Holyrines A and B, possible intermediates in staurosporine biosynthesis produced in culture by a marine actinomycete obtained from the North Atlantic Ocean

Tetrahedron Letters

Volumn 40, Issue 40, 1999, Pages 7171-7174

  Williams, David E ^a   Bernan, Valerie S ^b   Ritacco, Frank V ^b   Maiese, William M ^b   Greenstein, Michael ^b   Andersen, Raymond J ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

^b PFIZER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HOLYRINE A; HOLYRINE B; INDOLE ALKALOID; MARINE TOXIN; STAUROSPORINE; UNCLASSIFIED DRUG;

ACTINOBACTERIA; ARTICLE; BACTERIUM CULTURE; CYTOTOXICITY; DRUG ISOLATION; DRUG STRUCTURE; NONHUMAN; PARTIAL DRUG SYNTHESIS; SPECTROSCOPY; STRUCTURE ANALYSIS;

EID: 0033215050     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(99)01495-1     Document Type: Article

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7. Microscopic examination of strain N96C-47 revealed a non-fragmented, extensively branched substrate mycelium and abundant yellow-white aerial mycelium which were transformed into sheathed chains of arthrospores. The chains of arthrospores ranged in length from 20 to 30 spores and were classified as rectiflexibiles. Whole cell fatty acid analysis revealed a type 2c fatty acid pattern consisting mainly of saturated iso and anteiso fatty acids. Based on the taxonomic properties described above, and the alignment of the PCR-amplified 16S rRNA genes with the NCBI BLAST database, the strain N96C47 is considered to be a member of the genus Streptomyces. Further studies for species identification are in progress and will be reported elsewhere.
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12. 6 ) δ 172.0 (C-5), 139.1 (C-11a), 138.4 (C-13a), 134.2 (C-7a), 127.1 (C-12a), 125.7 (C-4), 125.3 (C-2), 125.0 (C-10), 123.9 (C-12b), 122.3 (C-4a), 121.7 (C-7c), 121.1 (C-8), 119.8 (C-3,C-9), 118.5 (C-4c), 117.4 (C-4b), 114.9 (C-7b), 111.6 (C-11), 109.4 (C-1), 76.6 (C-2′), 75.0 (C-6′), 66.0 (C-3′), 45.7 (C-4′), 45.1 (C-7), 30.5 (C-5′), 13.8 (C-2′Me).
13. eq), 1.59, s (2′-Me).
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