Direct determination of carbon and nitrogen contents of natural bacterial assemblages in marine environments

Applied and Environmental Microbiology

Volumn 64, Issue 9, 1998, Pages 3352-3358

  Fukuda, Rumi ^a   Ogawa, Hiroshi ^a   Nagata, Toshi ^a   Koike, Isao ^a  

^a UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON; NITROGEN;

BACTERIUM; BIOMASS; CARBON; ESTIMATION METHOD; MARINE ENVIRONMENT; NITROGEN;

ARTICLE; BACTERIUM DETECTION; CARBON DIOXIDE FIXATION; FLUORESCENCE MICROSCOPY; FOOD CHAIN; MARINE ENVIRONMENT; NITROGEN FIXATION; NONHUMAN; PHYTOPLANKTON; THERMODYNAMICS;

BACTERIA (MICROORGANISMS);

EID: 0031664572     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.64.9.3352-3358.1998     Document Type: Article

References (44)

1. Azam, F., T. Fenchel, J. G. Field, J. S. Gray, L. A. Meyer-Reil, and F. Thingstad. 1983. The ecological role of water-column microbes in the sea. Mar. Ecol. Prog. Ser. 10:257-263.
2. Benner, R. 1991. Ultra-filtration for the concentration of bacteria, viruses, and dissolved organic matter. Gcophys. Monogr. 63:275-280.
3. Billen, G., C. Joiris, L. Meyer-Reil, and H. Lindeboom. 1990. Role of bacteria in the North Sea ecosystem. Neth. J. Sea Res. 4:265-293.
4. Bjørnsen, P. K. 1986. Automatic determination of bacterioplankton biomass by image analysis. Appl. Environ. Microbiol. 51:1199-1204.
5. Bjørnsen, P. K., and B. Riemann. 1988, Towards a quantitative stage in the study of microbial processes in pelagic carbon flows. Ergeb. Limnol. 31:185-193.
6. Bjørnsen, P. K., and J. Kuparinen. 1991. Determination of bacterioplankton biomass, net production and growth efficiency in the Southern Ocean. Mar. Ecol. Prog. Ser. 71:185-194.
7. Bratbak, G. 1985. Bacterial biovolume and biomass estimations. Appl. Environ. Microbiol. 49:1488-1493.
8. Buck, K. R., F. P. Chavez, and L. Campbell. 1996. Basin-wide distributions of living carbon components and the inverted trophic pyramid of the central gyre of the North Atlantic Ocean, summer 1993. Aquat. Microb. Ecol. 10:283-298.
9. Caron, D. A., H. G. Dam, P. Kremer, E. J. Lessard, L. P. Madin, T. C. Malone, J. M. Napp, E. R. Peele, M. R. Roman, and M. J. Youngbluth. 1995. The contribution of microorganisms to paniculate carbon and nitrogen in surface waters of the Sargasso Sea near Bermuda. Deep-Sea Res. 42:943-972.
10. Chisholm, S. W., R. J. Olson, E. R. Zettler, R. Goericke, J. B. Waterbury, and N. A. Welschmeyer. 1988, A novel free-living prochlorophyte abundant in the oceanic euphotic zone. Nature 334:340-343.
11. Cho, B. C., and F. Azam. 1990. Biogeochemical significance of bacterial biomass in the ocean's euphotic zone. Mar. Ecol. Prog. Ser. 63:253-259.
12. Christian, J. R., and D. M. Karl. 1994. Microbial community structure at the U.S.-Joint Global Ocean Flux Study Station ALOHA: inverse methods for estimating biochemical indicator ratios. J. Geophys. Res. 99:14269-14276.
13. Coffin, R. B., D. J. Velinsky, R. Devereux, W. A. Price, and L. Cifuentes. 1990. Stable carbon isotope analysis of nucleic acids to trace sources of dissolved substrates used by estuarine bacteria. Appl. Environ. Microbiol. 56:2012-2020.
14. Cole, J. J., S. Findlay, and M. L. Pace. 1988. Bacterial production in fresh and saltwater ecosystems: a cross-system overview. Mar. Ecol. Prog. Ser. 43:1-10.
15. DeLong, E. F. 1992. Archaea in coastal marine environments. Froc. Natl. Acad. Sci. USA 89:5685-5689.
16. Ducklow, H. W., and C. A. Carlson. 1992. Oceanic bacterial production, p. 113-181. In K. C. Marshall (ed.), Advances in microbial ecology. Plenum Press, New York, N.Y.
17. Fagerbakke, K. M., M. Heldat, and S. Norland. 1996. Content of carbon, nitrogen, oxygen, sulfur and phosphorus in native aquatic and cultured bacteria. Aquat. Microb. Ecol. 10:15-27.
18. Fry, J. C. 1988. Determination of biomass, p. 27-72. In B. Austin (ed.), Methods in aquatic bacteriology. John Wiley & Sons, Inc., New York, N.Y.
19. Fuhrman, J. A., D. E. Comeau, Å. Hagström, and A. M. Chan, 1988. Extraction from natural planktonic microorganisms of DNA suitable for molecular biological studies. Appl. Environ. Microbiol. 54:1426-1429.
20. Fuhrman, J. A., T. D. Sleeter, C. A. Carlson, and L. M. Proctor. 1989. Dominance of bacterial biomass in the Sargasso Sea and its ecological implications. Mar. Ecol. Prog. Ser. 57:207-217.
21. Giovannoni, S. J., E. F. DeLong, T. M. Schmidt, and N. R. Pace. 1990. Tangential flow filtration and preliminary phylogenetic analysis of marine picoplankton. Appl. Environ, Microbiol. 56:2572-2575.
22. Giovannoni, S. J., T. B. Britschgi, C. L. Moyer, and K. G. Field. 1990. Genetic diversity in Sargasso Sea bacterioplankton. Nature 345:60-63.
23. Goericke, R., and N. A. Welschmeyer. 1993. The marine prochlorophyte Prochlorococcus contributes significantly to phytoplankton biomass and primary production in the Sargasso Sea. Deep-Sea Res. 40:2283-2294.
24. Goldman, J. C., D. A. Caron, and M. R. Dennett. 1987, Regulation of gross growth efficiency and ammonium regeneration in bacteria by substrate C:N ratio. Limnol. Oceanogr. 32:1239-1252.
25. Kirchman, D. L., R. G. Keil, M. Simon, and N. A. Welschmeyer, 1993. Biomass and production of heterotrophic bacterioplankton in the oceanic subarctic Pacific. Deep-Sea Res. 40:967-988.
26. Kogure, K., and I. Koike. 1987. Particle counter determination of bacterial biomass in seawater. Appl. Environ. Microbiol. 53:274-277.
27. Koike, L. S. Hara, K. Terauchi, and K. Kogure. 1990. Role of sub-micrometre particles in the ocean. Nature 345:242-244.
28. Kroer, N. 1994. Relationships between biovolume and carbon and nitrogen content of bacterioplankton. FEMS Microbiol. Ecol. 13:217-224.
29. Lee, S., and J. A. Fuhrman. 1987. Relationships between biovolume and biomass of naturally derived marine bacterioplankton. Appl. Environ. Mi-crobiol. 53:1298-1303.
30. Montesinos, E., I. Esteve, and R. Guerrero. 1983. Comparison between direct methods for determination of microbial cell volume: electron micros-copy and electronic particle sizing. Appl. Environ. Microbiol, 45:1651-1658.
31. Moriarty, D. J. W., M. Bianchi, and V. Talbot. 1997. Bacterial productivity and organic matter flux in the Southern Ocean and in the Antarctic inter-mediate water and mode water of the Indian Ocean. Deep-Sea. Res. Part II 44:1005-1015.
32. Monta, R. Y. 1997. Bacteria in olïgotrophic environments-starvation-sur-vival lifestyle, p. 195-197. International Thomson Publishing, New York, N.Y.
33. Nagata, T. 1986. Carbon and nitrogen content of natural planktonic bacteria. I Appl. Environ. Microbiol. 52:28-32.
34. Nagata, T., and Y. Watanabe. 1990. Carbon- and nitrogen-to-volume ratios of bacterioplankton grown under different nutritional conditions. Appl. En-viron. Microbiol. 56:1303-1309.
35. Ogawa, H., R. Fukuda, and I. Koike. Vertical distributions of dissolved organic carbon and nitrogen in the Southern Ocean. Submitted for publication.
36. Porter, K. G., and Y. S. Feig. 1980. The use of DAPI for identifying and counting aquatic microflora. Limnol. Oceanogr. 25:943-948.
37. Sieracki, M. E., E. M. Haugen, and T. L. Cucci. 1995. Overestimation of heterotrophic bacteria in the Sargasso Sea: direct evidence by flow and imaging cytometry. Deep-Sea Res. 42:1399-1409.
38. Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic bacteria. Mar. Ecol. Prog. Ser. 51:201-213.
39. Simon, M., B. C. Cho, and F. Azam. 1992. Significance of bacterial biomass in lakes and the ocean: comparison to phytoplankton biomass and biogeochemical implications. Mar. Ecol. Prog. Ser. 86:103-110.
40. Suzuki, R., and T. Ishimaru. 1990. An improved method for the determi- ' nation of phytoplankton chlorophyll using N,N-dimethylform amide. J. Oceanogr Soc. Jpn. 46:190-194.
41. Viles, C. L., and M. E. Sieracki. 1992. Measurement of marine picoplankton cell size by using a cooled, charge-coupled device camera with image-ana-lyzed fluorescence microscopy. Appl. Environ. Microbiol. 58:584-592.
42. Wangersky, P. J. 1993. Dissolved organic carbon methods: a critical review. Mar. Chem. 41:61-74.
43. Watson, S. W., T. J. Novitsky, H. L. Quinby, and F. W. Valois. 1977. Determination of bacterial number and biomass in the marine environment. Appl. Environ. Microbiol. 33:940-946.
44. Yamasaki, A., H. Fukuda, R. Fukuda, T. Miyajima, T. Nagata, H. Ogawa, and I. Koike. 1998. Suhmicrometer particles in northwest Pacific coastal environments: abundance, size distribution, and biological origins. Limnol. Oceanogr. 43:536-542.