An unusual case of 'uncompetitive activation' by ascorbic acid: Purification and kinetic properties of a myrosinase from Raphanus sativus seedlings

Biochemical Journal

Volumn 341, Issue 3, 1999, Pages 725-732

  Shikita, Mikio ^a   Fahey, Jed W ^a   Golden, Tamara R ^a   Holtzclaw, W David ^a   Talalay, Paul ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

thioglucoside; Glucohydrolase; Glucosinolate; Isothiocyanate; Sinigrin

Indexed keywords

ASCORBIC ACID; GLUCOSIDASE; GLYCEROL; SINIGRIN; SULFATE; THIOGLUCOSIDASE; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; CHROMATOGRAPHY; COMPETITIVE INHIBITION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME SUBUNIT; GEL FILTRATION CHROMATOGRAPHY; HYDROLYSIS; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL;

ASCORBIC ACID; BRASSICACEAE; CHROMATOGRAPHY, ION EXCHANGE; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME ACTIVATION; GLYCOSIDE HYDROLASES; KINETICS;

RAPHANUS SATIVUS; RAPHANUS SATIVUS VAR. NIGER;

EID: 0033178510     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3410725     Document Type: Article

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