Glutathione levels in primary glial cultures: Monochlorobimane provides evidence of cell type-specific distribution

GLIA

Volumn 27, Issue 2, 1999, Pages 152-161

  Chatterjee, Shampa ^a   Noack, Heiko ^a   Possel, Heiko ^a   Keilhoff, Gerburg ^a   Wolf, Gerald ^a  

^a OTTO VON GUERICKE UNIVERSITY   (Germany)

Author keywords

Astrocytes; Fluorescence microscopy; mBCl GSH conjugate; Microgila; Monochlorobimane

Indexed keywords

BIOLOGICAL MARKER; FIXATIVE; FLUORESCENT DYE; FORMALDEHYDE; GLUTARALDEHYDE; GLUTATHIONE; MONOCHLOROBIMANE; PARAFORMALDEHYDE; POLYMER; PYRAZOLE DERIVATIVE;

ANIMAL; ARTICLE; CELL CULTURE; CHEMISTRY; CONFOCAL MICROSCOPY; FLUORESCENCE MICROSCOPY; GLIA; MICROGLIA; NEWBORN; RAT; TISSUE FIXATION; WISTAR RAT;

ANIMALS; ANIMALS, NEWBORN; BIOLOGICAL MARKERS; CELLS, CULTURED; FIXATIVES; FLUORESCENT DYES; FORMALDEHYDE; GLUTARAL; GLUTATHIONE; MICROGLIA; MICROSCOPY, CONFOCAL; MICROSCOPY, FLUORESCENCE; NEUROGLIA; POLYMERS; PYRAZOLES; RATS; RATS, WISTAR; TISSUE FIXATION;

EID: 0033179501     PISSN: 08941491     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1098-1136(199908)27:2<152::AID-GLIA5>3.0.CO;2-Q     Document Type: Article

References (46)

1. Anderson ME. 1998. Glutathione: an overview of biosynthesis and modulation. Chem Biol Interact 111-112:1-14.
2. Arrick BA, Nathan CF. 1984. Glutathione metabolism as a determinant of therapeutic efficiency. A review. Cancer Res 44:4224-4232.
3. Baker M, Cerniglia GJ, Zaman A. 1990. Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples. Anal Biochem 190:360-365.
4. Barhoumi R, Bailey RH, Burghardt RC. 1995. Kinetic analysis of glutathione in anchored cells with monochlorobimane. Cytometry 19:226-234.
5. Beckman JS, Koppenol WH. 1996. Nitric oxide, superoxide and peroxynitrite: the good, the bad and the ugly. Am J Physiol 271:C1424-C1437.
6. Beiswanger CM, Diegmann MH, Novak RF, Philbert MA, Graessle TL, Reuhl KR, Lowndes HE. 1995. Developmental changes in the cellular distribution of glutathione and glutathione-S-transferases in the murine nervous system. Neurotoxicology 16:425-440.
7. Bellomo G, Palladini G, Vairetti M. 1997. Internuclear distribution, function and fate of glutathione and glutathione-S-conjugate in living rat hepatocytes studied by fluorescence microscopy. Microsc Res Tech 36:243-252.
8. Boje KM. 1997. The role of glial nitric oxide in neurotoxicity and central nervous system diseases. In: Wood PL, editor. Neuroinflammation: mechanisms and management. Englewood Cliffs, NJ: Humana Press Inc. p 331-354.
9. Briviba K, Fraser G, Sies H, Ketterer B. 1993. Distribution of monochlorobimane-glutathione conjugate between nucleus and cytosol in isolated hepatocytes. Biochem J 294:631-633.
10. Calka J, Wolf G, Schmidt W. 1996. Induction of cytosolic NADPH-diaphorase/nitric oxide synthase in reactive microglia/macrophages after quinolinic acid lesions in the rat striatum: an electron and light microscopic study. Histochem Cell Biol 105:81-89.
11. Coates A, Tripp E. 1995. Comparison of two fluorochromes for flow cytometric assay of cellular glutathione content in human malignant melanoma. Melanoma Res 5:107-111.
12. Cook JA, Iype SN, Mitchell JB. 1991. Differential specificity of monochlorobimane for isozymes of human and rodent glutathione-S-transferases. Cancer Res 51:1606-1612.
13. Cooper AJL. 1997. Glutathione in the brain: disorders of glutathione metabolism. In: Rosenberg RN, Prusiner SB, DiMauro S, Barchi RL, Kunk LM, editors. The molecular and genetic basis of neurological disease. Boston: Butterworth-Heinemann. p 1195-1230.
14. Cooper AJL, Kristal BS. 1997. Multiple roles of glutathione in the central nervous system. Biol Chem 378:793-802.
15. Devesa A, O'Connor JE, Garcia C, Puertes IR, Vina, JR. 1993. Glutathione metabolism in primary astrocyte cultures: flow cytometric evidence of heterogeneous distribution of GSH content. Brain Res 618:181-189.
16. Dringen R, Hamprecht B. 1997. Involvement of glutathione peroxidase and catalase in the disposal of exogenous hydrogen peroxide by cultured astroglial cells. Brain Res 759:67-75.
17. Dringen R, Hamprecht B. 1998. Glutathione restoration as indicator for cellular metabolism of astroglial cells. Dev Neurosci 20:401-407.
18. Fernandez-Checa JC, Kaplowitz N. 1990. The use of monochlorobimane to determine hepatic GSH levels and synthesis. Anal Biochem 190:212-219.
19. Flohe L. 1982. Glutathione peroxidase brought into focus. Free Rad Biol Med 5:223-254.
20. Galea E, Feinstein DL, Reis DJ. 1992. Induction of calcium-independent nitric oxide synthase activity in primary glial cultures. Proc Natl Acad Sci USA 89:10945-10949.
21. Galea E, Reis DJ, Feinstein DL. 1994. Cloning and expression of inducible nitric oxide synthase from rat astrocytes. J Neurosci Res 37:406-414.
22. Griffith OW. 1980. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 106:207-212.
23. Haughland RP. 1992. Handbook of fluorescent probes and research chemicals. Eugene, OR: Molecular Probes, Inc.
24. Hedley DW, Chow S. 1994. Evaluation of methods for measuring cellular glutathione content using flow cytometry. Cytometry 15:349-358.
25. Herzenberg LA, DeRosa SC, Dubs JG, Roederer M, Anderson MT, Ela SW, Deresinski SC, Herzenberg LA. 1997. Glutathione deficiency is associated with impaired survival in HIV disease. Proc Natl Acad Sci USA 94:1967-1972.
26. Hothersall JS, Cunha FQ, Neild GH, Noronha-Dutra AA. 1997. Induction of nitric oxide synthesis in J774 cells lowers intracellular glutathione: effect of modulated glutathione redox status on nitric oxide synthase induction. Biochem J 322:477-481.
27. Huang J, Philbert MA. 1995. Distribution of glutathione and glutathi-one-related enzyme systems in mitochondria and cytosol of cultured cerebeller astrocytes and granule cells. Brain Res 680:16-22.
28. Klegeris A, McGeer PL. 1994. Rat brain microglia and peritoneal macrophages show similar responses to respiratory burst stimulants. J Neuroimmunol 53:83-90.
29. Kosower NS, Kosower EM. 1995. Bromobimane probes for thiols. Methods Enzymol 251:133-148.
30. Larrauri A, Lopez P, Gomez-Lechon MJ, Castell JV. 1987. A cytochemical stain for glutathione in rat hepatocytes cultured on plastic. J Histochem Cytochem 35:271-274.
31. Meister A. 1994. Glutathione, ascorbate and cellular protection. Cancer Res (Suppl) 54:1969s-1975s.
32. Millis KK, Lesko SA, Gamesik MP. 1997. Formation, intracellular distribution and efflux of glutathione-bimane conjugates in drug-sensitive and -resistant MCF-7 cells. Cancer Chemother Pharmacol 40:101-111.
33. Philbert MA, Beiswanger CM, Waters DK, Reuhl KR, Lowndes HE. 1991. Cellular and regional distribution of reduced glutathione in the nervuous system of rat: histochemical localisation by mercury orange and o-phthaldialdehyde induced histofluorescence. Toxicol Appl Pharmacol 107:215-227.
34. Poot M, Kavanagh TJ, Kang HC, Haughland RP, Rabinovitch PS. 1991. Flow cytometric analysis of cell cycle-dependent changes in cell thiol level by combining a new laser dye with Hoechst 33342. Cytometry 12:184-187.
35. Pulaski L, Bartosz G. 1995. Transport of bimane-S-glutathione in human erythrocytes. Biochim Biophys Acta 1268:279-284.
36. Radi R, Beckman JS, Bush KM, Freeman BA. 1991. Peroxynitrite oxidation of sulfhydrylg. J Biol Chem 266:4244-4250.
37. Raps SP, Lai JCK, Hertz L, Cooper AJL. 1989. Glutathione is present in high concentrations in cultured astrocytes but not in cultured neurons. Brain Res 493:398-401.
38. Rice ME, Russo-Menna I. 1998. Differential compartmentalization of brain ascorbate and glutathione between neurons and glia. Neuroscience 82:1213-1223.
39. Sagara J, Miura K, Bannai S. 1993. Maintenace of neuronal glutathione by glial cells. J Neurochem 61:1672-1676.
40. Schousboe A. 1980. Primary cultures of astrocytes from mammalian brain as a tool in neurochemical research. Cell Mol Biol 26:505-513.
41. Sies H, Sharov VS, Klotz LO, Briviba K. 1997. Glutathione peroxidase protects against peroxynitrite-mediated oxidations. A new function for selenoproteins as peroxynitrite reductase. J Biol Chem 272: 27812-27817.
42. Simmons ML, Murphy S. 1992. Induction of nitric oxide synthase in glial cells. J Neurochem 59:897-905.
43. Thomas M, Nicklee T, Hedley DW. 1995. Differential effects of depleting agents on cytoplasmic and nuclear non-protein sulphydryls: a fluorescence image cytometry study. Br J Cancer 72:45-50.
44. Thorburne SK, Juurlink H. 1996. Low glutathione and high iron govern the susceptibility of oligodendroglial precursors to oxidative stress. J Neurochem 67:1014-1022.
45. Tietze F. 1969. Enzymatic method for quantitative determination of nanogram amounts of total and oxidised glutathione: application to mammalian blood and other tissues. Anal Biochem 27:502-522.
46. Ublacker GA, Johnson JA, Siegel FL, Mulcahy RT. 1991. Influence of glutathione-S-transferases on cellular glutathione determination by flow cytometry using monochlorobimane. Cancer Res 51:1783-1788.