Removal of chromosomal DNA by Mg2+ in the lysis buffer: An improved lysis protocol for preparing Escherichia coli whole-cell lysates for sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Analytical Biochemistry

Volumn 246, Issue 2, 1997, Pages 263-264

  Chen, Min ^a   Christen, Philipp ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ELECTROPHORESIS; ESCHERICHIA COLI; SULFUR COMPOUNDS;

CHROMOSOMAL DNAS; LYSIS BUFFER; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; WHOLE CELL LYSATES;

SODIUM DODECYL SULFATE;

DNA; MAGNESIUM ION;

ARTICLE; CHROMOSOME; DNA CLEAVAGE; DNA DETERMINATION; ESCHERICHIA COLI; GENE TARGETING; IMMUNOBLOTTING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SILVER STAINING;

ESCHERICHIA COLI;

EID: 0031569330     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.1997.2021     Document Type: Article

References (3)

1. J. Sambrook, E. F. Fritsch, T. Maniatis, 1989, Molecular Cloning. A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
2. F. C. Neidhardt, J. L. Ingraham, M. Schaechter, 1990, Physiology of the Bacterial Cell. A Molecular Approach, Sinauer, Sunderland, MA.
3. Laemmli U. K. Nature. 227:1970;680-685.