A rapid protocol for the authentication of isolated differential display RT-PCR CDNAs

Preparative Biochemistry and Biotechnology

Volumn 29, Issue 3, 1999, Pages 245-255

  Miele, Gino ^a,b   Slee, Roger ^c   Manson, Jean ^b   Clinton, Michael ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b BBSRC Neuropathogensis Unit   (United Kingdom)

^c WESTERN GENERAL HOSPITAL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

CDNA LIBRARY; OLIGONUCLEOTIDE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING;

EID: 0033178490     PISSN: 10826068     EISSN: None     Source Type: Journal    
DOI: 10.1080/10826069908544927     Document Type: Article

References (9)

1. D. Callard, B. Lescure, L. Mazzolini, "A Method for the Elimination of False Positives Generated by the Mrna Differential Display Technique," Techniques, 16, 1096-1103 (1994).
2. F. Li, E. S. Bamathan, K. Kariko, "Rapid Method for Screening and Cloning of cDNAs Generated in Differential Messenger-RNA Display and Application of Northern Blot for Affinity Capture of cDNAs," Nucleic Acids Res., 22, 1764-1765 (1994).
3. P. Liang, A. B. Pardee, "Differential Display of Eukaryotic Messenger RNA by Means of the Polymerase Chain Reaction," Science, 257, 967-971 (1992).
4. C. Lui, K. Raghothama, "Practical Method for Cloning cDNAs Generated in an mRNA Differential Display," BioTechniques, 20, 576-580 (1996).
5. G. Miele, L. MacRae, D. McBride, J. Manson, M. Clinton, "Elimination of False-Positives Generated Through PCR Re-amplification of Differential Display cDNA," BioTechniques, 25, 138-144 (1998).
6. L. Mou, H. Miller, J. Li, E. Wang, L. Chalifour, "Improvements to the Differential Display Method for Gene Analysis," Biochem. Biophys. Res. Comm., 199, 564-569 (1994).
7. G. Poirier, M.-C., J. Payati, J. S. Wan, M. G. Erlander "Screening of Differentially Expressed cDNA Clones Obtained by Differential Display using Amplified RNA," Nucleic Acids Res., 25, 913-914 (1997).
8. J. Sambrook, E. F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual, 2nd ed, CSH Laboratory Press, Cold Spring Harbor, NY (1989).
9. Y. Sun, G. Hegamyer, N. H. Colburn, "Molecular Cloning of 5 Messenger RNAs Differentially Expressed in Preneoplastic or Neoplastic JB6 Mouse Epidermal Cells - One of Which is Homologous to Human Tissue Inhibitor of Metalloproteinase 3," Cancer Res., 54, 1139-1144 (1994).