Nucleoside transport in human colonic epithelial cell lines: Evidence for two Na+-independent transport systems in T84 and Caco-2 cells1

Biochimica et Biophysica Acta - Biomembranes

Volumn 1419, Issue 1, 1999, Pages 15-22

  Ward, Jeffrey L ^a   Tse, C Ming ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Colonic epithelial cell; Nucleoside transport; Reverse transcription polymerase chain reaction

Indexed keywords

CARRIER PROTEIN; DIPYRIDAMOLE; MESSENGER RNA; NITROBENZYLTHIOINOSINE; RNA; SODIUM ION; TRITIUM; URIDINE;

ARTICLE; CELL LINE; CELL STRAIN CACO 2; COLON MUCOSA; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM CELL; MONOLAYER CULTURE; NUCLEOSIDE TRANSPORT; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

CACO-2 CELLS; CARRIER PROTEINS; COLON; EPITHELIAL CELLS; EQUILIBRATIVE NUCLEOSIDE TRANSPORTER 1; EQUILIBRATIVE-NUCLEOSIDE TRANSPORTER 2; HUMANS; MEMBRANE PROTEINS; NUCLEOSIDE TRANSPORT PROTEINS; THIOINOSINE;

EID: 0033058435     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0005-2736(99)00045-0     Document Type: Article

References (23)

1. A.M. Mackinnon, D.J. Deller, Purine nucleotide biosynthesis in gastrointestinal mucosa, Biochim. Biophys. Acta 319 (1973) 1-4.
2. S.L. Betcher, J.N. Forrest Jr., R.G. Knickelbein, J.W. Dobbins, Sodium-adenosine cotransport in brush-border membranes from rabbit ileum, Am. J. Physiol. 259 (1990) G504-G510.
3. +-independent nucleoside transporters in Xenopus laevis oocytes, Biochem. Pharmacol. 53 (1997) 1909-1918.
4. S.M. Jarvis, Characterization of sodium-dependent nucleoside transport in rabbit intestinal brush-border membrane vesicles, Biochim. Biophys. Acta 979 (1989) 132-138.
5. S.D. Patil, J.D. Unadkat, Sodium-dependent nucleoside transport in the human intestinal brush-border membrane, Am. J. Physiol. 272 (1997) G1314-G1320.
6. K.J. Tally, B.J. Hrnjez, J.A. Smith, E.C. Mun, J.B. Matthews, Adenosine scavenging : a novel mechanism of chloride secretory control in intestinal epithelial cells, Surgery 120 (1996) 248-254.
7. E.C. Mun, K.J. Tally, J.B. Matthews, Characterization and regulation of adenosine transport in T84 intestinal epithelial cells, Am. J. Physiol. 274 (1998) G261-G269.
8. +-nucleoside cotransporter (hCNT1), Am. J. Physiol. 272 (1997) C707-C714.
9. +-dependent purine nucleoside transporter from human kidney: cloning and functional characterization, Am. J. Physiol. 273 (1997) F1058-F1065.
10. +-dependent nucleoside transporters, Pharm. Res. 14 (1997) 1524-1532.
11. C.R. Crawford, D.H. Patel, C. Naeve, J.A. Belt, Cloning of the human equilibrative, nitrobenzylmercaptopurine riboside (NBMPR)-insensitive nucleoside transporter ei by functional expression in a transporter-deficient cell line, J. Biol. Chem. 273 (1998) 5288-5293.
12. M. Griffiths, S.Y.M. Yao, F. Abidi, S.E.V. Phillips, C.E. Cass, J.D. Young, S.A. Baldwin, Molecular cloning and characterization of a nitrobenzylthioinosine-insensitive (ei) equilibrative nucleoside transporter from human placenta, Biochem. J. 328 (1997) 739-743.
13. M. Griffiths, N. Beaumont, S.Y.M. Yao, M. Sundaram, C.E. Boumah, A. Davies, F.Y.P. Kwong, I. Coe, C.E. Cass, J.D. Young, S.A. Baldwin, Cloning of a human nucleoside transporter implicated in the cellular uptake of adenosine and chemotherapeutic drugs, Nature Med. 3 (1997) 89-93.
14. Q.Q. Huang, S.Y. Yao, M.W. Ritzel, A.R. Paterson, C.E. Cass, J.D. Young, Cloning and functional expression of a complementary DNA encoding a mammalian nucleoside transport protein, J. Biol. Chem. 269 (1994) 17757-17760.
15. +-nucleoside cotransporter, J. Biol. Chem. 270 (1995) 13596-13599.
16. M.J. Hu, Comparison of uptake characteristics of thymidine and zidovudine in a human intestinal epithelial model system, J. Pharm. Sci. 82 (1993) 829-833.
17. J.A. Belt, N.M. Marina, D.A. Phelps, C.M. Crawford, Nucleoside transport in normal and neoplastic cells, Adv. Enzyme Regul. 33 (1993) 235-252.
18. J.B. Williams, A.A. Lanahan, A mammalian delayed-early response gene encodes HNP36, a novel, conserved nucleolar protien, Biochem. Biophys. Res. Commun. 213 (1995) 325-333.
19. S.M. Yao, A.M.L. Ng, W.R. Muzyka, M. Griffiths, C.E. Cass, S.A. Baldwin, J.D. Young, Molecular cloning and functional characterization of nitrobenzylthioinosine (NBMPR)-sensitive (es) and NBMPR-insensitive (ei) equilibrative nucleoside transporter proteins (rENT1 and rENT2) from rat tissues, J. Biol. Chem. 272 (1997) 28423-28430.
20. Y. He, I.R. Sanderson, W.A. Walker, Uptake, transjport and metabolism of exogenous nucleosides in intestinal epithelial cell cultures, J. Nutr. 124 (1994) 1942-1949.
21. M.J. Engle, G.S. Goetz, D.H. Alpers, Caco-2 cells express a combination of colonocyte and enterocyte phenotypes, J. Cell Physiol. 174 (1998) 362-369.
22. +-dependent glucose transporter (SGLT1), J. Biol. Chem. 271 (1996) 7738-7744.
23. G.R. Strohmeier, S.M. Reppert, W.I. Lencer, J.L. Madara, The A2b adenosine receptor mediates cAMP responses to adenosine receptor agonists in human intestinal epithelia, J. Biol. Chem. 270 (1995) 2387-2394.