High-affinity calcium indicators underestimate increases in intracellular calcium concentrations associated with excitotoxic glutamate stimulations

Neuroscience

Volumn 89, Issue 1, 1999, Pages 91-100

  Stout, A K ^a   Reynolds, I J ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

Author keywords

Calcium Green 5N; Excitotoxicity; Fluo 3; Fura 2; Intracellular calcium; Mag fura 2

Indexed keywords

CALCIUM; CALCIUM GREEN 5N; EXCITOTOXIN; FLUO 3; FURA 2; FURAPTRA; GLUTAMIC ACID; GLYCINE; INDICATOR; KAINIC ACID; MAGNESIUM; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BINDING AFFINITY; BRAIN NERVE CELL; CALCIUM CELL LEVEL; CONTROLLED STUDY; DEPOLARIZATION; FETUS; FOREBRAIN; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; RAT;

ANILINE COMPOUNDS; ANIMALS; BIOLOGICAL TRANSPORT; CALCIUM; EXCITATORY AMINO ACID AGONISTS; FLUORESCENT DYES; FURA-2; GLUTAMIC ACID; GLYCINE; KAINIC ACID; MAGNESIUM; NEURONS; NEUROTOXINS; ORGANIC CHEMICALS; POTASSIUM CHLORIDE; RATS; RATS, SPRAGUE-DAWLEY; XANTHENES;

EID: 0033105949     PISSN: 03064522     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0306-4522(98)00441-2     Document Type: Article

References (31)

1. Almers W. and Neher E. (1985) The Ca signal from fura-2 loaded mast cells depends strongly on the method of dye-loading. Fedn Eur. Biochem. Socs Lett. 192, 13-18.
2. Bozyczko-Coyne D., McKenna B. W., Connors T. J. and Neff N. T. (1993) A rapid fluorometric assay to measure neuronal survival in vitro. J. Neurosci. Meth. 50, 205-216.
3. 2+ in cultured cortical neurons. Neuron 11, 751-757.
4. Choi D. W. (1987) Ionic dependence of glutamate neurotoxicity. J. Neurosci. 7, 369-379.
5. Choi D. W., Maulucci-Gedde M. and Kriegstein A. R. (1987) Glutamate neurotoxicity in cortical cell culture. J. Neurosci. 7, 357-368.
6. Dubinsky J. M. and Rothman S. M. (1991) Intracellular calcium concentrations during "chemical hypoxia" and excitotoxic neuronal injury. J. Neurosci. 11, 2545-2551.
7. Eimerl S. and Schramm M. (1994) The quantity of calcium that appears to induce neuronal death. J. Neurochem. 62, 1223-1226.
8. 2+ indicators with greatly improved fluorescence properties. J. Biol. Chem. 6, 3440-3450.
9. 2+ accumulation in cortical cell culture correlates with subsequent neuronal degeneration. J. Neurosci. 13, 1993-2000.
10. Hartley Z. and Dubinsky J. M. (1993) Changes in intracellular pH associated with glutamate excitotoxicity. J. Neurosci. 13, 4690-4699.
11. 2+ and other metals. In Handbook of Fluorescent Probes and Research Chemicals (ed. Spence M. T. Z.), 6th edn, pp. 503-550. Molecular Probes, Inc., Eugene, Oregon.
12. 2+ using mag-fura-2. Am. J. Physiol. 32, C300-C307.
13. Hyrc K., Handran S. D., Rothman S. M. and Goldberg M. P. (1996) Ionized intracellular calcium concentration predicts excitotoxic neuronal death: Observations with low-affinity fluorescent calcium indicators. J. Neurosci. 17, 6669-6677.
14. Kiedrowski L. and Costa E. (1995) Glutamate-induced destabilization of intracellular calcium concentration homeostasis in cultured cerebellar granule cells: Role of mitochondria in calcium buffering. Molec. Pharmac. 47, 140-147.
15. Konishi M., Olson A., Hollingworth S. and Baylor S. M. (1988) Myoplasmic binding of fura-2 investigated by steady-state fluorescence and absorbance measurements. Biophys. J. 54, 1089-1104.
16. Konishi M., Hollingworth S., Harkins A. B. and Baylor S. M. (1991) Myoplasmic calcium transients in intact frog skeletal muscle fibers monitored with the fluorescent indicator furaptra. J. Gen. Physiol. 97, 271-301.
17. Lattanzio F. A. and Bartschat D. K. (1991) The effect of ph on rate constants, ion selectivity and thermodynamic properties of fluorescent calcium and magnesium indicators. Biochem. Biophys. Res. Commun. 177, 184-191.
18. 2+ influx underlies potent induction of injury. J. Neurosci. 15, 5457-5465.
19. Michaels R. L. and Rothman S. M. (1990) Glutamate neurotoxicity in vitro: Antagonist pharmacology and intracellular calcium concentrations. J. Neurosci. 10, 283-292.
20. Neher E. and Zucker R. S. (1993) Multiple calcium-dependent processes related to secretion in bovine chromaffin cells. Neuron 10, 21-30.
21. 2+ indicator furaptra during whole-cell patch-clamp recording. Pflügers Arch. 429, 587-591.
22. 2+ concentrations associated with glutamate excitotoxicity in cultured rat brain neurons. Neurosci. Lett. 162, 149-152.
23. Rajdev S. and Reynolds I. J. (1994) Glutamate-induced intracellular calcium changes and neurotoxicity in cortical neurons in vitro: Effect of chemical ischemia. Neuroscience 3, 667-679.
24. Raju B., Murphy E., Levy L. A., Hall R. D. and London R. E. (1989) A fluorescent indicator for measuring cytosolic free magnesium. Am. J. Physiol. 256, C540-C548.
25. Regehr W. G. and Atluri P. P. (1995) Calcium transients in cerebellar granule cell presynaptic terminals. Biophys. J. 68, 2156-2170.
26. Regehr W. G. and Tank D. W. (1992) Calcium concentration dynamics produced by synaptic activation of CA1 hippocampal pyramidal cells. J. Neurosci. 12, 4202-4223.
27. 2+ efflux in rat forebrain neurones in culture. J. Physiol., Lond. 492, 641-657.
28. Tymianski M., Charlton M. P., Carlen P. L. and Tator C. H. (1993) Source specificity of early calcium neurotoxicity in cultured embryonic spinal neurons. J. Neurosci. 13, 2085-2104.
29. 2+ loads by mitochondria in cultured rat hippocampal neurons. J. Neurophysiol. 76, 1611-1621.
30. 2+ exchange buffer glutamate-induced calcium loads in cultured cortical neurons. J. Neurosci. 15, 1318-1328.
31. 2+ indicators in frog skeletal muscle fibers. Biophys. J. 70, 896-916.