Mixed reconstitution of mutated subunits of HIV-1 reverse transcriptase coexpressed in Escherichia coli - Two tags tie it up

European Journal of Biochemistry

Volumn 261, Issue 1, 1999, Pages 10-18

  Maier, Gottfried ^a   Dietrich, Ursula ^b   Panhans, Barbara ^b   Schröder, Britta ^b   Rübsamen Waigmann, Helga ^b   Cellai, Luciano ^c   Hermann, Thomas ^a   Heumann, Hermann ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b INSTITUTE FOR TUMOR BIOLOGY AND EXPERIMENTAL THERAPY   (Germany)

^c CNR   (Italy)

Author keywords

Coexpression; GST fusion; Helix clamp motif; Heterologous expression; HIV 1 reverse transcriptase

Indexed keywords

AGAROSE; DOUBLE STRANDED DNA; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; NITRILOTRIACETIC ACID; RNA DIRECTED DNA POLYMERASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; DIMERIZATION; DNA BINDING; ELECTROPHORETIC MOBILITY; ENZYME BINDING; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; GENETIC MANIPULATION; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION;

BASE SEQUENCE; BINDING SITES; CLONING, MOLECULAR; DIMERIZATION; DNA, VIRAL; ESCHERICHIA COLI; GENE EXPRESSION; HISTIDINE; HIV-1; HIV-1 REVERSE TRANSCRIPTASE; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN CONFORMATION; RECOMBINANT FUSION PROTEINS;

EID: 0033118205     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00304.x     Document Type: Article

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