Expression of cryIA(c) gene of Bacillus thuringiensis in transgenic chickpea plants inhibits development of pod-borer (Heliothis armigera) larvae

Transgenic Research

Volumn 6, Issue 2, 1997, Pages 177-185

  Kar, Sanchayita ^a,b   Basu, Debabrata ^a   Das, Sampa ^a   Ramkrishnan, Neeliyath A ^a   Mukherjee, Puspita ^a   Nayak, Pritilata ^a   Sen, Soumitra K ^a  

^a BOSE INSTITUTE   (India)

^b MISSISSIPPI STATE UNIVERSITY   (United States)

Author keywords

cryIA(c) gene; gene transfer; toxic to pod borers; transgenic chickpea

Indexed keywords

BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); CICER ARIETINUM; HELICOVERPA ARMIGERA; HELIOTHIS; HELIOTHIS ARMIGERA; INSECTA; NICOTIANA TABACUM; TOBACCO MOSAIC VIRUS;

EID: 0030932812     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018433922766     Document Type: Article

References (45)

1. Adang, M., BeBoer, D., Endres, J., Firozabady, E., Klein, J., Merlo, D., Murray, E., Rashka, K. and Stock, C. (1988) Biological pesticides and novel plant-pest resistance for Insect Pest Management. In Roberts, D.W. and Granados, R.R. (eds) Biotech. pp. 31-7. Boyce Thompson Institute for Plant Research, Cornell University.
2. Adang, M., Brody, M.S., Cardinean, G., Eagaw, N., Roush, R.T., Shewmaker, C.K., Jones, A., Oakes, J.V. and McBride, K.E. (1993) The reconstruction and expression of Bacillus thuringiensis cryIIIA gene in protoplasts of potato plants. Plant Mol. Biol. 21, 1131-45.
3. Barton, K.A., Whitelt, H.R. and Yang, K.S. (1987) Bacillus thuringiensis δ-endotoxin expressed in transgenic Nicotiana tabacum provides resistance to lepidopteran insects. Plant Physiol. 85, 1103-9.
4. Basu, D., Das, S., Bandhyopadhyaya, D. and Sen, S.K. (1991) Isolation and cloning of Bacillus thuringiensis var Kurstaki HD73 toxin gene and construction of a chimeric gene for expression in plants. Ind. J. Exptl Biol. 29, 1002-9.
5. Basu, D., Das, S., Nayak, P. and Sen, S.K. (1995) Monitoring functional property of the transgene through rapid amplification of cDNA ends in indica rice transformants. Curr. Sci. 68, 1140-4.
6. Bradford, M.M., (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye interaction. Anal. Biochem. 72, 248-54.
7. Cao, J., Duan, X., McElroy, D. and Wu, R. (1992) Regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. Plant Cell Rep. 11, 586-91.
8. Carozzi, N.B., Warren, G.W., Desai, N., Jayne, S.M., Lotstein, R., Rice, D.A., Evola, S. and Koziel, M.G. (1992) Expression of a chimeric CaMV35S Bacillus thuringiensis insecticidal protein gene in transgenic tobacco. Plant Mol. Biol. 20, 539-48.
9. Chomczynski, P. and Sacchi, N. (1987) Single step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-9.
10. Christou, P., McCabe, D., Martinell, B. and Wsain, W. (1990) Soybean genetic engineering commercial production of transgenic plants. Trends Biotechnol. 8, 145-51.
11. De Kathen and Jacobsen, H.J. (1990) Agrobacterium tumefaciens mediated transformation of Pisum sativum L. using binary and cointegrated vectors. Plant Cell Rep. 9, 276-9.
12. Dellaporta, S.L., Wood, J. and Hicks, J.B. (1983) A plant DNA minipreparation; version II. Plant Mol. Biol. Rep. 1, 19-21.
13. Eapen, S., Kofler, F., Gerdemann, M. and Schieder, O. (1987) Cultivar dependence of transformation rates in moth bean after cocultivation of protoplasts with Agrobacterium tumefaciens. Theor. Appl. Genet. 75, 207-10.
14. Fischhoff, D.A., Bowdish, K.S., Perlak, F.J., Marrone, P.G., McCormick, S.M., Niedermeyer, J.G., Dean, D.A., Kusano-Kretzmer, K., Mayor, E.J., Rochester, Rogers, S.G. and Fraley, R.T. (1987) Insect tolerant transgenic tomato plants. Bio/Technol. 5, 807-13.
15. Fontana, G.S., Santini, L., Caretto, S., Frugis, G. and Mariotti, D. (1993) Genetic transformation in the grain legume Cicer arietinum L. (chickpea). Plant Cell Rep. 12, 194-8.
16. Fujimoto, H., Itoh, K., Yamamoto, M., Kyozuka, J. and Shimamoto, K. (1993) Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis Bio/Technol. 11, 1151-5.
17. Gallie, Dr., Lucas, W.J. and Walbot, V. (1989) Visualing mRNA expression in plant protoplats: factors influencing efficient mRNA uptake and translation. Plant Cell 1, 301-11.
18. Gamborg, O.L., Miller, R.A. and Ojima, K. (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50, 151-8.
19. Hinchee, M.A.V., Connor-Ward, D.V., Newell, C.A., McDonnel, R.E., Sato, S.J., Gasser, C.S., Fischhoff, D.A., Re, D.B. Fraley, R.T. and Horsch, R.B. (1988) Production of transgenic soybean plants using Agrobacterium mediated transformation. Bio/Technol. 6, 915-22.
20. Hofte, H., de Greve, H., Seurrinck, J., Jansens, S., Mahillon, J., Ampe, C., Vandekerckhove, J., Vanderbruggen, H., Van Montagu, M., Zabeau, M. and Vaeck, M. (1986) Structural and functional analysis of a cloned δ-endotoxin of Bacillus thuringiensis Berliner 1715. Eur. J. Biochem. 161, 273-80.
21. Jobling, S.A. and Gehrke, L. (1987) Enhanced translation of chimeric messenger RNAs containing a plant viral untranslated leader sequence. Nature 325, 622-5.
22. Kozak, M. (1984) Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs. Nucl. Acid Res. 12, 857-72.
23. Koziel, M.G., Beland, G.L., Bowman, C., Carozzi, N.B., Crenshaw, R., Crossland, L., Dawson, J., Desai, N., Hill, M., Kadwell, S., Launis, K., Lewis, K., Maddox, D., McPherson, K., Meghji, M.R., Merlin, E., Rhodes, R., Warren, G.W., Wright, M. and Evola, S.V. (1993) Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Bio/Technol. 11, 194-200.
24. Laemmli, E.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-5.
25. Lyznik, L.A., Ryan, R.D., Ritchie, S.W. and Hodges, T.H. (1989) Stable co-transformation of maize protoplasts with gusA and neo genes. Plant Mol. Biol. 13, 151-61.
26. Mariotti, D., Fontana, G.S. and Santini, L. (1989) J. Genet. & Breed. 43, 77-82.
27. McBride, K.E., Svab, Z., Schaaf, D.J., Hogan, P.S., Stalker, D.M. and Maliga, P. (1995) Amplification of a chimeric Bacillus gene in chloroplasts leads to an extra ordinary level of an insecticidal protein in tobacco. Bio/Technol. 13, 362-5.
28. McCabe, D., Swain, W., Martinell, B. and Christou, P. (1988) Stable transformation of soybean (Glycine max) by particle acceleration. Bio/Technol. 6, 923-6.
29. Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15, 473-97.
30. Murray, E.E., Rochelean, T., Eberle, M., Stack, C., Sekar, V. and Adang, M. (1991) Analysis of unstable RNA transcripts of insecticidal crystal protein genes of Bacillus thuringiensis in transgenic plant and electroporated protoplasts. Plant Mol. Biol 16, 1035-50.
31. Olins, P.O., Devine, C.S., Rangwala, S.H. and Kavka, K.S. (1988) The T7 phage gene 10 leader RNA, a ribosome binding site that dramatically enhances the expression of foreign genes in Escherichia coli. Gene 73, 227-35.
32. Perlak, F.J., Deaton, R.U., Armstrong, T.A., Fuchs, R.L., Sims, S.R., Greenplate, J.T. and Fischhoff, D.A. (1990) Insect resistant cotton plants. Bio/Technol. 8, 939-43.
33. Perlak, F.J., Fuchs, R.L., Dean, D.A., McPherson, S.L. and Fischhoff, D.A. (1991) Modification of the coding sequence enhances plant expression of insect control protein genes. Proc. Natl. Acad. Sci., USA 88, 3324-8.
34. Perlak, F.J., Stone, T.B., Muskopf, Y.M., Peterson, L.J., Parker, G.B., McPherson, S.A., Wyman, J., Love, S., Reed, G., Biever, D. and Fischhoff, D.A. (1993) Genetically improved potatoes: protection from damage by Colorado potato beetles. Plant Mol. Biol. 22, 313-21.
35. Puonti-Kaerlas, J., Eriksson, T. and Engstrom, P. (1990) Production of transgenic pea (Pisum sativum L.) plants by Agrobacterium tumefaciens mediated gene transfer. Theor. Appl. Genet. 80, 246-52.
36. Puonti-Kaerlas, J., Eriksson, T. and Engstrom, P. (1992) Inheritance of a bacterial hygromycin phosphotransferase gene in the progeny of primary transgenic pea plants. Theor. Appl. Genet. 84, 443-50.
37. Rishi, A.S. (1993) Isolation and cloning of entomocidal crystal protein gene from Bacillus thuringiensis var berliner and its transfer to plant. Ph.D. Thesis, University of Calcutta, India.
38. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning 7.39. Cold Spring Harbour Laboratory, Cold Spring Harbour, NY.
39. Schnepf, H.E., Wong, H.C. and Whitely, H.R. (1985) The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence. J. Biol. Chem. 260, 6264-72.
40. Schroeder, H.E., Schotz, A.H., Wardley-Richardson, T., Spencer, D. and Higgins, T.J.V. (1993) Transformation and regeneration to two cultivars of Pea (Pisum sativum L.). Plant Physiol. 101, 751-7.
41. Sleat, D.E., Gallie, D.R., Jefferson, R.A., Bevan, M.W., Turner, P.C. and Wilson, T.M.A. (1987) Characterisation of the 5′ leader sequence of tobacco mosaic virus RNA as a general enhancer of translation in vitro. Gene 45, 217-25.
42. Stockhaus, J., Eckes, P., Blan, A., Schell, J. and Wilmitzer, L. (1987) Organ-specific and dosage-dependent expression of a leaf/stem specific gene from potato after tagging and transfer into potato and tobacco plants. Nucl. Acid Res. 15, 3479-91.
43. Vaeck, M., Reynaerts, A., Hofte, H., Jansens, S., DeBeuckeller, M., Dean, C., Zabeau, M., Van Montagu, M. and Leemans, J. (1987) Plants protected from insect attack. Nature 327, 33-7.
44. Walbot, G. and Gallie, D. (1991) Gene expression in rice. In Khush, G.S. and Toenniessen, G.H. (ed.) Rice Biotechnology, pp. 225-51. CAB International.
45. Wong, E.Y., Hironaka, C.M. and Fischhoff, D.A. (1992) Arabidopsis thaliana small subunit leader and transit peptide enhance the expression of Bacillus thuringiensis protein in transgenic plants. Plant Mol. Biol. 20, 81-93.