Autocatalytic processing of the streptococcal cysteine protease zymogen. Processing mechanism and characterization of the autoproteolytic cleavage sites

European Journal of Biochemistry

Volumn 263, Issue 1, 1999, Pages 145-151

  Doran, John D ^a,b   Nomizu, Motoyoshi ^a,c   Takebe, Sachiko ^a   Ménard, Robert ^a   Griffith, Douglas ^a   Ziomek, Edmund ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b VERTEX PHARMACEUTICALS   (United States)

^c HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Autoprocessing; Cysteine protease; Streptococcal pyrogenic exotoxin B (SpeB); Streptococcus

Indexed keywords

3 AMINOBENZOIC ACID; BACTERIAL ENZYME; CYSTEINE PROTEINASE; ENDOGENOUS PYROGEN; ENZYME PRECURSOR; EXOTOXIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; IN VITRO STUDY; MICHAELIS CONSTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROCESSING; REACTION TIME; STREPTOCOCCUS PYOGENES;

EID: 0033168439     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00473.x     Document Type: Article

References (28)

1. 1. Gerlach, D., Knoll, H., Kohler, W., Oregowski, J.H. & Hribalova, V. (1983) Isolation and characterisation of erythrogenic toxins. V. Communication: identity of erythrogenic toxin type B and streptococcal proteinase precursor. Zentralbl. Bakt. Hyg. I. Abt. Orig. A 255, 221-233.
2. 2. Hauser, A.R. & Schlievert, P.M. (1990) Nucleotide sequence of the streptococcal pyrogenic exotoxin type B gene and relationship between the toxin and the streptococcal proteinase precursor. J. Bacteriol. 172, 4536-4542.
3. 3. Kapur, V., Topouzis, S., Majesky, M.W., Li, L., Hamrick, M.R., Hamill, R.J., Patti, J.M. & Musser, J.M. (1993) A conserved Streptococcus pyogenes extracellular cysteine protease cleaves human fibronectin and degrades vitronectin. Micob. Pathog. 15, 327-346.
4. 4. Kapur, V., Majesky, M.W., Li, L., Black, R.A. & Musser, J.M. (1993) Cleavage of interleukin 1 beta (IL-1 beta) precursor to produce active IL-1 beta by a conserved extracellular cysteine protease from Streptococcus pyogenes. Proc. Natl Acad. Sci. U.S.A. 90, 7676-7680.
5. 5. Herwald, H., Collin, M. & Muller-Esterl, W. (1996) Streptococcal cysteine proteinase releases kinins: a virulence mechanism. J. Exp. Med. 184, 665-673.
6. 6. Berge, E. & Björk, L. (1995) Streptococcal cysteine proteinase releases biologically active fragments of streptococcal surface proteins. J. Biol. Chem. 270, 9862-9867.
7. 7. Lukomski, S., Sreevatsan, S., Amberg, C., Reichardt, W., Woischnik, M. & Podhielski, A. (1997) Inactivation of Streptococcus pyogenes extracellular cysteine protease significantly decreases mouse lethality of serotype M3 and M49 strains. J. Clin. Invest. 99, 2574-2580.
8. 8. Liu, T. & Elliott, S.D. (1965) Streptococcal proteinase: the zymogen to enzyme transformation. J. Biol. Chem. 240, 1138-1142.
9. 9. Liu, T., Neumann, S.D., Elliott, S.D., Moore, S. & Stein, W.H. (1963) Chemical properties of streptococcal proteinase and its zymogen. J. Biol. Chem. 238, 251-256.
10. 10. Barsumian, E.I., Cunningham, C.M., Schlievert, P.M. & Watson, D.W. (1978) Heterogeneity of group A streptococcal pyrogenic exotoxin type B. Infect. Immun. 20, 512-518.
11. 11. Reikofski, J. & Tao, B.Y. (1992) Polymerase chain reaction (PCR) techniques for site-directed mutagenesis. Biotech. Adv. 10, 535-547.
12. 12. Cull, M. & McHenry, C.S. (1990) Preparation of extracts from prokaryotes. Methods Enzymol. 182, 147.
13. 13. Szewczuk, A. & Prusak, E. (1985) Purification of γ-glutamyltransferase by immunoaffinity chromatography. In Affinity Chromatography - a Practical Approach (Dean, P.D.G., Johnson, W.S. & Middle, F.A., eds). pp. 119. IRL Press Limited, Oxford.
14. 14. Nomizu, M., Kuratomi, Y., Song, S.Y., Ponce, M.L., Hoffman, M.P., Powell, S.K., Miyoshi, K., Otaki, A., Kleinman, H.K. & Yamada, Y. (1997) Identification of cell binding sequences in mouse laminin gammal chain by systematic peptide screening. J. Biol. Chem. 272, 32198-32205.
15. 15. Elliott, S.D. & Liu, T.-Y. (1970) Streptococcal proteinase. Methods Enzymol. 19, 252-261.
16. 16. Barrett, A.J. & Kirschke, H. (1981) Cathepsin B, cathepsin H, and cathepsin L. Methods Enzymol. 80, 535-561.
17. 17. Volkov, A. & Jordan, F. (1996) Evidence for intramolecular processing of prosubtilisin sequestered on a solid support. J. Mol. Biol. 262, 595-599.
18. 18. Yonaha, K., Elliott, S.D. & Liu, T. (1982) Primary structure of zymogen of streptococcal proteinase. J. Prot. Chem. 1, 317-334.
19. 19. McQueney, M.S., Amegadzie, B.Y., D'Alessio, K., Hanning, C.R., McLaughlin, M.M., McNulty, D., Carr, S.A., Ijames, C., Kurdyla, J. & Jones, C.S. (1997) Autocatalytic activation of human cathepsin K. J. Biol. Chem. 272, 13955-13960.
20. 20. Ménard, R., Carmona, E., Takebe, S., Dufour, E., Plouffe, C., Mason, P. & Mort, J.S. (1998) Autocatalytic processing of recombinant human procathepsin L. Contribution of both intermolecular and unimolecular events in the processing of procathepsin L in vitro. J. Biol. Chem. 273, 4478-4484.
21. 21. Mach, L., Mort, J.S. & Glossl, J. (1994) Maturation of human procathepsin B. Proenzyme activation and proteolytic processing of the precursor to the mature proteinase, in vitro, are primarily unimolecular processes. J. Biol. Chem. 269, 13030-13035.
22. 22. Gerwin, B., Stein, W.H. & Moore, S. (1966) On the specificity of streptococcal proteinase. J. Biol. Chem 241, 3331-3339.
23. 23. Bhakdi, S., Roth, M., Sziegoleit, A. & Tranum-Jensen, J. (1984) Isolation and identification of two hemolytic forms of streptolysin-O. Infect. Immun. 46, 394-400.
24. 24. Pinkney, M., Kapur, V., Smith, J., Weller, U., Palmer, M., Glanville, M., Messner, M., Musser, J.M., Bhakdi, S. & Kehoe, M.A. (1995) Different forms of streptolysin O produced by Streptococcus pyogenes and by Escherichia coli expressing recombinant toxin: cleavage by streptococcal cysteine protease. Infect. Immun. 63, 2776-2779.
25. 25. Kortt, A.A. & Liu, T.Y. (1973) On the mechanism of action of streptococcal proteinase. II. Comparison of the kinetics of proteinase-and papain-catalysed hydrolysis of N-acylamino acid esters. Biochemistry 12, 320-327.
26. 26. Bustin, M., Lin, M.C., Stein, W.H. & Moore, S. (1970) Activity of the reduced zymogen of streptococcal proteinase. J. Biol. Chem. 245, 846-849.
27. 27. Gubba, S., Low, D.E. & Musser, J.M. (1998) Expression and characterisation of group A Streptococcus extracellular cysteine protease recombinant mutant proteins and documentation of sero-conversion during human invasive disease episodes. Infect. Immun. 66, 765-770.
28. 28. Vernet, T., Khouri, H.E., Laflamme, P., Tessier, D.C., Musil, R., Gour-Salon, B.J., Storer, A.C. & Thomas, D.Y. (1991) Processing of the papain precursor. Purification of the zymogen and characterisation of its mechanism of processing. J. Biol. Chem. 266, 21451-21457.