Purification and some properties of pheophorbidase in Chenopodium album

Plant and Cell Physiology

Volumn 40, Issue 1, 1999, Pages 104-108

  Watanabe, Kenji ^a   Ohta, Hiroyuki ^a   Tsuchiya, Tohru ^a   Mikami, Bunzo ^b   Masuda, Tatsuru ^a   Shioi, Yuzo ^c   Takamiya, Ken Ichiro ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b KYOTO UNIVERSITY   (Japan)

^c SHIZUOKA UNIVERSITY   (Japan)

Author keywords

Chenopodium album; Chlorophyll degradation; Chlorophyllase (EC 3.1.1.14); Pheophorbidase; Pheophorbide a; Pyropheophorbide a.

Indexed keywords

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; CARBOXYLPYROPHEOPHORBIDE A; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME LOCALIZATION; ENZYME PURIFICATION; MOLECULAR WEIGHT; PHEOPHORBIDASE; PHEOPHORBIDE A; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS;

CHENOPODIUM ALBUM;

EID: 0033046676     PISSN: 00320781     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.pcp.a029466     Document Type: Article

References (21)

1. Bradford, M.M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
2. Brown, S.B., Hoghton, J.D. and Hendry, G.A.F. (1991) Chlorophyll breakdown. In Chlorophylls. Edited by Scheer, H. pp. 465-489. CRC Press, Boca Raton, FL.
3. Curty, C., Engel, N. and Gossauer, A. (1995) Evidence for a monooxy-genase-catalyzed primary process in the catabolism of chlorophyll. FEBS Lett. 364:41-44.
4. Douce, R. and Joyard, J. (1982) Purification of the chloroplast envelope. In Methods in Chloroplast Molecular Biology. Edited by Ederman, M., Hallick, R.B. and Chua, N-H. pp. 239-256. Elsevier Biochemical Press, Amsterdam.
5. Ginsburg, S., Schellenberg, M. and Matile, P. (1994) Cleavage of chlorophyll-porphyrin. Requirement for reduced ferredoxin and oxygen. Plant Physiol. 105: 545-554.
6. Hasslacher, M., Schall, M., Hayn, M., Griengl, H., Kohlwein, S.D. and Schwab, H. (1995) Molecular cloning of the full-length cDNA of (S)-hydroxynitrile lyase from Hevea brasiliensis: functional expression in Escherichia coli and Saccharomyces serevisiae and identification of an active residue. J. Biol. Chem. 271: 5884-5891.
7. Hinder, B., Schellenberg, M., Rodoni, S., Ginsburg, S., Vogt, E., Martinoia, E., Matile, P. and Hörtensteiner, S. (1996) How plants dispose of chlorophyll catabolites: directly energized uptake of tetrapyrrolic breakdown products into isolated vacuoles. J. Biol. Chem. 271: 27233-27236.
8. Khalyfa, A., Kermasha, S., Marsot, P. and Goetghebeur, M. (1995) Purification and characterization of chlorophyllase from alga Phaeodactylum tricornutum by preparative native electrophoresis. Appl. Biochem. Biotechnol. 53: 11-27.
9. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
10. Matile, P., Hörtensleiner, S., Thomas, H. and Kräutler, B. (1996) Chlorophyll breakdown in senescent leaves. Plant Physiol. 112: 1403-1409.
11. Mühlecker, W., Ongania, K.-H., Kräutler, B., Matile, P. and Hörtensteiner, S. (1997) Tracking down chlorophyll breakdown in plants: elucidation of the constitution of a "fluorescent" chlorophyll metabolite. Angew. Chem. Int. Ed. Engl. 36: 401-404.
12. Porra, R.J. (1997) Recent progress in porphyrin and chlorophyll biosynthesis. Photochem. Photobiol. 65: 492-516.
13. Rodoni, S., Mühlecker, W., Anderl, M., Kräutler, B., Moser, D., Thomas, H., Matile, P. and Hörtensteiner, S. (1997) Chlorophyll breakdown in senescent chloroplasts: cleavage of pheophorbide a in two enzymic steps. Plant Physiol. 115: 669-676.
14. Shimokawa, K. (1982) Hydrophobic chromatographic purification of ethylene-enhanced chlorophyllase from Citrus unshu fruits. Phytochemistry 21: 543-545.
15. Shioi, Y., Watanabe, K. and Takamiya, K. (1996) Enzymatic conversion of pheophorbide a to the precursor of pyro-pheophorbide a in leaves of Chenopodium album. Plant Cell Physiol. 37: 1143-1149.
16. Smith, A.G., Marsh, O. and Elder, G.H. (1993) Investigation of the subcellular location of the tetrapyrrole-biosynthesis enzyme coproporphyrinogen oxidase in higher plants. Biochem J. 292: 503-508.
17. Suzuki, J.Y., Bolivar, D.W. and Bauer, C.E. (1997) Genetic analysis of chlorophyll biosynthesis. Annu. Rev. Genet. 31: 61-89.
18. Tamai, H., Shioi, Y. and Sasa, T. (1979) Studies on chlorophyllase of Chlorella protothecoides IV. Some properties of the purified enzyme. Plant Cell Physiol. 20: 1141-1145.
19. Thomas, H. (1997) Chlorophyll: a symptom and a regulator of plastid development. New Phytol. 136: 163-181.
20. Trebitsh, T., Goldschmidt, E.E. and Riov, J. (1993) Ethylene induces de novo synthesis of chlorophyllase. Proc. Natl. Acad. Sci. USA 90: 9441-9445.
21. Tsuchiya, T., Ohta, H., Masuda, T., Mikami, B., Kita, N., Shioi, Y. and Takamiya, K. (1997) Purification and characterization of chlorophyllase from mature leaves of Chenopodium album. Plant Cell Physiol. 38: 1026-1031.