A novel system for efficient gene expression and monitoring of bacteria in aquatic environments

Environmental Microbiology

Volumn 1, Issue 2, 1999, Pages 175-182

  Espinosa Urgel, Manuel ^a   Kolter, Roberto ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PHOTOPROTEIN;

ARTICLE; CULTURE MEDIUM; ENZYMOLOGY; ESCHERICHIA COLI; GENETIC TRANSCRIPTION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLASMID; PROMOTER REGION;

BASE SEQUENCE; CULTURE MEDIA; ESCHERICHIA COLI; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; GREEN FLUORESCENT PROTEINS; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; PLASMIDS; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS; TRANSCRIPTION, GENETIC; WATER MICROBIOLOGY;

EID: 0033108063     PISSN: 14622912     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-2920.1999.00025.x     Document Type: Article

References (19)

1. Amann, R.I., Krumholz, L., and Stahl, D.A. (1990) Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic and environmental studies in microbiology. J Bacteriol 172: 762-770.
2. Andersen, J.B., Sternberg, C., Poulsen, L.K., Bjørn, S.P., Givskov, M., and Molin, S. (1998) New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 64: 2240-2246.
3. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Smith, J.A., Seidman, J.G., et al. (1987) Current Protocols in Molecular Biology. New York: John Wiley & Sons.
4. Bloemberg, G.V., O'Toole, G.A., Lugtenberg, B.J.J., and Kolter, R. (1997) Green fluorescent protein as a marker for Pseudomonas spp. Appl Environ Microbiol 63: 4543-4551.
5. Chen, F., González, J.M., Dustman, W.A., Moran, M.A., and Hodson, R.E. (1997) In situ reverse transcription, an approach to characterize genetic diversity and activities of prokaryotes. Appl Environ Microbiol 63: 4907-4913.
6. Connell, N., Han, Z., Moreno, F., and Kolter, R. (1987) An E. coli promoter induced by the cessation of growth. Mol Microbiol 1: 195-201.
7. Espinosa-Urgel, M., and Kolter, R. (1998) Escherichia coli genes expressed preferentially in an aquatic environment. Mol Microbiol 28: 325-332.
8. Hidalgo, E., Limon, A., and Aguilar, J. (1996) A second Escherichia coli gene with similarity to gapA. Microbiología 12: 99-106.
9. Kohara, Y., Akiyama, K., and Isono, K. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50: 495-508.
10. Leff, L.G., and Leff, A.A. (1996) Use of green fluorescent protein to monitor survival of genetically engineered bacteria in aquatic environments. Appl Environ Microbiol 62: 3486-3488.
11. de Lorenzo, V. (1994) Designing microbial systems for gene expression in the field. Trends Biotechnol 12: 365-371.
12. Maniatis, T., Fritsch, E.F., and Sambrook, J. (1982) Molecular Cloning A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
13. Matin, A., Little, C.D., Fraley, C.D., and Keyhan, M. (1995) Use of starvation promoters to limit growth and selectively enrich expression of trichloroethylene- and phenol-transforming activity in recombinant Escherichia coli. Appl Environ Microbiol 61: 3323-3328.
14. Miller, J.H. (1972) Experiments in Molecular Genetics. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
15. Newman, D.K., Beveridge, T.J., and Morel, F.M.M. (1997) Precipitation of arsenic trisulfide by Desulfotomaculum auripigmentum. Appl Environ Microbiol 63: 2022-2028.
16. Simons, R.W., Houman, F., and Kleckner, N. (1987) Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene 53: 85-96.
17. Tolker-Nielsen, T., Holmstrøm, K., and Molin, S. (1997) Visualization of specific gene expression in individual Salmonella typhimurium cells by in situ PCR. Appl Environ Microbiol 63: 4196-4203.
18. Webb, C.D., Decatur, A., Teleman, A., and Losick, R. (1995) Use of green fluorescent protein for visualization of cell-specific gene expression and subcellular protein localization during sporulation in Bacillus subtilis. J Bacteriol 177: 5906-5911.
19. Wilson, M., and Lindow, S.E. (1993) Release of recombinant microorganisms. Annu Rev Microbiol 47: 913-944.