Diphtheria toxin forms pores of different sizes depending on its concentration in membranes: Probable relationship to oligomerization

Journal of Membrane Biology

Volumn 171, Issue 3, 1999, Pages 209-221

  Sharpe, J C ^a   London, E ^a  

^a STONY BROOK UNIVERSITY   (United States)

Author keywords

Fluorescence quenching; Membrane translocation; Model membranes

Indexed keywords

CHOLESTEROL; DEXTRAN; DIPHTHERIA TOXIN; OLIGOMER; PHOSPHATIDYLCHOLINE; PHOSPHATIDYLGLYCEROL; PROTEIN; RHODAMINE;

ANTIGEN BINDING; ARTICLE; CHANNEL GATING; CONTROLLED STUDY; CROSS LINKING; FLUORESCENCE; KINETICS; NONHUMAN; OLIGOMERIZATION; PH; STOICHIOMETRY;

DIMERIZATION; DIPHTHERIA TOXIN; FLUORESCENT DYES; MEMBRANES, ARTIFICIAL; ORGANOMETALLIC COMPOUNDS; ORGANOPHOSPHORUS COMPOUNDS; POROSITY;

BACTERIA (MICROORGANISMS);

EID: 0033215457     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002329900572     Document Type: Article

References (45)

1. Bell, C.E., Poon, P.K., Schumaker, V.N., Eisenberg, D. 1997. Oligomerization of a 45 Kilodalton Fragment of Diphtheria Toxin at pH 5.0 to a Molecule of 20-24 Subunits Biochemistry 36:15201-15207
2. Bennett, M.J., Choe, S., Eisenberg, D. 1994. Refined structure of dimeric diphtheria toxin at 2.0 Å resolution. Protein Sci. 3:1444-1463
3. Blewitt, M.G., Chung, L.A., London, E. 1985. Effect of pH on the conformation of diphtheria toxin and its implications for membrane penetration. Biochemistry 24:5458-5464
4. + binding site. Biosci. Reports 5:243-250
5. Carroll, S.F., Barbieri, J.T., Collier, R.J. 1986. Dimeric form of diphtheria toxin: purification and characterization. Biochemistry 25:2425-2430
6. Chattopadhyay, A., London E. 1987. Parallax Method for Direct Measurement of Membrane Penetration Depth Utilizing Fluorescence Quenching by Spin-Labeled Phospholipids. Biochemistry 26;39-45
7. Chen, R.F., Knutsen, J.R. 1988. Mechanism of fluorescence concentration quenching of carboxyfluorescein in liposomes: energy transfer to nonfluorescent dimers. Anal. Biochem. 172: 61-77
8. Choc, S., Bennett, M.J., Fuji, G., Curmi, P.M.G., Kantardjieff, K.A., Collier, R.J., Eisenburg, D. 1992. The crystal structure of diphtheria toxin. Nature 357:216-222
9. Chung, L.A. 1988. Ph.D. Thesis
10. Chung, L.A., London, E. 1988. Interaction of diphtheria toxin with model membranes. Biochemistry 27:1245-1253
11. Collier, R.J. 1982. Structure and activity of diphtheria toxin. In: ADP-Ribosylation Reactions: Biology and Medicine, O. Hayashi, K. Ueda, editors, pp. 573-592. Academic Press, New York
12. Defrise-Quertain, F., Cabiaux, V., Vandenbranden, M., Wattiez, R., Falmagne, P., Ruysschaert, J.-M. 1989. pH Dependent bilayer destabilization and fusion of phosphatidic large unilamellar vesicles induced by diphtheria toxin and its fragment A and fragment B. Biochemistry 28:3406-3413
13. Donovan, J.J., Simon, M.I., Draper, R.K., Montal, M. 1981. Diphtheria toxin forms transmembrane channels in planar lipid bilayers. Proc. Natl. Acad. Sci. USA 78:172-176
14. Falnes, P.O., Madshus, I.H., Sandvig, K., Olsnes, S. 1992. Replacement of negative by positive charges in the presumed membrane-inserted pan of diphtheria toxin B fragment. Effect on membrane translocation and on formation of cation channels. J. Biol. Chem. 267:12284-12290
15. Gordon, V.M., Klimpel, K.R., Arora, N., Henderson, M.A., Leppla, S.M. 1995. Proteolytic activation of bacterial toxins by eukaryotic cells is performed by furin and by additional cellular proteases. Infect. Immun. 63:82-87
16. Henderson, R., Baldwin, J.M., Ceska, T.A., Zemlin, F., Beckmann, H., Downing, K.H. 1990. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy. J. Mol. Biol. 213:899-929
17. Hu, V.W., Holmes, R.K. 1984. Evidence for direct insertion of fragments A and B of diphtheria toxin into model membranes. J. Biol Chem. 259:12226-12233
18. Hudson, T.H., Neville, D.M., Jr. 1985. Quantal entry of diphtheria toxin to the cytosol. J. Biol. Chem. 260:2675-2680
19. Hudson, T.H., Neville, D.M., Jr. 1987, Temporal separation of protein toxin translocation from processing events. J. Biol. Chem. 262:16484-16494
20. Jiang, G.-S., Solow, R., Hu, V. W. 1989. Characterization of Diphtheria Toxin-induced Lesions in Liposomal Membranes. J. Biol. Chem. 264:13424-13429
21. Jiang, J.X., Chung, L.A., London, E. 1991. Self-translocation of diphtheria toxin across model membranes. J. Biol. Chem. 266:24003-24010
22. Kagan, B.L., Finkelstein, A., Colombini, M. 1981. Diphtheria toxin fragments forms large pores in phospholipid bilayer membranes. Proc. Natl. Acad. Sci. USA 78:4950-4954
23. Lanzrein, M., Sand, O., Olsnes, S. 1996. GPI-anchored diphtheria toxin receptor allows membrane translocation of the toxin without delectable ion channel activity. EMBO J. 15:725-734
24. Lanzrein, M., Falnes, P.O., Sand, O., Olsnes, S. 1997. Structure-function relatoinship of the ion channel formed by diphtheria toxin in Vero cell membranes. J. Membrane Biol. 156:141-148
25. London, E. 1992, Diphtheria toxin; membrane interaction and membrane translocation. Biochim. Biophys. Acta 1113:25-51
26. MacDonald, R.I. 1990. Characteristics of self-quenching of the fluorescence of lipid-conjugated rhodamine in membranes. J. Biol. Chem. 265:13533-13539
27. Maliniski, J.A., Nelsestuen, G.L. 1989, Membrane permeability to macromolecules mediated by the membrane attack complex. Biochemistry 28:61-70
28. McKeever, B., Sarma, R. 1982. Preliminary crystallographic investigation of the protein toxin from Corynebacterium diphtheriae. J. Biol. Chem. 257:6923-6925
29. Milne, J.C., Furlong, D., Hanna, P.C., Wall, J.S., Collier, R.J. 1994. Anthrax protective antigen forms oligomers during intoxication of mammalian cells. J. Biol. Chem. 269:20607-20612
30. Mindell, J.A., Silverman, J.A., Collier, R.J., Finkelstein, A. 1994. Structure-function relationships in diphtheria toxin channels: III. Residues which affect the cis pH dependence of channel conductance. J. Membrane Biol. 137:45-57
31. Montecucco, C., Schiavo, G., Tomasi, M. 1985. pH-dependence of the phospholipid interaction of diphtheria-toxin fragments. Biochem. J. 231:123-128
32. Papini, E., Sandona, D., Rappuoli, R., Montecucco, C. 1988. On the membrane translocation of diphtheria toxin: at low pH the toxin induces ion channels on cells. EMBO J. 7:3353-3359
33. Papini E., Schiavo, G., Tomasi, M., Colombatti, M., Rappuoli, R., Montecucco, C. 1987. Lipid interaction of diphtheria toxin and mutants with altered fragment B. 2. Hydrophobic photolabelling and cell intoxication. Eur. J. Biochem. 169:637-644
34. Petosa, C., Collier, R.J., Klimpel, K.R., Leppla, S.H., Liddington, R.C. 1997. Crystal structure of the anthrax toxin protective antigen. Nature 385:833-838
35. Reed, J.C. 1997. Double identity for proteins of the Bcl-2 family. Nature 387:773-778
36. Shiver, J.W., Donovan, J.J. 1987. Interactions of diphtheria toxin with lipid vesicles: determinants of ion-channel formation. Biochim. Biophys. Acta 903:48-55
37. Silverman, J.A., Mindell, J.A., Finkelstein, A., Shen, W.H., Collier, R.J. 1994. Mutational analysis of the helical hairpin region of diphtheria toxin transmembrane domain. J. Biol. Chem. 269:22524-22532
38. Sims, P.J. 1984. Complement protein C9 labeled with fluorescein isothiocyanate can he used to monitor C9 polymerization and formation of the cytolytic membrane lesion, Biochemistry 23:3248-3260
39. Sims, P.J., Wiedmer, T. 1984. Kinetics of polymerization of a fluoresceinated derivative of complement protein C9 by the membrane-bound complex of complement proteins C5b-8. Biochemistry 23:3260-3267
40. Song, L., Hobaugh, M.R., Shustak, C., Cheley, S., Bayley, H., Gouaux, J.E. 1996. Structure of staphylococcal alpha-hemolysin, a heptameric transmembrane pore. Science 274:1859-1866
41. Wang, Y., Pahlo, L., Kachel, K., London, E. 1997. Use of Trp mutations to evaluate the conformational behavior and membrane insertion of A and B chains in whole diphtheria toxin. Biochemistry 36:16300-16308
42. Wang, Y. Malenbaum, S.E., Kachel, K., Zhan, H., Collier, R.J., London, E. 1997. Identification of shallow and deep membrane-penetrating forms of diphtheria toxin T domain that are regulated by protein concentration and bilayer width. J. Biol. Chem. 272:25091-25098
43. Yamaizumi, M., Mekada, E., Uchida, T., Okada, Y. 1978. One molecule of diphtheria toxin fragment A introduced into a cell can kill the cell. Cell 15:245-250
44. Zalman, L.S., Wisnieski, B.J. 1984. Mechanism of insertion of diphtheri toxin: peptide entry and pore size determinations. Proc. Natl. Acad. Sci. USA 81:3341-3395
45. Zhao, J.-M., London, E. 1988. Conformation and model membrane interactions of diphtheria toxin fragment A. J. Biol. Chem. 263:15369-15377