Rapid uptake and degradation of glycine by astroglial cells in culture: Synthesis and release of serine and lactate

GLIA

Volumn 27, Issue 3, 1999, Pages 239-248

  Verleysdonk, Stephan ^a   Martin, Heiko ^a   Willker, Wieland ^b   Leibfritz, Dieter ^b   Hamprecht, Bernd ^a  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b UNIVERSITY OF BREMEN   (Germany)

Author keywords

Astroglial cells; Glycine; Lactate; Serine

Indexed keywords

CARBON; GLYCINE; LACTIC ACID DERIVATIVE; NITROGEN; SERINE;

ANIMAL; ARTICLE; ASTROCYTE; CELL CULTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; METABOLISM; NEWBORN; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; RAT; TRANSPORT AT THE CELLULAR LEVEL; WISTAR RAT;

ANIMALS; ANIMALS, NEWBORN; ASTROCYTES; BIOLOGICAL TRANSPORT; CARBON ISOTOPES; CARBON RADIOISOTOPES; CELLS, CULTURED; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; GLYCINE; KINETICS; LACTATES; MAGNETIC RESONANCE SPECTROSCOPY; NITROGEN ISOTOPES; RATS; RATS, WISTAR; SERINE;

EID: 0033198805     PISSN: 08941491     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-1136(199909)27:3<239::AID-GLIA5>3.0.CO;2-K     Document Type: Article

References (49)

1. Ando T, Nyhan WL. 1969. The excretion and formation of aminoacetone and delta-aminolevulinic acid in man. Tohoku J Exp Med 99:189-195.
2. Berl S, Nicklas WJ, Clarke DD. 1977. Glial cells and metabolic compartmentation. In: Schoffeniels E, Franck G, Hertz L, Tower DB, editors. Dynamic properties of glia cells. Oxford: Pergamon Press. p 143-149.
3. Bixel MG, Hamprecht B. 1995. Generation of ketone bodies from leucine by cultured astroglial cells. J Neurochem 65:2450-2461.
4. Bixel MG, Dringen R, Wiesinger H, Stock W, Hamprecht B. 1993. Consumption of branched-chain amino acids and glycine in astroglia-rich primary culture. Biol Chem Hoppe-Seyler 374:915.
5. Bommakanti RK, Beavan M, Ng K, Jois M. 1996. Oxidation of glycine by chicken astrocytes in primary culture. J Neurochem 66(Suppl 2):S90A.
6. Bruin WJ, Frantz BM, Sallach HJ. 1973. The occurrence of a glycine cleavage system in mammalian brain. J Neurochem 20:1649-1658.
7. 13C-NMR defines glycine-serine interconversion in situ in the renal proximal tubule. Biochim Biophys Acta 1310:32-40.
8. Daly EC, Aprison MH. 1974. Distribution of serine hydroxymethyl-transferase and glycine transaminase in several areas of the central nervous system of the rat. J Neurochem 22:877-885.
9. Dringen R, Gebhardt R, Hamprecht B. 1993. Glycogen in astrocytes: possible function as lactate supply for neighbouring cells. Brain Res 19:23-30.
10. Dringen R, Hamprecht B. 1996. Glutathione content as an indicator for the presence of metabolic pathways of amino acids in astroglial cultures. J Neurochem 67:1375-1382.
11. Dringen R, Verleysdonk S, Hamprecht B, Willker W, Leibfritz D, Brand A. 1998. Metabolism of glycine in primary astroglial cells: synthesis of creatine, serine and glutathione. J Neurochem 70:835-840.
12. A receptor-mediated chloride fluxes in synaptosomes from different parts of the rat brain. Brain Res 712:74-83.
13. Fubara B, Eckenrode F, Tressel T, Davis L. 1986. Purification and properties of aminoacetone synthetase from beef liver mitochondria. J Biol Chem 261:12189-12196.
14. Fujiwara K, Motokawa Y. 1983. Mechanism of the glycine cleavage reaction. Steady state kinetic studies of the P-protein-catalysed reaction. J Biol Chem 258:8156-8162.
15. Geller AM, Kotb MY. 1989. A binding assay for serine hydroxymethyl-transferase. Anal Biochem 180:120-125.
16. Greferath U, Brandstatter JH, Wässle H, Kirsch J, Kuhse J, Grunert U. 1994. Differential expression of glycine receptor subunits in the retina of the rat: a study using immunohistochemistry and in situ hybridization. Vis Neurosci 11:721-729.
17. Hamberger A, Cotman CW, Sellström A, Weiler CT. 1977. Glutamine, glial cells and their relationship to transmitter glutamate. In: Schoffeniels E, Franck G, Hertz L, Tower DB, editors. Dynamic properties of glia cells. Oxford: Pergamon Press. p 163-172.
18. Hamprecht B, Dringen R. 1995. Energy metabolism. In: Kettenmann H, Ransom B, editors. Neuroglial cells. New York: Oxford University Press. p 473-487.
19. Hamprecht B, Löffler F. 1985. Primary glial cultures as a model for studying hormone action. Methods Enzymol 109:342-345.
20. Hayasaka K, Kochi H, Hiraga K, Kikuchi G. 1980. Purification and properties of glycine decarboxylase, a component of the glycine cleavage system, from rat liver mitochondria and immunochemical comparison of this enzyme from various sources. J Biochem (Tokyo) 88:1193-1199.
21. Hiraga K, Kikuchi G. 1980a. The mitochondrial glycine cleavage system. Purification and properties of glycine decarboxylase from chicken liver mitochondria. J Biol Chem 255:11664-11670.
22. Hiraga K, Kikuchi G. 1980b. The mitochondrial glycine cleavage system. Functional association of glycine decarboxylase and amino-methyl carrier protein. J Biol Chem 255:11671-11676.
23. Holopainen I, Kontro P. 1989. Uptake and release of glycine in cerebellar granule cells and astrocytes in primary culture: potassium-stimulated release from granule cells is calcium-dependent. J Neurosci Res 24:374-383.
24. Ichihara A and Greenberg DM. 1957. Studies on the purification and properties of D-glyceric acid kinase of liver. J Biol Chem 225:949-958.
25. Iwama H, Takahashi K, Kure S, Hayashi F, Narisawa K, Tada K, Mizoguchi M, Takashima S, Tomita U, Nishikawa T. 1997. Depletion of cerebral D-serine in non-ketotic hyperglycinemia: possible involvement of glycine cleavage system in control of endogenous D-serine. Biochem Biophys Res Commun 24:793-796.
26. Johnson JW, Ascher P. 1987. Glycine potentiates the NMDA response in cultured mouse brain neurons. Nature 325:529-531.
27. Jones BN, Gilligan JP. 1983. o-Phthaldialdehyde precolumn derivatisation and reversed-phase high-performance liquid chromatography of polypeptide hydrolysates and physiological fluids. J Chromatogr 266:327-333.
28. Kemp JA, Leeson PD. 1993. The glycine site of the NMDA receptor -five years on. Trends Pharmacol Sci 14:20-25.
29. Kure S, Tada K, Narisawa K. 1997. Nonketotic hyperglycinemia: biochemical, molecular, and neurological aspects. Jpn J Hum Genet 42:13-22.
30. López-Corcuera B, Vázquez J, Aragón C. 1991. Purification of the sodium- and chloride-coupled glycine transporter from central nervous system. J Biol Chem 266:24809-24814.
31. Luque JM, Nelson N, Richards JG. 1995. Cellular expression of glycine transporter 2 messenger RNA exclusively in rat hindbrain and spinal cord. Neuroscience 64:525-535.
32. Miyazaki SS, Toki S, Izumi Y, Yamada H. 1987. Purification and characterization of a serine hydroxymethyltransferase from an obligate methylotroph, Hyphomicrobium methylovorum GM2. Eur J Biochem 162:533-540.
33. Narkewicz MR, Thureen PJ, Sauls SD, Tjoa S, Nikolayevsky N, Fennessey PV. 1996. Serine and glycine metabolism in hepatocytes from mid gestation fetal lamb. Pediatr Res 39:1085-1090.
34. Ogawa H, Fujioka M, Su Y, Kanamoto R, Pitot HC. 1991. Nutritional regulation and tissue-specific expression of the serine dehydratase gene in rat. J Biol Chem 266:20412-20417.
35. Rajendra S, Lynch JW, Schofield PR. 1997. The glycine receptor. Pharmacol Ther 73:121-146.
36. Rampon C, Luppi PH, Fort P, Peyron C, Jouvet M. 1996. Distribution of glycine-immunoreactive cell bodies and fibers in the rat brain. Neuroscience 75:737-755.
37. Rassin DK, Gaull GE. 1975. Subcellular distribution of transmethylation and transsulphuration in rat brain. J Neurochem 24:969-978.
38. Sallach HJ. 1956. Formation of serine from hydroxypyruvate and L-alanine. J Biol Chem 223:1101-1108.
39. Sato K, Yoshida S, Fujiwara K, Tada K, Tohyama M. 1991. Glycine cleavage system in astrocytes. Brain Res 567:64-70.
40. 13C NMR measurements of cerebral glutamine synthesis as evidence for glutamate-glutamine cycling. Proc Natl Acad Sci USA 94:2699-2704.
41. Thompson JS, Richardson KE. 1967. Isolation and characterization of an L-alanine:glyoxylate aminotransferase from human liver. J Biol Chem 242:3614-3619.
42. Thureen PJ, Narkewicz MR, Battaglia FC, Tjoa S, Fennessey PV. 1995. Pathways of serine and glycine metabolism in primary culture of ovine fetal hepatocytes. Pediatr Res 38:775-782.
43. Tsacopoulos M, Magistretti PJ. 1996. Metabolic coupling between glia and neurons. J Neurosci 16:877-885
44. Van den Berg CJ, Matheson DF, Nijemanting WC. 1978. Compartimentation of amino acids in brain: the GABA glutamine-glutamate cycle. In: Fonnum F, editor. Amino acids and chemical transmitters. New York: Plenum Press. p 709-723.
45. Westergaard N, Sonnewald U, Schousboe A. 1995. Metabolic trafficking between neurons and astrocytes: the glutamate/glutamine cycle revisited. Dev Neurosci 17:203-211.
46. Willis JE, Sallach HJ. 1962. Evidence for a mammalian D-glyceric dehydrogenase. J Biol Chem 237:910-915.
47. 13C-labeled biofluids. J Magn Res 125:216-219.
48. Yudkoff M, Daikhin Y, Nissim I, Pleasure D, Stern J, Nissim I. 1994. Inhibition of astrocyte glutamine production by α-ketoisocaproic acid. J Neurochem 63:1508-1515.
49. Zafra F, Aragon C, Olivares L, Danbolt NC, Gimenez C, Storm-Mathisen J. 1995. Glycine transporters are differentially expressed among CNS cells. J Neurosci 15:3952-3969.