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Volumn 9, Issue 4, 1999, Pages 438-443

Lipid monolayers: Why use half a membrane to characterize protein-membrane interactions?

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE LIPID; MEMBRANE PROTEIN; WATER;

EID: 0033179620     PISSN: 0959440X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-440X(99)80061-X     Document Type: Article
Times cited : (490)

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    • in press. Near-field scanning optical microscopy provides data similar to that from epifluorescence imaging, but with resolution of the domain structure on the submicron scale. This paper reports the extension of the technique from supported monolayers (see [55]) to spread monolayers at the air-2M sucrose interface. Importantly, the results show that domain substructures, observed in lipid monolayers transferred onto a solid support and imaged in air [55], are absent when the monolayer is imaged in situ. Moreover, data are presented showing that true imaging at the air-water interface should be possible if an interferometric feedback method is used to control the tip height and perturbation of the lipid monolayer by the tip
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