Kinetic characterization of Aspergillus niger N400 endopolygalacturonases I, II and C

European Journal of Biochemistry

Volumn 259, Issue 3, 1999, Pages 577-585

  Benen, Jacques A E ^a   Kester, Harry C M ^b   Visser, Jaap ^b  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b NONE

Author keywords

Aspergillus niger; Endopolygalacturonase; Kinetics; Processivity; Subsites

Indexed keywords

PECTIN; POLYGALACTURONASE; POLYGALACTURONIC ACID;

ARTICLE; ASPERGILLUS NIGER; ENZYME ACTIVITY; ENZYME ISOLATION; ENZYME MECHANISM; ENZYME POLYMORPHISM; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ESTERIFICATION; NONHUMAN; PRIORITY JOURNAL;

ASPERGILLUS NIGER; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; ISOENZYMES; KINETICS; PECTINS; POLYGALACTURONASE; POLYSACCHARIDE-LYASES; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

ASPERGILLUS NIGER;

EID: 0033083712     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00080.x     Document Type: Article

References (24)

1. 1. Bussink, H.J.D., Buxton, F.P., Fraaye, B.A., de Graaff, L.H. & Visser, J. (1992) The polygalacturonases of Aspergillus niger are encoded by a family of diverged genes. Eur. J. Biochem. 208, 83-90.
2. 2. Bussink, H.J.D., Kester, H.C.M. & Visser, J. (1990) Molecular cloning, nucleotide sequence and expression of the gene encoding prepropolygalacturonase II of Aspergillus niger. FEBS Lett. 273, 127-130.
3. 3. Bussink, H.J.D., Brouwer, K.B., de Graaff, L.H., Kester, H.C.M. & Visser, J. (1991) Identification and characterization of a second polygalacturonase gene of Aspergillus niger. Curr. Genet. 20, 301-307.
4. 4. Bussink, H.J.D., van den Hombergh, J.P.T.W., van den Ijssel, P.R.L.A. & Visser, J. (1992) Characterization of polygalacturonase-overproducing Aspergillus niger transformants. Appl. Microbiol. Biotechnol. 37, 324-329.
5. 5. Pařenicová, L., Benen, J.A.E., Kester, H.C.M. & Visser, J. (1998) pgaE encodes a fourth member of the endopolygalacturonase gene family from Aspergillus niger. Eur. J. Biochem. 251, 72-80.
6. 6. Rexová-Benková, L. (1973) The size of the substrate binding site of an Aspergillus niger extracellular endopolygalacturonase. Eur. J. Biochem. 39, 109-115.
7. 7. Rexová-Benková, L. & Mracková, M. (1978) Active groups of an extracellular endo-D-galacturonase of Aspergillus niger derived from pH effect and kinetic data. Biochim. Biophys. Acta 523, 162-169.
8. 8. Kester, H.C.M. & Visser, J. (1990) Purification and characterization of polygalacturonases produced by the hyphal fungus Aspergillus niger. Biotechnol. Appl. Biochem. 12, 150-160.
9. 9. Sambrook, J., Fritsch, E.F. & Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
10. 10. de Graaff, L.H., van den Broeck, H.C. & Visser, J. (1992) Isolation and characterization of the Aspergillus niger pyruvate kinase gene. Curr. Genet. 22, 21-27.
11. 11. Kusters-van Someren, M.A., Harmsen, J.A.M., Kester, H.C.M. & Visser, J. (1991) Structure of the Aspergillus niger pelA gene, its expression in Aspergillus niger and Aspergillus nidulans. Curr. Genet. 20, 293-299.
12. 12. Edelhoch, H. (1967) Spectroscopic determination of tryptophan and tyrosine in proteins. Biochemistry 6, 1948-1954.
13. 13. Kester, H.C.M., Kusters-van Someren, M.A., Müller, Y. & Visser, J. (1996) Primary structure and characterization of an exopolygalacturonase from Aspergillus tubingensis. Eur. J. Biochem. 240, 738-746.
14. 14. Hiromi, K., Nitta, Y., Numata, C. & Ono, S. (1973) Subsite affinities of glucoamylase: examination of the validity of the subsite theory. Biochim. Biophys. Acta 302, 362-375.
15. 15. Suganuma, T., Matsuno, R., Ohnishi, M. & Hiromi, K. (1978) A study of the mechanism of action of Taka-amylase A1 on linear oligosaccharides by product analysis and computer simulation. J. Biochem. (Tokyo) 84, 293-316.
16. 16. Thoma, J.A., Brothers, C. & Spradlin, J. (1970) Subsite mapping of enzymes. Studies on Bacillus subtilis amylase. Biochemistry 9, 1768-1775.
17. 17. Thoma, J.A., Rao, G.V.K., Brothers, C. & Spradlin, J. (1971) Subsite mapping of enzymes. J. Biol. Chem. 246, 5621-5635.
18. 18. Alan, J.D. & Thoma, J.A. (1976) Subsite mapping of enzymes. Depolymerase computer modelling. Biochem. J. 159, 105-120.
19. 19. Alan, J.D. & Thoma, J.A. (1976) Subsite mapping of enzymes. Application of the depolymerase computer model to two α-amylases. Biochem. J. 159, 121-132.
20. 20. Moldano, M.C., Strasser de Saad, M.A. & Callieri, D. (1989) Catabolite repression of the synthesis of inducible polygalacturonase and pectinesterase by Aspergillus niger sp. Curr. Microbiol. 18, 303-306.
21. 21. Biely, P., Benen, J.A.E., Heinrichová, K., Kester, H.C.M. & Visser, J. (1996) Inversion of configuration during hydrolysis of alpha-1,4-galacturonidic linkage by three Aspergillus polygalacturonases. FEBS Lett. 382, 249-255.
22. 22. Henrissat, B. (1991) A classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 280, 309-316.
23. 23. Robyt, J.F. & French, D. (1967) Multiple attack hypothesis of α-amylase action: action of porcine pancreatic, human salivary, and Aspergillus orizae α-amylases. Arch. Biochem. Biophys. 122, 8-16.
24. 24. Robyt, J.F. & French, D. (1970) Multiple attack and polarity of action of porcine pancreatic α-amylase. Arch. Biochem. Biophys. 138, 662-670.