Detection of calmodulin-binding proteins using a 32P-Labeled GST- calmodulin fusion protein and a novel renaturation protocol

BioTechniques

Volumn 21, Issue 2, 1996, Pages 292-296

  Fischer, Roland ^a   Wei, Yu ^a   Berchtold, Martin ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

CALMODULIN; CALMODULIN BINDING PROTEIN; CYCLIC AMP DEPENDENT PROTEIN KINASE; EPITOPE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PHOSPHORUS 32;

ARTICLE; GOOD LABORATORY PRACTICE; ISOTOPE LABELING; PROTEIN DENATURATION; PROTEIN DETERMINATION; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS);

EID: 0029760620     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96212rr03     Document Type: Article

References (10)

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2. 125I-containing acylating agent. Biochem. J. 133:529-539.
3. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
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5. Kawasaki, H. and R.H. Kretsinger. 1994. Calcium-binding proteins. 1. EF-hands. Protein Profile 1:343-517.
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7. Nojima, H. 1989. Structural organization of multiple rat calmodulin genes. J. Mol. Biol. 208:269-282.
8. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
9. 125I-Labeled protein gel overlay technique to study calmodulin-binding proteins, p. 433-444. In A.R. Means and P.M. Conn (Eds.), Methods in Enzymology, Vol. 139. Academic Press, New York.
10. Stirling, D.A., A. Petrie, D.J. Pulford, D.T. Paterson and M.J. Stark. 1992. Protein A-calmodulin fusions: a novel approach for investigating calmodulin functions in yeast. Mol. Microbiol. 6:703-713.