Stable expression of protective protein/cathepsin A-green fluorescent protein fusion genes in a fibroblastic cell line from a galactosialidosis patient. Model system for revealing the intracellular transport of normal and mutated lysosomal enzymes

Biochemical Journal

Volumn 340, Issue 2, 1999, Pages 467-474

  Naganawa, Yasunori ^a,b   Itoh, Kohji ^a   Shimmoto, Michie ^a   Kamei, Sachiko ^b   Takiguchi, Kyoko ^c   Doi, Hirofumi ^c   Sakuraba, Hitoshi ^a  

^a TOKYO METROPOLITAN INSTITUTE OF MEDICAL SCIENCE   (Japan)

^b TOKYO MEDICAL AND DENTAL UNIVERSITY   (Japan)

^c FUJITSU LABORATORIES LTD   (Japan)

Author keywords

Bioimaging; Lysosomal disease; Mutant gene product

Indexed keywords

ADENOSINE TRIPHOSPHATASE; BAFILOMYCIN; CARBOXYPEPTIDASE C; CHIMERIC PROTEIN; COMPLEMENTARY DNA; ENZYME PRECURSOR; GREEN FLUORESCENT PROTEIN; LEUPEPTIN; LYSOSOME ENZYME;

ARTICLE; CHIMERA; CONFOCAL LASER MICROSCOPY; CYTOPLASM; ENDOPLASMIC RETICULUM; ENZYME ACTIVITY; FIBROBLAST CULTURE; FLUORESCENCE; FUSION GENE; GALACTOSIALIDOSIS; GENE EXPRESSION; GOLGI COMPLEX; HUMAN; HUMAN CELL; IMMUNOREACTIVITY; INTRACELLULAR TRANSPORT; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BASE SEQUENCE; BIOLOGICAL TRANSPORT; CARBOXYPEPTIDASE C; CARBOXYPEPTIDASES; CELL LINE; DNA, COMPLEMENTARY; FIBROBLASTS; GREEN FLUORESCENT PROTEINS; HUMANS; LEUPEPTINS; LUMINESCENT PROTEINS; LYSOSOMAL STORAGE DISEASES; LYSOSOMES; MACROLIDES; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECOMBINANT FUSION PROTEINS;

EID: 0033151872     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3400467     Document Type: Article

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