Cardioprotective effect of α-tocopherol, ascorbate, deferoxamine, and deferiprone: Mitochondrial function in cultured, iron-loaded heart cells

Journal of Laboratory and Clinical Medicine

Volumn 133, Issue 2, 1999, Pages 179-188

  Link, Gabriela ^b   Konijn, Abraham M ^b   Hershko, Chaim ^a  

^a SHAARE ZEDEK MEDICAL CENTER   (Israel)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0033082143     PISSN: 00222143     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-2143(99)90011-2     Document Type: Article

References (46)

1. 1. Gabutti V, Borgna-Pignatti C. Clinical manifestations and therapy of transfusional haemosiderosis. Clin Haematol 1996;7:919-40.
2. 2. Moreb J, Hershko C, Hasin Y. Effects of acute iron loading on contractility and spontaneous beating rate of cultured rat myocardial cells. Basic Res Cardiol 1988;83:360-8.
3. 3. Link G, Athias P, Grynberg A, Pinson A, Hershko C. Effect of iron loading on transmembrane potential, contraction and automaticity of rat ventricular muscle cells in culture. J Lab Clin Med 1989;113:103-11.
4. 4. Link G, Pinson A, Hershko C. Heart cells in culture: a model of myocardial iron overload and chelation. J Lab Clin Med 1985;106:147-53.
5. 5. Hershko C, Link G, Pinson A. Modification of iron uptake and lipid peroxidation by hypoxia, ascorbic acid, and α-tocopherol in iron-loaded rat myocardial cell cultures. J Lab Clin Med 1987;110:355-61.
6. 6. Link G, Pinson A, Kahane I, Hershko C. Iron loading modifies the fatty acid composition of cultured rat myocardial cells and liposomal vesicles: effect of ascorbate and α-tocopherol on myocardial lipid peroxidation. J Lab Clin Med 1989;114:243-9.
7. 7. Hershko C, Link G, Pinson A, Peter HH, Dobbin P, Hider RC. Iron mobilization from myocardial cells by 3-hydroxypyridin-4-one chelators: studies in rat heart cells in culture. Blood 1991;77:2049-53.
8. 8. Link G, Athias P, Grynberg A, Hershko C, Pinson A. Iron loading modifies β-adrenergic responsiveness of cultured ventricular myocytes. Cardioscience 1991;2:27-30.
9. 9. Link G, Pinson A, Hershko C. Iron loading of cultured cardiac myocytes modifies sarcolemmal structure and increases lysosomal fragility. J Lab Clin Med 1993;121:127-34.
10. 10. Link G, Pinson A, Hershko C. The ability of orally effective iron chelators dimethyl-and diethyl-hydroxypyrid-4-one and of deferoxamine to restore sarcolemmal thiolic enzyme activity in iron-loaded heart cells. Blood 1994;83:2692-7.
11. 11. Link G, Saada A, Pinson A, Konijn AM, Hershko C. Pathogenesis of transfusional iron overload: identification of heart mitochondrial respiratory enzymes as a major target of iron toxicity. J Lab Clin Med 1998;131:466-74.
12. 12. Link G, Tirosh R, Pinson A, Hershko C. Role of iron in the potentiation of anthracycline toxicity: identification of heart cell mitochondria as the site of iron-anthracycline interaction. J Lab Clin Med 1996;127:272-8.
13. 13. Mode EB. Elements of statistics. 3rd ed. Englewood Cliffs, NJ: Prentice-Hall Inc; 1961.
14. 14. Hall RE, Henriksson KG, Lewis SF, Haller R, Kennaway NG. Mitochondrial myopathy with succinate dehydrogenase and aconitase deficiency: abnormalities of several iron-sulfur proteins. J Clin Invest 1993;92:2660-6.
15. 15. Singer TP. Determination of the activity of succinate, NADH, choline and a-glycerophosphate dehydrogenases. In: Glick D, editor. Methods of biochemical analysis (vol 22). New York: Interscience; 1974. pp 123-75.
16. 16. Sottocasa GL, Kuylenstierna B, Ernster L, Bergstrand A. An electron transport system associated with the outer membrane of liver mitochondria. J Cell Biol 1967;32:415-38.
17. 17. Hatefi Y, Stigall DL. Preparation and properties of succinate: ubiquinone oxidoreductase (complex II). Methods Enzymol 1978;53:21-7.
18. 18. Fisher JC, Ruitenbeek W, Trijbels JMF, Veerkamp JH, Stadhouders AM, Sengers RCA, et al. Estimation of NADH oxidation in human skeletal muscle mitochondria. Clin Chim Acta 1986;155:263-74.
19. 19. Trumpower BL, Edwards CA. Purification of a reconstitutively active iron sulfur protein (oxidation factor) from succinate-cytochrome c reductase complex of bovine heart mitochondria. J Biol Chem 1979;254:8697-706.
20. 20. Birch-Machin MA, Briggs HL, Sasorido AA, Bindoff LA, Turnbull DM. An evaluation of the measurement of the activities of complexes I-IV in the respiratory chain of human skeletal muscle mitochondria. Biochem Med Metabol Biol 1994;51:35-42.
21. 21. Wharton DC, Tzagaloff A. Cytochrome C oxidase from beef heart mitochondria. Methods Enzymol 1967;10:245-6.
22. 22. Srere PA. Citrate synthase. Methods Enzymol 1969;13:3-11.
23. 23. Bacon BR, O'Neill R, Britton RS. Hepatic mitochondrial energy production in rats with chronic iron overload. Gastroenterology 1993;105:1134-40.
24. 24. Zhang Y, Marcillat O, Giulivi C, Ernster L, Davies KJ. The oxidative inactivation of mitochondrial electron transport chain components and ATPase. J Biol Chem 1990;265:16330-6.
25. 25. Reynolds LG. Diagnosis and management of acute iron poisoning. Clin Haematol 1989;2:423-33.
26. 26. Brittenham GM, Bergeron RJ. Effects of iron chelators on iron metabolism in iron overload. Molecular Mechanisms of Iron Homeostasis in Health and Disease. Research Workshop of the Israel Science Foundation. The Hebrew University of Jerusalem, May 17-21, 1998. Abstract 7.
27. 27. Fitchett DH, Coltart DJ, Littler WA, Leyland MJ, Trueman T, Gozzard DI, et al. Cardiac involvement in secondary haemochromatosis: a catheter biopsy study and analysis of myocardium. Cardiovasc Res 1980;14:719-24.
28. 28. Gal M, Link G, Hershko C, Konijn AM. Regulation of ferritin synthesis in iron loaded rat cardiomyocyte cells in culture. American Society of Hematology, Fortieth Annual Meeting. December 4-8, Miami Beach, FL. Blood (in press).
29. 29. Hider RC, Choudhury R, Rai BL, Dehkordi LS, Singh S. Design of orally active iron chelators. Acta Haematologica 1996;95:6-12.
30. 30. Hershko C, Konijn AM, Link G. Iron chelators for thalassaemia. Br J Haematol 1998;101:399-406.
31. 31. Grady RW, Hilgartner MW, Giardina PJ. Deferiprone: its efficacy relative to that of desferal. Thirty-Eighth Annual Meeting, American Society of Hematology. Orlando, FL. Blood 1996;suppl 1:abstract 1230.
32. 32. Iancu TC, Shiloh H, Link G, Bauminger ER, Pinson A, Hershko C. Ultrastructural pathology of iron loaded rat myocardial cells in culture. Br J Exp Pathol 1987;68:53-65.
33. 33. Halliwell B, Gutteridge JMC. Role of free radicals and catalytic metal ions in human disease: an overview. Methods Enzymol 1990;186:1-85.
34. 34. Heys AD, Dormandy TL. Lipid peroxidation in iron loaded spleens. Clin Sci 1981;60:295-301.
35. 35. O'Connell MJ, Ward RJ, Baum H, Peters TJ. The role of iron in ferritin- and haemosiderin-mediated lipid peroxidation in liposomes. Biochem J 1985;229:135-9.
36. 36. Hershko C, Weatherall DJ. Iron chelating therapy. Crit Rev Clin Lab Sci 1988;26:303-45.
37. 37. Nienhuis AW. Vitamin C and iron. N Engl J Med 1981;304:170-1.
38. 38. Buettner GR, Jurkiewicz A. Catalytic metals, ascorbate and free radicals: combinations to avoid. Radiat Res 1996;145:532-41.
39. 39. Hershko C. Mechanism of iron toxicity and its possible role in red cell membrane damage. Semin Hematol 1989;26:277-85.
40. 40. Rachmilewitz EA, Shifter A, Kahane I. Vitamin E deficiency in α-thalassemia major: changes in hematological and biochemical parameters after therapeutic trial with α-tocopherol. Am J Clin Nutr 1979;32:1850-9.
41. 41. Cecil. Textbook of medicine. Bennett JC, Plum F, editors. 20th ed. Philadelphia: WB Saunders; 1996. p. 2228.
42. 42. Dresow B, Albert C, Zimmermann I, Nielsen P. Ethane exhalation and vitamin E/ubiquinol status as markers of lipid peroxidation in ferrocene iron-loaded rats. Hepatology 1995;21:1099-105.
43. 43. Omara FO, Blakley BR. Vitamin E is protective against iron toxicity and iron-induced hepatic vitamin E depletion in mice. J Nutr 1993;123:1649-55.
44. 44. Janero DR, Burghardt B. Oxidative injury to myocardial membrane: direct modulation by endogenous alpha-tocopherol. J Mol Cell Cardiol 1989;11:1111-24.
45. 45. Sharma BK, Bacon BR, Britton RS, Park CH, Magiera CJ, O'Neill R, et al. Prevention of hepatocyte injury and lipid peroxidation by iron chelators and alpha-tocopherol in isolated iron-loaded rat hepatocytes. Hepatology 1990;12:31-9.
46. 46. Lindvall S, Ryden L, Smedgard P, Swedberg B. Alpha-toco-pherol and cardiac toxicity of iron. Scand J Haematol 1980;25:331-8.