Surface-modified mutants of cytochrome P450(cam): Enzymatic properties and electrochemistry

FEBS Letters

Volumn 451, Issue 3, 1999, Pages 342-346

  Lo, Kenneth Kam Wing ^a   Wong, Luet Lok ^a   Hill, H Allen O ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

Author keywords

Electrochemistry; Mutagenesis; P450(cam); Redox active label; Surface cysteine

Indexed keywords

CAMPHOR; CAMPHOR 5 MONOOXYGENASE; CYSTEINE; FERROCENE; HEME; MUTANT PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ELECTROCHEMISTRY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME STRUCTURE; GENETIC ENGINEERING; GENETIC VARIABILITY; PRIORITY JOURNAL;

ANIMALS; CAMPHOR 5-MONOOXYGENASE; ELECTROSTATICS; ENZYME ACTIVATION; ESCHERICHIA COLI; MUTATION; RECOMBINANT PROTEINS;

DRYOBALANOPS;

EID: 0033057343     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(99)00611-0     Document Type: Article

References (31)

1. Ortiz de Montellano P.R. (Ed.) (1995) Cytochrome P450: Structure, Mechanism, and Biochemistry, 2nd edn., Plenum Press, New York.
2. Yu C.A., Gunsalus I.C. J. Biol. Chem. 249:1974;94-101.
3. Sligar S.G. Biochemistry. 15:1976;5399-5406.
4. Sligar S.G., Gunsalus I.C. Proc. Natl. Acad. Sci. USA. 73:1976;1078-1802.
5. Peterson J.A. Arch. Biochem. Biophys. 144:1971;678-693.
6. Sligar S.G., Cinti D.L., Gibson G.G., Schenkman J.B. Biochem. Biophys. Res. Commun. 90:1979;925-932.
7. Kazlauskaite, J., Westlake, A.C.G., Wong, L.L. and Hill, H.A.O. (1996) Chem. Commun. 2189-2190.
8. Zhang Z., Nassar A.E.F., Lu Z., Schenkman J.B., Rusling J.F. J. Chem. Soc. Faraday Trans. 93:1997;1769-1774.
9. Lvov Y.M., Lu Z., Schenkman J.B., Zu X., Rusling J.F. J. Am. Chem. Soc. 120:1998;4073-4080.
10. Stayton P.S., Sligar S.G. Biochemistry. 29:1990;7381-7386.
11. Koga H., Sagara Y., Yaoi T., Tsujimura M., Nakamura K., Sekimizu K., Makino R., Shimada H., Ishimura Y., Yura K., Go M., Ikeguchi M., Horiuchi T. FEBS Lett. 331:1993;109-113.
12. Nakamura K., Horiuchi T., Yasukochi T., Sekimizu K., Hara T., Sagara Y. Biochim. Biophys. Acta. 1207:1994;40-48.
13. Unno M., Shimada H., Toba Y., Makino R., Ishimura Y. J. Biol. Chem. 271:1996;17869-17874.
14. Stayton P.S., Poulos T.L., Sligar S.G. Biochemistry. 28:1989;8201-8205.
15. Pochapsky T.C., Lyons T.A., Kazanis S., Arakaki T., Ratnaswamy G. Biochimie. 78:1996;723-733.
16. Poulos T.L., Finzel B.C., Howard A.J. J. Mol. Biol. 195:1987;687-700.
17. Vigmond S.J., Iwakura M., Mizutani F., Katsura T. Langmuir. 10:1994;2860-2862.
18. Brizzolara R.A., Boyd J.L., Tate A.E. J. Vac. Sci. Technol. A. 15:1997;773-778.
19. Nickerson D.P., Wong L.L. Protein Eng. 10:1997;1357-1361.
20. Sambrook, J., Fritsch, E.F. and Maniatas, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
21. Kunkel T.A. Proc. Natl. Acad. Sci. USA. 82:1985;488-492.
22. Unger B.P., Gunsalus I.C., Sligar S.G. J. Biol. Chem. 261:1986;1158-1163.
23. Yasukochi T., Okada O., Hara T., Sagara Y., Sekimizu K., Horiuchi T. Biochim. Biophys. Acta. 1204:1994;84-90.
24. Peterson J.A., Lorence M.C., Amarneh B. J. Biol. Chem. 265:1990;6066-6073.
25. Sligar S.G., Filipovic D., Stayton P.S. Methods Enzymol. 206:1991;31-49.
26. Nickerson D.P., Harford-Cross C.F., Fulcher S.R., Wong L.L. FEBS Lett. 405:1997;153-156.
27. Di Gleria K., Hill H.A.O., Wong L.L. FEBS Lett. 390:1996;142-144.
28. Di Gleria K., Nickerson D.P., Hill H.A.O., Wong L.L., Fülöp V. J. Am. Chem. Soc. 120:1998;46-52.
29. Ellman G.L. Arch. Biochem. Biophys. 82:1959;70-77.
30. Fisher M.T., Sligar S.G. J. Am. Chem. Soc. 107:1985;5018-5019.
31. Hill H.A.O., Hunt N.I., Bond A.M. J. Electroanal. Chem. 436:1997;17-25.