Detection of DNA damages and repair in human culture cells with simulated space radiation

Acta Astronautica

Volumn 44, Issue 7, 1999, Pages 561-567

  Nagaoka, S ^a   Nakano, T ^a   Endo, S ^b   Onizuka, T ^b   Kagawa, Y ^b   Fujitaka, K ^c   Ohnishi, K ^d   Takahashi, A ^d   Ohnishi, T ^d  

^a JAPAN AEROSPACE EXPLORATION AGENCY   (Japan)

^b TORAY RESEARCH CENTER INC   (Japan)

^c NATIONAL INSTITUTE OF RADIOLOGICAL SCIENCES   (Japan)

^d NARA MEDICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ASSAYS; CELLS; DNA; ELECTROPHORESIS; ION BEAMS; IRRADIATION; PARTICLE ACCELERATORS;

CARBON ION BEAMS; COMET ASSAY; DNA DAMAGE; DNA REPAIR; POLYMERASE CHAIN REACTION; SPACE RADIATION;

RADIATION DAMAGE;

CARBON; DNA; HEAVY ION; RNA;

ARTICLE; CELL CULTURE; COMET ASSAY; DNA DAMAGE; DNA REPAIR; FIBROBLAST; GENE EXPRESSION REGULATION; HUMAN; MAGNETIC AND ELECTROMAGNETIC EQUIPMENT; POLYMERASE CHAIN REACTION; RADIATION EXPOSURE; RADIATION RESPONSE; TIME; TUMOR SUPPRESSOR GENE;

CARBON; CELLS, CULTURED; COMET ASSAY; DNA; DNA DAMAGE; DNA REPAIR; DOSE-RESPONSE RELATIONSHIP, RADIATION; FIBROBLASTS; GENE EXPRESSION REGULATION; GENES, P53; HEAVY IONS; HUMANS; PARTICLE ACCELERATORS; POLYMERASE CHAIN REACTION; RNA; TIME FACTORS;

EID: 0033107676     PISSN: 00945765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0094-5765(99)00067-3     Document Type: Article

References (26)

1. J. E. Ward, Molecular mechanisms of radiation- induced damage to nucleic acid, in: J. T. Left and H. I. Adler(Eds.), Advances in Radiation Biology, Vol. 5, Academic Press, New York., pp. 182-239 (1975).
2. O. Ostling, and K. J. Johanson, Microelectrophoretic study of radiation- induced DNA damages in individual mammalian cells. Biochem. Biophys. Res. Commun. 26, 291-298 (1984).
3. N. P. Singh, M. T. McCoy, R. R. Tice, and E. L. Schneider, A simple technique for quantitation of low leaves of DNA damage in individual cells. Exp. Cell Res. 175, 184-191 (1998).
4. P. L. Olive, J. P. Banath, and R. E. Durand, Detection of etoposide resistance b measuring DNA damage in individual chinese hamster cells. J. Natl. Cancer Institute 82, 779-783 (1990).
5. C. M. Gedik, S. W. B. Ewen, and A. R. Collins, Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int. J. Radiat. Biol. 62, 313-320 (1992).
6. D. W. Fairbirn, P. L. Olive, and K. L. O'Neill, The comet assay: A comprehensive review. Mutat. Res. 339, 37-59 (1995).
7. M. Y. Arcellana - Panlilio, and G. A. Schultz, Analysis of messenger RNA. Methods Enzymol. 225, 303-328 (1993).
8. H. Akiyama, S. Kanai, M. Hirano, Y. Kumei, H. Shimokawa, H. Katano, E. Hara, S. Song, C. Mukai, and S. Nagaoka, An improved quantitative RT- PCR fluorescent method for analysis of gene transcripts in the STS-65 space shuttle experiment. J. Biotech. 47, 325-333 (1996).
9. W. Lu, O Han, J. Yuan, and A. Jean-Marie, Mukkti-target PCR analysisi by capillary electrophoresis and laser-induced flourescence. Nature 368, 296-271 (1994).
10. E. Kamiskas, and J. C. Li, DNA fragmentation in permeabilized cells and nuclei. Biochem. J. 261, 17-21 (1989).
11. N. P. Singh, R. R. Tice, R. E. Stephens, and E. L. Schneider, A microgel electrophoresis technique for the direct quantitation of DNA damage and repair in individual fibroblasts cultured on microscope slides. Mutat. Res. 252,289-296 (1991).
12. T. R. Tritton, and G. Lee, The anticancer agent adriamycin can be actively cytotoxic without entering cells. Science 217, 248-250 (1982).
13. M. Frankenberg- Schwager, Induction, repair and biological relevance of radiation-induced DNA lesions in eukaryotic cells. Radiat. Environ., Biophys. 29, 272-292 (1990).
14. J. T. Lett Damage to cellular DNA from particulate radiation, the efficacy of its processing and the radio-sensitivity of mammalian cells. Emphasis on DNA double-strand breaks and chromatin breaks. Radia. Environ. Biophys. 31, 257-277 (1992).
15. Vijayalaxmi, R. R. Tice, and G. H. S. Strauss, Assessment of radiation-induced DNA damage in human blood lymphocytes using the signal-cell gel electrophoresis. Exp. Cell Res. 198, 259-267 (1992).
16. P.L. Olive, D. Wlodek, R.E. Durand, and J. P. Banath, Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell. Res. 198, 259-267 (1992).
17. P. R. Cook, and I. A. Brazell, Spectrofluorometric measurement of the binding of ethidium to super helical DNA from cell nuclei. Eur. J. Biochem. 84,465-477 (1978).
18. D. T. Godhead, Models to link DNA damage to ryes for final cellular effects. In: The Early Effects of Radiation on DNA. Edited by E. M. Fielder and P. Only (Springer-Verlag, Berlin), pp. 271-286 (1991).
19. D. T. Godhead, R. J. Munson, J. Tracker, and R. Coy, Mutation and inactivation of cultured mammalian cells exposed to beams of accelerated heavy ions. IV. Biophysical interpretation. Int. J. Radiat. Biol. 37, 135-167 (1980).
20. J. F. Ward, DNA damage produced by ionizing radiation in mammalian cells: identities, mechanisms of formation, and reparability. Progress in Nucleic Acids Research and Molecular Biology 35, 95-125 (1988).
21. M. N. Cornforth, and J. S. Bedford, X-ray-induced breakage and rejoining of human interface chromosomes. Science 222, 1141-1143 (1983).
22. M. Lobrich, B. Rydberg, and P. K. Cooper, Repair of x-ray-induced DNA double-strand breaks in specific not I restriction fragments in human fibroblasts: Joining if correct and incorrect ends. Proc. Natl. Acad. Sci. USA 92, 12050-12054 (1995).
23. V. A. Zakian, ATM-related genes: What do they tell us about functions of the human gene? Cell 82, 685-687 (1995).
24. S. J. Elledge, Cell cycle checkpoints: Preventing an identity crisis. Science 274, 1664-1627 (1996).
25. P. A. Jeggo, G. E. Taccioli, and S. P. Jackson, Menage a trois: Double strand break repair, V(D) J recombination and DNA-PK. BioEssays 17, 949-957 (1995).
26. D. T. Weaver, What to do at an end: DNA double-strand-break repair. TIG 11, 388-392 (1995).