A radiochemical assay for β-ureidopropionase using radiolabeled N- carbamyl-β-alanine obtained via hydrolysis of [2-14C]5,6-dihydrouracil

Analytical Biochemistry

Volumn 272, Issue 2, 1999, Pages 250-253

  Van Kuilenburg, A B P ^a   Van Lenthe, H ^a   Van Gennip, A H ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

5 fluorouracil; Dihydrouracil; Pyrimidine degradation; Ureidopropionase; alanine; alanine synthase

Indexed keywords

HYDROLYSIS;

5-FLUOROURACIL; COEFFICIENT OF VARIATION; DIHYDROURACIL; LIQUID SCINTILLATION COUNTING; MICHAELIS-MENTEN KINETIC; PROTEIN CONCENTRATIONS; SYNTHASES; UREIDOPROPIONASE;

AMINO ACIDS;

BETA ALANINE; BETA UREIDOPROPIONASE; DIHYDROPYRIMIDINE DEHYDROGENASE; DIHYDROURACIL; FLUOROURACIL; N CARBAMYL BETA ALANINE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ASSAY; ENZYME KINETICS; ENZYME MECHANISM; HUMAN; HUMAN TISSUE; HYDROLYSIS; ISOTOPE LABELING; LIQUID SCINTILLATION COUNTING; LIVER HOMOGENATE; MICHAELIS MENTEN KINETICS; PRIORITY JOURNAL;

EID: 0033180462     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1999.4181     Document Type: Article

References (23)

1. Van Gennip A. H., Abeling N. G. G. M., Vreken P., Van Kuilenburg A. B. P. J. Inher. Metab. Dis. 20:1997;203-213.
2. Van Gennip A. H., Abeling N. G. G. M., Vreken P., Van Kuilenburg A. B. P. Int. Pediatr. 12:1997;28-33.
3. Van Kuilenburg A. B. P., Vreken P., Abeling N. G. G. M., Bakker H. D., Meinsma R., Van Lenthe H., De Abreu R. A., Smeitink J. A. M., Kayserili H., Apak M. Y., Christensen E., Holopainen I., Pulkki K., Riva D., Botteon G., Holme E., Tulinius M., Kleijer W. J., Beemer F. A., Duran M., Niezen-Koning K. E., Smit G. P. A., Jakobs C., Smit L. M. E., Moog U., Spaapen L. J. M., Van Gennip A. H. Hum. Genet. 104:1999;1-9.
4. Assmann B., Göhlich-Ratmann G., Bräutigam C., Wagner L., Moolenaar S., Engelke U., Wevers R., Voit T., Hoffmann G. F. J. Inher. Metab. Dis. 21:1998;1.
5. Tamaki N., Mizutani N., Kikugawa M., Fujimoto S., Mizota C. Eur. J. Biochem. 169:1987;21-26.
6. Campbell L. L. J. Biol. Chem. 235:1960;2375-2378.
7. Traut T. W., Loechel S. Biochemistry. 23:1984;2533-2539.
8. Waldmann G., Podschun B. Anal. Biochem. 188:1990;233-236.
9. Lengfeld F., Stieglitz J. Am. Chem. J. 15:1883;504-518.
10. Nyc J. F., Mitchell H. K. J. Am. Chem. Soc. 69:1947;1382-1384.
11. Fritzson P. J. Biol. Chem. 226:1957;223-228.
12. Hardiman M. K., Alfant M., Wakelin V. P., Tremblay G. C. Arch. Biochem. Biophys. 224:1983;326-331.
13. Matthews M. M., Traut T. W. J. Biol. Chem. 262:1987;7232-7237.
14. Van Kuilenburg A. B. P., Van Lenthe H., Van Gennip A. H. Anticancer Res. 16:1996;389-394.
15. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C. Anal. Biochem. 150:1985;76-85.
16. Cornisch-Bowden A., Eisenthal R. Biochim. Biophys. Acta. 523:1978;268-272.
17. Fink R. M., McGaughey C., Cline R. E., Fink K. J. Biol. Chem. 218:1956;1-7.
18. Canellakis E. S. J. Biol. Chem. 221:1956;315-322.
19. Fritzson P., Pihl A. J. Biol. Chem. 226:1957;229-235.
20. Matthews M. M., Liao W., Kvalnes-Krick K. L., Traut T. W. Arch. Biochem. Biophys. 293:1992;254-263.
21. Van Gennip A. H., De Abreu R. A., Van Lenthe H., Bakkeren J., Rotteveel J., Vreken P., Van Kuilenburg A. B. P. J. Inher. Metab. Dis. 20:1997;339-342.
22. Van Gennip A. H., Abeling N. G., Elzinga-Zoetekouw L., Scholten L. G., Van Cruchten A., Bakker H. D. Adv. Exp. Med. Biol. 253A:1989;111-118.
23. Van Gennip A. H., Busch S., Elzinga L., Stroomer A. E. M., Van Cruchten A., Scholten E. G., Abeling N. G. G. M. Clin. Chem. 39:1993;380-385.