Structure and function of facilitative sugar transporters

Current Opinion in Cell Biology

Volumn 11, Issue 4, 1999, Pages 496-502

  Barrett, Michael P ^a   Walmsley, Adrian R ^a   Gould, Gwyn W ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; GLUCOSE TRANSPORTER; LACTOSE PERMEASE; SUGAR;

CELL MEMBRANE TRANSPORT; CRYSTAL STRUCTURE; ESCHERICHIA COLI; KINETICS; MUTAGENESIS; PRIORITY JOURNAL; REVIEW; STRUCTURE ACTIVITY RELATION; SUGAR TRANSPORT; YEAST;

EID: 0033179853     PISSN: 09550674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0955-0674(99)80072-6     Document Type: Article

References (38)

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6. + symporters by mutations and construction of chimeras. J Biol Chem 1998, 273:11456-11462. A recent paper on the Chlorella transporters. The introduction provides a good general background. Selection of mutants of these transporters expressed in S. pombe deficient in deoxyglucose uptake allowed identification of residues involved in exofacial binding. Site-directed mutagenesis experiments pinpointed the residues involved in D-galactose selectivity in one of the HUP transporters.
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38. Zhuang J, Prive GG, Werner GE, Ringler P, Kaback HR, Engel A: Two dimensional crystallisation of Escherichia coli lactose permease. J Struct Biol 1999, 125:63-75. This paper describes the production and analysis of two dimensional crystals of the lactose permease. The methodology outlined will be of great interest to many workers attempting to produce crystals of membrane proteins, and should provide further impetus towards the production of a detailed structure for an MF family member.