Efficient production of recombinant human erythropoietin by replenishment of microcarriers in the hollow fiber culture cassette

Bioscience, Biotechnology and Biochemistry

Volumn 63, Issue 9, 1999, Pages 1624-1626

  Inoue, Yuichi ^a   Arita, Nobuhisa ^b   Teruya, Kiichiro ^b   Katakura, Yoshinori ^b   Shirahata, Sanetaka ^b  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

Author keywords

BHK 21 cells; Hollow fiber bioreactor; Human erythropoietin; Microcarrier; Ras oncogene

Indexed keywords

RECOMBINANT ERYTHROPOIETIN;

ANIMAL; ARTICLE; BIOREACTOR; BIOTECHNOLOGY; CELL LINE; CULTURE MEDIUM; HAMSTER; HUMAN; KINETICS; METABOLISM;

ANIMALS; BIOREACTORS; BIOTECHNOLOGY; CELL LINE; CRICETINAE; CULTURE MEDIA; ERYTHROPOIETIN, RECOMBINANT; HUMANS; KINETICS;

EID: 0033195663     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.63.1624     Document Type: Article

References (9)

1. Yano, T., Teruya, K., Shirahata, S., Watanabe, J., Osada, K., Tachibana, H., Ohashi, H., Kim, E.-H., and Murakami, H., Ras oncogene enhances the production of a recombinant protein regulated by the cytomegalovirus promoter in BHK-21 cells. Cytotechnology, 16, 167-178 (1994).
2. Shoji, M., Kawamoto, S., Seki, K., Teruya, Y., Setoguchi, Y., Mochizuki, K., Kato, M., Hashizume, S., Hanagiri, T., Yoshimatsu, T., Nakanishi, K., Yasumoto, K., Nagashima, A., Nakahashi, H., Suzuki, T., Imai, T., Shirahata, S., Nomoto, K., and Murakami, H., Lung cancer-reacting human recombinant antibody AE6F4: potential usefulness in the sputum cytodiagnosis. Hum. Antibod. Hybridoma, 7, 27-36 (1996).
3. Inoue, Y., Kawamoto, S., Seki, K., Teruya, K., Mochizuki, K., Kato, M., Hashizume, S., Yasumoto, K., Nagashima, A., Nakahashi, H., Suzuki, T., Imai, T., Nomoto, K., and Shirahata, S., Production of a recombinant human monoclonal antibody using a novel hollow fiber bioreactor system. J. Ferment. Bioeng., 81, 466-469 (1996).
4. Shoji, M., Kawamoto, S., Setoguchi, Y., Mochizuki, K., Honjoh, T., Kato, M., Hashizume, S., Hanagiri, T., Yoshimatsu, T., Nakanishi, K., Yasumoto, K., Nagashima, A., Nakanishi, H., Suzuki, T., Imai, T., Nomoto, K., and Murakami, H., The 14-3-3 protein as the antigen for lung cancer-associated human monoclonal antibody AE6F4. Hum. Antibod. Hybridoma, 5, 123-130 (1994).
5. Inoue, N., Takeuchi, M., Ohashi, H., and Suzuki, T., The production of recombinant human erythropoietin. Biotech. Annu. Rev., 1, 297-313 (1995).
6. Powell, J. S., Berkner, K. L., Lebo, R. V., and Adamson, J. W., Human erythropoietin gene: High level expression in stable transfected mammalian cells and chromosome localization. Proc. Natl. Acad. Sei. USA, 83, 6465-6469 (1986).
7. Mori, T., Yamamoto, K., Ohta, T., Sakamoto, C., Sato, M., Koide, K., Murakami, T., Fijii, M., Fukuda, S., and Kurimoto, M., A high-level and regulatable production system for recombinant glycoproteins using a human interferon-a promoter-based expression vector. Gene, 144, 289-293 (1994).
8. Cazlaris, H., Le Marer, N., Laudet, V., Lagrou, C., Zhu, Q., Delannoy, P., and Montreuil, J., Modifications of sialylation in BHK 21/03 cells after in vitro transfection by c-Ha-ras human oncogene. C. R. Acad. Sei. III., 312, 293-300 (1991).
9. Takagi, M., Shirokaze, J., Oishi, K., Otsubo, K., Yamamoto, K., Yoshida, N., and Fujimatsu, I., Production of S —(+)-Ibuprofen with high optical purity from a nitrile compound by cells immobilized on cellulose porous beads. J. Ferment. Bioeng., 78, 191-193 (1994).