Production of reagents and optimization of methods for studying calmodulin-binding proteins

Protein Expression and Purification

Volumn 15, Issue 1, 1999, Pages 24-33

  Ulbricht, Bettina ^a   Soldati, Thierry ^a  

^a MAX PLANCK INSTITUTE FOR MEDICAL RESEARCH   (Germany)

Author keywords

Affinity chromatography; Calmodulin; Calmodulin binding proteins; Dictyostelium discoideum; Myosins

Indexed keywords

AFFINITY CHROMATOGRAPHY; DICTYOSTELIUM DISCOIDEUM; ESCHERICHIA COLI; IMMUNOFLUORESCENCE; IMMUNOPRECIPITATION; PROTEIN BINDING; PROTEIN PURIFICATION; WESTERN BLOTTING;

DICTYOSTELIUM DISCOIDEUM; ESCHERICHIA COLI;

EID: 0033082673     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0983     Document Type: Article

References (39)

1. Klee C. B., Vanaman T. C. Calmodulin. Adv. Protein Chem. 35:1982;213-321.
2. Burgess W. H., Schleicher M., Van Eldik L. J., Waterson D. M. Calcium and Cell Function. 1983;Academic Press, New York. p. 209-261.
3. Marshak D. R., Clarke M., Roberts D. M., Watterson D. M. Structural and functional properties of calmodulin from the eukaryotic microorganismDictyostelium discoideum. Biochemistry. 23:1984;2891-2899.
4. Liu T. Y., Williams J. G., Clarke M. Inducible expression of calmodulin antisense RNA inDictyostelium. Mol. Biol. Cell. 3:1992;1403-1413.
5. Lydan M. A., Cotter D. A. Spore swelling inDictyostelium. FEMS Microbiol. Lett. 115:1994;137-142.
6. Lydan M. A., O'Day D. H. Different developmental functions for calmodulin inDictyostelium: Exp. Cell Res. 178:1988;51-63.
7. Zhu Q. L., Clarke M. Association of calmodulin and an unconventional myosin with the contractile vacuole complex ofDictyostelium discoideum. J. Cell Biol. 118:1992;347-358.
8. Hammer J., Lydan M., Jung G. Dictyostelium. Mol. Biol. Cell. 7:1996;374a.
9. Wolenski J. S. Regulation of calmodulin binding myosins. Trends Cell Biol. 5:1995;310-316.
10. Studier F. W., Rosenberg A. H., Dunn J. J., Dubendorff J. W. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:1990;60-89.
11. 2+. Biochem. Biophys. Res. Commun. 104:1982;830-836.
12. Neuhaus E., Horstmann H., Almers W., Maniak M., Soldati T. Ethane-freezing/methanol-fixation of cell monolayers: A procedure for improved preservation of structure and antigenicity for light and electron microscopies. J. Struct. Biol. 121:1998;326-342.
13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
14. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
15. Dammann H., Hellstern S., Husain Q., Mutzel R. Primary structure, expression and developmental regulation of aDictyostelium. Eur. J. Biochem. 238:1996;391-399.
16. Ruppert C., Kroschewski R., Bahler M. ) Identification, characterization and cloning of myr 1, a mammalian myosin-I. J. Cell Biol. 120:1993;1393-1403.
17. Sussmann M. Cultivation and synchronous morphogenesis ofDictyostelium discoideum. Methods Cell Biol. 28:1987;9-29.
18. Kerboeuf D., Le Berre A., Dedieu J. C., Cohen J. Calmodulin is essential for assembling links necessary for exocytotic membrane fusion inParamecium. EMBO J. 12:1993;3385-3390.
19. Kink J. A., Maley M. E., Ling K. Y., Kanabrocki J. A., Kung C. Efficient expression of theParameciumEscherichia coli. J. Protozool. 38:1991;441-447.
20. Clarke M., Bazari W. I., Kayman S. C. Isolation and properties of calmodulin fromDictyostelium discoideum. J. Bacteriol. 141:1980;397-400.
21. Bazari W. L., Clarke M. Dictyostelium. Cell Motil. 2:1982;471-482.
22. 32. BioTechniques. 21:1996;292-296.
23. 2+. Proc. Natl. Acad. Sci. USA. 76:1979;204-208.
24. Larson R. E., Pitta D. E., Ferro J. A. A novel 190 kDa calmodulin-binding protein associated with brain actomyosin. Braz. J. Med. Biol. Res. 21:1988;213-217.
25. Snedden W. A., Koutsia N., Baum G., Fromm H. Activation of a recombinant petunia glutamate decarboxylase by calcium/calmodulin or by a monoclonal antibody which recognizes the calmodulin binding domain. J. Biol. Chem. 271:1996;4148-4153.
26. D'Sa C., Arthur R., Jennings I., Cotton R. G., Kuhn D. M. Tryptophan hydroxylase: Purification by affinity chromatography on calmodulin-sepharose. J. Neurosci. Methods. 69:1996;149-153.
27. Cohen P., Klee C. B. Calmodulin: Molecular Aspects of Cellular Regulation. 1988;Elsevier, Amsterdam.
28. Rhoads A. R., Friedberg F. Sequence motifs for calmodulin recognition. FAESB J. 11:1997;331-340.
29. Sonnemann J., Bauerle A., Winckler T., Mutzel R. A ribosomal calmodulin-binding protein fromDictyostelium. J. Biol. Chem. 266:1991;23091-23096.
30. Martoglio B., Graf R., Dobberstein B. Signal peptide fragments of preprolactin and HIV-1 p-gp160 interact with calmodulin. EMBO J. 16:1997;6636-6645.
31. Zhu Q. L., Liu T. Y., Clarke M. Calmodulin and the contractile vacuole complex in mitotic cells ofDictyostelium discoideum. J. Cell Sci. 104:1993;1119-1127.
32. Peterson M. D., Urioste A. S., Titus M. A. Dictyostelium discoideum. J. Muscle Res. Cell Motil. 17:1996;411-424.
33. Hammer J. A., Jung G. The sequence of theDictyostelium. J. Biol. Chem. 271:1996;7120-7127.
34. Colowick S. P., Kaplan N. O. Means A. R., Conn P. M. Methods in Enzymology. 1987;Academic Press, New York.
35. Reddy A. S., Narasimhulu S. B., Safadi F., Golovkin M. A plant kinesin heavy chain-like protein is a calmodulin-binding protein. Plant J. 10:1996;9-21.
36. Kincaid R. L., Billingsley M. L., Vaughan M. Preparation of fluorescent, cross-linking, and biotinylated calmodulin derivatives and their use in studies of calmodulin-activated phosphodiesterase and protein phosphatase. Methods Enzymol. 159:1988;605-626.
37. Szymanska G., O'Connor M. B., O'Connor C. M. Construction of an epitope-tagged calmodulin useful for the analysis of calmodulin-binding proteins: Addition of a hemagglutinin epitope does not affect calmodulin-dependent activation of smooth muscle myosin light chain kinase. Anal. Biochem. 252:1997;96-105.
38. Kincaid R. L., Vaughan M. Purification and properties of calmodulin-activated cyclic nucleotide phosphodiesterase from mammalian brain. Methods Enzymol. 159:1988;557-573.
39. Baehler M., Kroschewski R., Stoffler H. E., Behrmann T. Rat myr 4 defines a novel subclass of myosin I: Identification, distribution, localization, and mapping of calmodulin-binding sites with differential calcium sensitivity. J. Cell Biol. 126:1994;375-389.