Use of the green fluorescent protein variant (YFP) to monitor MetArg human proinsulin production in Escherichia coli

Protein Expression and Purification

Volumn 16, Issue 2, 1999, Pages 315-323

  Daabrowski, Slawomir ^a   Brillowska, Anna ^a   Kur, Józef ^a  

^a GDANSK UNIVERSITY OF TECHNOLOGY   (Poland)

Author keywords

Expression plasmid; Insulin; Recombinant protein; Reporter gene

Indexed keywords

CARBOXYPEPTIDASE B; FLUORESCENCE ANALYSIS; GREEN FLUORESCENT PROTEIN; ISOPROPYL BETA THIOGALACTOSIDE; MONITORING; PLASMID; PROINSULIN; PROTEIN EXPRESSION; TRYPSIN;

ESCHERICHIA COLI;

EID: 0033166482     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1999.1072     Document Type: Article

References (25)

1. Chalfie M., Tu Y., Euskirchen G., Ward W. W., Prasher D. C. Green fluorescent protein as a marker for gene expression. Science. 263:1994;802-805.
2. Prasher D. C., Eckenrode V. K., Ward W. W., Prendergast F. G., Cormier M. J. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 111:1992;229-233.
3. Inouye S., Tsuji F. I. Aequorea green fluorescent protein. Expression of the gene and fluorescence characteristics of the recombinant protein. FEBS Lett. 341:1994;277-280.
4. Wang S., Hazelrigg T. Implications for bcd mRNA localization from spatial distribution of exu protein in Drosophila oogenesis. Nature. 369:1994;400-403.
5. Inouye S., Tsuji F. I. Evidence for redox forms of the Aequorea green fluorescent protein. FEBS Lett. 351:1994;211-214.
6. Heim R., Prasher D. C., Tsien R. Y. Wavelength mutations and posttranslational autooxidation of green fluorescent protein. Proc. Natl. Acad. Sci. USA. 91:1994;12501-12504.
7. Yang T. T., Cheng L., Kain S. R. Optimized codon usage and chromophore mutations provide enhanced sensitivity with the green fluorescent protein. Nucleic Acids Res. 24:1996;4592-4593.
8. Cormack B. P., Valdivia R. H., Falkow S. FACS-optimized mutants of the green fluorescent protein (GFP). Gene. 173:1996;33-38.
9. CLONTECHniques. XII:April 1997;16-17.
10. Wachter R. M., Elsliger M. A., Kallio K., Hanson G. T., Remington S. J. Structural basis of spectral shifts in the yellow-emission variants of green fluorescent protein. Structure. 15:1998;1267-1277.
11. Chance R. E., Hoffmann J. A., Kroell E. P., Johnson M. G., Schirmer E. W., Bromer W. W. Rich D., And Gross E. Peptides: Proceedings of the Seventh American Peptide Chemistry Symposium. 1981;721-728 Pierce Chemical Co. Rockford.
12. Frank B. H., Pettee J. M., Zimmerman R. E., Burck P. J. Rich D., Gross E. Peptides: Proceedings of the Seventh American Peptide Chemistry Symposium. 1981;729-739 Pierce Chemical Co. Rockford.
13. Chan S. J., Weiss J., Konrad M., White T., Bahl C., Yu S. D., Marks D., Steiner D. F. Biosynthesis and periplasmic segregation of human proinsulin in Escherichia coli. Proc. Natl. Acad. Sci. USA. 78:1981;5401-5404.
14. Thim L., Hansen M. T., Norris K., Hoegh I., Boel E., Forstrom J., Ammerer G., Fill N. P. Secretion and processing of insulin precursors in yeast. Proc. Natl. Acad. Sci. USA. 83:1986;6766-6770.
15. Goeddel D. V., Kleid D. G., Bolivar F., Heyneker H. L., Yansura D. G., Crea R., Hirose T., Kraszewski A., Itakura K., Riggs A. D. Expression in Escherichia coli of chemically synthesized genes for human insulin. Proc. Natl. Acad. Sci. USA. 76:1979;106-110.
16. Talmadge K., Kaufman J., Gilbert W. Bacteria mature preproinsulin to proinsulin. Proc. Natl. Acad. Sci. USA. 77:1980;3988-3992.
17. Sung W. L., Yao F. L., Zahab D. M., Narang S. A. Short synthetic oligodeoxyribonucleotide leader sequences enhance accumulation of human proinsulin synthesized in Escherichia coli. Proc. Natl. Acad. Sci. USA. 83:1986;561-565.
18. Neumann U., Khalaf H., Rimpler M. Quantitation of electrophoretically separated proteins in the submicrogram range by dye elution. Electrophoresis. 15:1994;916-921.
19. Chen J. Q., Zhang H. T., Hu M. H., Tang J. G. Production of human insulin in an E. coli system with Met-Lys-human proinsulin as the expressed precursor. Appl. Biochem. Biotechnol. 55:1995;5-15.
20. Jonasson P., Nilsson J., Samuelsson E., Moks T., Stahl S., Uhlen M. Single-step trypsin cleavage of a fusion protein to obtain human insulin and its C peptide. Eur. J. Biochem. 236:1996;656-661.
21. Belagaje R. M., Reams S. G., Ly S. C., Prouty W. F. Increased production of low molecular weight recombinant proteins in Escherichia coli. Protein Sci. 6:1997;1953-1962.
22. Albano C. R., Randers-Eichhorn L., Bentley W. E., Rao G. Green fluorescent protein as a real time quantitative reporter of heterologous protein production. Biotechnol. Prog. 14:1998;351-354.
23. Aran J. M., Gottesman M. M., Pastan I. Construction and characterization of bicistronic retroviral vectors encoding the multidrug transporter and beta-galactosidase or green fluorescent protein. Cancer Gene Ther. 5:1998;195-206.
24. Mosser D. D., Caron A. W., Bourget L., Jolicoeur P., Massie B. Use of a dicistronic expression cassette encoding the green fluorescent protein for the screening and selection of cells expressing inducible gene products. Biotechniques. 156:1997;158-161.
25. Metz M. Z., Pichler A., Kuchler K., Kane S. E. Construction and characterization of single-transcript tricistronic retroviral vectors using two internal ribosome entry sites. Somat. Cell Mol. Genet. 24:1998;53-69.