Elimination of false positives generated through PCR re-amplification of differential display cDNA

BioTechniques

Volumn 25, Issue 1, 1998, Pages 138-144

  Miele, G ^a,b   MacRae, L ^a   McBride, D ^a   Manson, J ^b   Clinton, Michael ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b BBSRC/MRC Neuropathogenesis Unit   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; GENE AMPLIFICATION; LABORATORY DIAGNOSIS; MOLECULAR CLONING; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; SINGLE STRAND CONFORMATION POLYMORPHISM;

EID: 0031868919     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/98251rr02     Document Type: Article

References (10)

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2. Callard, D., B. Lescure and L. Mazzolini. 1994. A method for the elimination of false positives generated by the mRNA differential display technique. BioTechniques 16:1096-1103.
3. Hayashi, K. 1991. PCR-SSCP: a simple and sensitive method for detection of mutations in genomic DNA. PCR Methods Appl. 1:34-38.
4. Li, F., E.S. Barnathan and K. Kariko. 1994. Rapid method for screening and cloning cDNAs generated in differential messenger-RNA display and application of northern blot for affinity capture of cDNAs. Nucleic Acids Res. 22:1764-1765.
5. Liang, P. and A.B. Pardee. 1992. Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction. Science 257:967-971.
6. Lui, C. and K.G. Raghothama. 1996. Practical method for cloning cDNAs generated in an mRNA differential display. BioTechniques 20:576-580.
7. Mathieu-Daude, F., R. Cheng, J. Welsh and M. McClelland. 1996. Screening of differentially amplified cDNA products from RNA arbitrarily primed PCR fingerprints using single-strand conformation polymorphism (SSCP) gels. Nucleic Acids Res. 24:1504-1507.
8. Mathieu-Daude, F., J. Welsh, T. Vogt and M. McClelland. 1996. DNA rehybridization during PCR - the C(o)T effect and its consequences. Nucleic Acids Res. 24:2080-2086.
9. Mou, L., H. Miller, J. Li, E. Wang and L. Chalifour. 1994. Improvements to the differential display method for gene analysis. Biochem. Biophys. Res. Commun. 199:564-569.
10. Sun, Y., G. Hegamyer and N.H. Colburn. 1994. Molecular cloning of 5 messenger RNAs differentially expressed in preneoplastic or neoplastic JB6 mouse epidermal cells - one which is homologous to human tissue inhibitor of metalloproteinase 3. Cancer Res. 54:1139-1144.