Green-Tissue-specific expression of a reconstructed cry1c gene encoding the active fragment of bacillus thuringiensis δ-endotoxin in haploid tobacco plants conferring resistance to spodopter

Bioscience, Biotechnology and Biochemistry

Volumn 63, Issue 8, 1999, Pages 1433-1444

  Christov, Nikolai Kirilov ^a,b   Imaishi, Hiromasa ^a   Ohkawa, Hideo ^a  

^a KOBE UNIVERSITY   (Japan)

^b Institute of Genetic Engineering   (Bulgaria)

Author keywords

Bacillus thuringiensis; Cry1C; Insect resistance; Spodoptera; Transgenic plants

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN; CRYPTOCHROME; CRYPTOCHROME PROTEIN, DROSOPHILA; CYTOCHROME P450 1A1; DROSOPHILA PROTEIN; ENDOTOXIN; EYE PROTEIN; FLAVOPROTEIN; G PROTEIN COUPLED RECEPTOR; HEMOLYSIN; INSECTICIDAL CRYSTAL PROTEIN, BACILLUS THURINGIENSIS; ISOPROTEIN;

AMINO ACID SEQUENCE; ANIMAL; ARMYWORM; ARTICLE; BIOLOGICAL PEST CONTROL; CHEMISTRY; DRUG RESISTANCE; GENETIC CODE; GENETIC TRANSFORMATION; GENETICS; HAPLOIDY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PATHOGENICITY; PHOTORECEPTOR; PLANT; RAT; TOBACCO; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; ANIMALS; BACTERIAL PROTEINS; BACTERIAL TOXINS; BASE SEQUENCE; CYTOCHROME P-450 CYP1A1; DROSOPHILA PROTEINS; DRUG RESISTANCE; ENDOTOXINS; EYE PROTEINS; FLAVOPROTEINS; GENETIC CODE; HAPLOIDY; HEMOLYSIN PROTEINS; MOLECULAR SEQUENCE DATA; PEST CONTROL, BIOLOGICAL; PHOTORECEPTORS, INVERTEBRATE; PLANTS, GENETICALLY MODIFIED; PLANTS, TOXIC; PROTEIN ISOFORMS; RATS; RECEPTORS, G-PROTEIN-COUPLED; SPODOPTERA; TOBACCO; TRANSFORMATION, GENETIC;

EID: 0033174906     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.63.1433     Document Type: Article

References (38)

1. Crickmore, N., Zeigler, D. R., Feitelson, J., Schnepf, E., Van Rie, J., Lereclus, D., Baum, J., and Dean, D. H., Revision of the nomenclature for the Bacillus thuringiensis pesticidal crystal proteins. Microbiol. Mol. Biol. Rev., 62, 807-813 (1998).
2. Fischhoff, D. A., Bowdish, K. S., Perlak, F. J., Marrone, P. G., McCormick, S. M., Niedermeyer, J. G., Dean, D. A., Kusano-Kretzmer, K., Mayer, E. J., Rochester, D. E., Rogers, S. G., and Fraley, R. T., Insect tolerant transgenic tomato plants. Biotechnology (N.Y.), 5, 807-813 (1987).
3. Perlak, F. J., Deaton, R. W., Armstrong, T. A., Fuchs, R. L., Sims, S. R., Greenplate, J. T., and Fischhoff, D. A., Insect resistant cotton plants. Biotechnology (N. Y.), 8, 939-943 (1990).
4. Fujimoto, H., Itoh, K., Yamamoto, M., Kyozuka, J., and Shimamoto, K., Insect resistant rice generated by introduction of a modified deltα-endotoxin gene of Bacillus thuringiensis. Biotechnology (N.Y.), 11, 1111-1115 (1993).
5. Barton, K. A., Whiteley, H. R., and Yang, N. S., Bacillus thuringiensis 5-endotoxin expressed in transgenic Nicotiana tabacum provides resistance to lepidopteran insects. Plant Physiol., 85, 1103-1109 (1987).
6. Vaeck, M., Reynaerts, A., Hofte, H., Jansens, S., De Beuskeleer, M., Dean, C., Zabeau, M., van Montagu, M., and Leemans, J., Transgenic plants protected from insect attack. Nature, 328, 33-37 (1987).
7. Adang, M. J., Firoozabady, E., Klein, J., DeBoer, D., Sekar, V., Kemp, J. D., Murray, E. E., Rocheleau, T. A., Rashka, K., Staffeld, G., Stock, C., Sutton, D., and Merlo, D. J., Expression of Bacillus thuringiensis insecticidal crystal protein gene in tobacco plants. In “Molecular strategies for crop protection. UCLA symposia on molecular and cellular biology, New Series, vol. 46,“eds. Arntzen, C. J. and Ryan, C., Alan R. Liss, New York, pp. 345-353 (1987).
8. Murray, E. E., Rocheleau, T., Eberle, M., Stock, C., Sekar, V., and Adang, M. J., Analysis of unstable RNA transcripts of insecticidal crystal protein genes of Bacillus thuringiensis in transgenic plants and electroplated protoplasts. Plant Mol. Biol., 16, 1035-1050 (1991).
9. Van Aarsen, R., Soetaert, P., Siam, M., Dockh, J., Gossele, V., Seurinck, J., Reynaerts, A., and Cornelissen, M., cryIA(b) transcript formation in tobacco is inefficient. Plant Mol. Biol., 28, 513-524 (1995).
10. Diehn, S. H., Chiu, W. L., De Rocher, E. J., and Green, P. J., Premature polyadenylation at multiple sites within a Bacillus thuringiensis toxin gene-coding region. Plant Physiol., 117, 1433-1443 (1998).
11. Perlak, F. J., Fuchs, R. L., Dean, D. A., McPherson, S. L., and Fischhoff, D. A., Modification of the coding sequence enhances plant expression of insect control protein genes. Proc. Natl. Acad. Sci. USA, 88, 3324-3328 (1991).
12. Koziel, M. G., Beland, G. L., Bowman, C., Carozzi, N. B., Crenshaw, R., Crossland, L., Dawson, J., Desai, N., Hill, M., Kadwell, S., Launis, K., Lewis, K., Maddox, D., McPherson, K., Meghji, M. R., Merlin, E., Rhodes, R., Warren, G. W., Wright, M., and Evola, S. V., Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Biotechnology (N.Y.), 11, 194-200 (1993).
13. Adang, M. J., Brody, M. S., Cardineau, G., Eagan, N., Roush, R. T., Shewmaker, C. K., Jones, A., Oakes, J. V., and McBride, K. E., The reconstruction and expression of a Bacillus thuringiensis crylllA gene in protoplasts and potato plants. Plant Mol. Biol., 21, 1131-1145 (1993).
14. Jouanin, L., Bonnade-Bottino, M., Girard, C., Morrot, G., and Giband, M., Transgenic plants for insect resistance. Plant Science, 1-11 (1998).
15. Hills, D. S., “Agricultural insect pests of the tropics and their control,” Cambridge Univ. Press, Cambridge, U.K. (1983).
16. Yamamoto, T. and Powell, G. K., Bacillus thuringiensis crystal proteins: Recent advances in understanding its insecticidal activity. In “Advanced engineered pesticides,” ed. Kim, L., Marcel Dekker Inc., New York, pp. 3-42 (1993).
17. Poulsen, C., Fluhr, R., Kauffman, J. M., Boutry, M., and Chua, N. H., Characterization of an rbcS gene from Nicotiana plumbaginifolia and expression of an rbcS-CA Tchimeric gene in homologous heterologous nuclear background. Mol. Gen. Genet., 205, 193-200 (1986).
18. Shiota, N., Nakamura, K., Sakaki, T., Yabusaki, Y., and Ohkawa, H., Herbicide-resistant tobacco plants expressing the fused enzyme between rat cytochrome p4501Al (CYP1A1) and yeast NADPH-cytochrome p450 oxidoreductase. Plant Physiol., 106, 17-23 (1994).
19. Sambrook, J., Fritsch, F. F., and Maniatis, T., Molecular cloning: A laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, NY, ed. 2 (1989).
20. Christov, N., Shiota, N., and Ohkawa, H., Spodoptera litura tolerant transgenic tobacco plants expressing genetically modified crylAb genes from Bacillus thuringiensis. Biotechnology & Biotechnol. Eq., 8-16 (1998).
21. Horsh, R. B., Fry, J. E., Hoffman, N. L., Eichholtz, D., Rogers, S. G., and Fraley, R. T., A simple and general method for transferring genes into plants. Science, 227, 1229-1231 (1985).
22. Delaporta, S. L., Wood, J., and Hicks, J. B., Maize DNA miniprep. In “Molecular biology of plants: A laboratory course manual,” eds. Malmberg, R., Messing, J., and Sussex, I., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 36-37 (1985).
23. Stewart, C. N., Jr., Adang, M. J., All, J. N., Boerma, H. R., Cardineau, G., Tucker, D., and Parrott, W. A., Genetic transformation, recovery, and characterization of fertile soybean transgenic for a synthetic Bacillus thuringiensis crylAc gene. Plant Physiol., 112, 121-129 (1996).
24. Murray, E. E., Lotzer, J., and Eberle, M., Codon usage in plant genes. Nucleic Acids Res., 17, 477-498 (1989).
25. Dean, C., Tamaki, S., Dunsmuir, P., Favreau, M., Katayama, C., Dooner, H., and Bedbrook, J., mRNA of several plant genes are polyadenylated at multiple sites in vivo. Nucleic Acids Res., 14, 2229-2240 (1986).
26. Ohme-Takagi, M., Taylor, C. B., Newmwn, T. C., and Green, P. J., The effect of sequences with high AU content on mRNA stability in tobacco. Proc. Natl. Acad. Sci. USA, 90, 11811-11815 (1993).
27. Gniadkowski, M., Hemmings-Mieszczak, M., Klahre, U., Hong-Xiang, L., and Filipowicz, W., Characterization of intron-ic uridine-rich sequence elements acting as possible targets for nuclear proteins during pre-mRNA splicing in Nicotiana plum-baginifolia. Nucleic Acids Res., 24, 619-627 (1996).
28. Wong, E. Y., Hironaka, C. M., and Fischhoff, D. A. Arabidop-sis thaliana small subunit leader and transit peptide enhance the expression of Bacillus thuringiensis proteins in transgenic plants. Plant Mol. Biol., 20, 81-93 (1992).
29. Dalta, R. S. S., Bekkaoui, F., Hammerlindl, J. K., Pilate, G., Dunstan, D. I., and Crosby, W. L., Improved high-level constitutive foreign gene expression in plants using AMV RNA4 untranslated leader sequence. Plant Science, 94, 139-149 (1993).
30. Joshi, C. P., An inspection of the domain between putative TATA box and translation start site in 79 plant genes. Nucleic Acids Res., 15, 6643-6653 (1987).
31. Tang, J. D., Shelton, A. M., Van Rie, J., De Roeck, S., Moar, W. J., Roush, R. T., and Peferoen, M., Toxicity of Bacillus thuringiensis spore and crystal protein to resistant diamondback moth (Plutella xylostella). Appl. Environ. Microbiol., 62, 564-569 (1996).
32. Sutton, D. W., Havstad, P. K., and Kemp, J. D., Synthetic crylllA gene from Bacillus thuringiensis improved for high expression in plants. Transgenic Res., 1, 228-236 (1992).
33. Strizhov, N., Keller, M., Mathur, J., Koncz-Kalman, Z., Bosch, D., Prudovsky, E., Schell, J., Sneh, B., Koncz, C., and Zilberstein, A., A synthetic crylC gene, encoding a Bacillus thuringiensis deltα-endotoxin, confers Spodoptera resistance in alfalfa and tobacco. Proc. Natl. Acad. Sci. U.S.A., 93, 15012-15017 (1996).
34. Diehn, S. H., De Rocher, E. J., and Green, P. J., Problems that can limit the expression of foreign genes in plants: Lessons to be learned from B.t. toxin genes. In “Genetic engineering principles and methods,” ed. Setlow, J. K., vol. 18, Plenum Press, Upton, New York, pp. 83-99 (1996).
35. Van der Salm, T., Bosch, D., Honee, G., Feng, L., Munsterman, E., Bakker, P., Stiekema, W. J., and Visser, B., Insect resistance of transgenic plants that express modified Bacil-luss thuringiensis cryIA(b) and crylC genes: a resistance managementt strategy. Plant Mol. Biol., 2624, 51-59 (1994).
36. Mazier, M., Chaufaux, J., Sanchis, V., Lereclus, D., Giband, M., and Tourneur, J., The cryic gene from Bacillus thuringiensis provides protection against Spodoptera litoralis in young transgenic plants. Plant Science, 127, 179-190 (1997).
37. Kar, S., Basu, D., Das, S., Ramkrishnan, N. A., Mukherjee, P., Nayak, P., and Sen, S. K., Expression of cryIA(c) gene of Bacillus thuringiensis in transgenic chickpea plants inhibits development of pod-borer (Heliothis armigera) larvae. Transgenic Research, 6, 177-185 (1997).
38. Iannacone, R., Grieco, P. D., and Cellini, F., Specific sequence modifications of a cry3B endotoxin gene result in high levels of expression and insect resistance. Plant Mol. Biol., 34, 485-496. (1997).