Site-specific mutagenesis of bacteriophage T7 RNA polymerase: Effect of met-635 and ser-633 substitutions on the enzyme properties

Molekulyarnaya Biologiya

Volumn 33, Issue 4, 1999, Pages 598-602

  Rusakova, E E ^a   Yankin, A P ^a   Memelova, L V ^a   Tunitskaya, V L ^a   Kochetkov, S N ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

DNA DIRECTED RNA POLYMERASE; METHIONINE; SERINE;

ARTICLE; BACTERIOPHAGE T7; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; PROMOTER REGION; SITE DIRECTED MUTAGENESIS;

BACTERIOPHAGE T7; DNA-DIRECTED RNA POLYMERASES; KINETICS; METHIONINE; MUTAGENESIS, SITE-DIRECTED; PROMOTER REGIONS (GENETICS); SERINE;

EID: 0033155249     PISSN: 00268984     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Delarue M., Poch O., Tarda N., Moms D., Argos P. H Protein Eng. 1990. V. 3. P. 461-467.
2. Kostyuk DA., Dragan S.M., Lyakhov D.L., Rechinsky V.O., Tunitskaya V.L., Chernov B.K., Kochetkov S.N. // FEBS Lett. 1995. V. 369. P. 165-168.
3. Maksimova T.G., Mustaev A.A., Zaychikov E.F., Lyakhov D.L., AkbarovA.K., Luchin S.V.,Rechinsky V.O., Chernov B.K., Kochetkav S.N. // Ew. J. Biochem. 1991. V. 195. P. 841-847.
4. Sproal B.S., Gait MJ. // Qligonucleotide synthesis: a practical approach. Oxford: IRL Press, 1984. P. 83-115.
5. Promega protocol and applications guide. Promega Corporation Inc., 1996.
6. Tunitskaya V.L., Akbarov A,Kh., Luchin S.V., MemelovaL.V., Rechimky V.O., Kochetkov S.N. // Eur. J. Biochem. 1990. V. 191. P. 99-103.
7. Sausa R., Chung Y.T., Rose J.P., Wang B.-C. // Nature. 1993. V. 364. P. 593-599.
8. Double S., Tabor S., Long AM., Richardson C.C., Ellenberg T. // Nature. 1998. V. 391. P. 251-258.
9. Tabor S., Richardson C.C. // Proc. Natl. Acad. Sci. USA. 1995. V. 92. P. 6339-6343.
10. Rusakova E.E., Tunitskaya V.L., Memelova L.V., Kochetkova S.V., Kostyuk D A., Kochetkov S.N. // FEBS Lett. 1998. V. 423. P. 189-192.
11. Huang Y Eckstein F., PadillaR., SousaR. // Biochemistry. 1997. V. 36. P. 8231-8242.
12. Rechinsky V.O., Kostyuk DA., Tunitskaya V.L., Kochetkov S.N. // FEBS Lett. 1992. V. 306. P. 129-132.