; Differential scanning calorimetry; Interdomain interaction; Thermophilic enzymes; Thermostability; Thermus thermophilus

Indexed keywords

3 ISOPROPYLMALATE DEHYDROGENASE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; BACTERIAL GENE; CHIMERA; ENZYME CONFORMATION; ENZYME DENATURATION; ENZYME STABILITY; ENZYME SYNTHESIS; PROTEIN INTERACTION; THERMAL DENATURATION;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); THERMUS THERMOPHILUS;

EID: 0033226828     PISSN: 14310651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s007920050125     Document Type: Article

References (6)

1. Hayashi-Iwasaki Y, Numata K, Yamagishi Y, Yutani K, Sakurai M, Tanaka N, Oshima T (1996) A stable intermediate in the thermal unfolding process of a chimeric 3-isopropylmalate dehydrogenase between a thermophilic and a mesophilic enzymes. Protein Sci 5:511-516
2. Imada K, Sato M, Tanaka N, Katsube Y, Matsuura Y, Oshima T (1991) Three-dimensional structure of a highly thermostable enzyme, 3-isopropylmalate dehydrogenase of Thermus thermophilus at 2.2 Å resolution. J Mol Biol 222:725-738
3. Naumata K, Muro M, Akutsu N, Nosoh Y, Yamagishi A, Oshima T (1995) Thermal stability of chimeric isopropylmalate dehydrogenase genes constructed from a thermophile and a mesophile. Protein Eng 8:39-43
4. Onodera K, Sakurai M, Tanaka N, Numata K, Yamagishi A, Oshima T, Sato M, Katsube Y (1994) Three-dimensional structure of chimeric enzymes between Bacillus subtilis and Thermus thermophilus 3-isopropylmalate dehydrogenases. Protein Eng 7:453-459
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6. Yamada T, Akutsu N, Miyazaki K, Kakinuma K, Yoshida M. Oshima T (1990) Purification, catalytic properties, and thermal stability of threo-Ds-3-isopropylmalate dehydrogenase coded by leuB gene from an extreme thermophile, Thermus thermophilus strain HB8. J Biochem (Tokyo) 108:449-456