Etidronate (EHDP) inhibits osteoclastic-bone resorption, promotes apoptosis and disrupts actin rings in isolate-mature osteoclasts

Calcified Tissue International

Volumn 64, Issue 3, 1999, Pages 219-223

  Hiroi Furuya, E ^a   Kameda, T ^a   Hiura, K ^b   Mano, H ^b   Miyazawa, K ^b   Nakamaru, Y ^b   Watanabe Mano, M ^b   Okuda, N ^b   Shimada, J ^a   Yamamoto, Y ^a   Hakeda, Y ^a   Kumegawa, M ^a,c  

^a MEIKAI UNIVERSITY SCHOOL OF DENTISTRY   (Japan)

^b UNIVERSITY OF TOKUSHIMA   (Japan)

^c NONE   (Japan)

Author keywords

Actin rings; Apoptosis; EHDP; Osteoclast

Indexed keywords

ACTIN; ETIDRONIC ACID;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL ISOLATION; CONTROLLED STUDY; NONHUMAN; OSTEOCLAST; OSTEOLYSIS; PRIORITY JOURNAL; RABBIT;

ACTINS; ANIMALS; ANIMALS, NEWBORN; APOPTOSIS; BONE RESORPTION; CELL SEPARATION; CELLS, CULTURED; DENTIN; ETIDRONIC ACID; MICROSCOPY, FLUORESCENCE; MICROTUBULES; OSTEOCLASTS; RABBITS;

EID: 0033044716     PISSN: 0171967X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002239900606     Document Type: Article

References (24)

1. Fleisch H, Russell RGG, Francis MD (1969) Diphosphonates inhibit hydroxyapatite dissolution in vitro and bone resorption in tissue culture and in vivo. Science 165:1262-1264
2. Fleisch H (1993) Bisphosphonates: mechanisms of action and clinical use. In: Mundy GR, Martin TJ (eds) Physiology of bone. Springer, Berlin, p 377
3. Kanis JA (1991) Drug used for treatment of Paget's disease. In: Kanis JA (ed) Pathophysiology and treatment of Paget's disease of bone. Dunitz, London, p 159
4. Fleisch H (1991) Bisphosphonates. Pharmacology and use in the treatment of tumour-induced hypercalcaemic and metastatic bone disease. Drugs 42:919-944
5. Valkema R, Vismans FJ, Papapoulos SE, Pauwels EK, Bijvoet OL (1989) Maintained improvement in calcium balance and bone mineral content in patients with osteoporosis treated with the bisphosphonate APD. Bone Miner 5:183-192
6. Reginster JY, Lecart MP, Deroisy R, Sarlet N, Denis D, Ethgen D, Collette J, Franchimont P (1989) Prevention of postmenopausal bone loss by tiludronate. Lancet 2:1469-1471
7. Storm T, Thamsborg G, Steiniche T, Genant HK, Sorensen OH (1990) Effect of intermittent cyclical etidronate therapy on bone mass and fracture rate in women with postmenopausal osteoporosis. N Engl J Med 322:1265-1271
8. Watts NB, Harris ST, Genant HK, Wasnich RD, Miller PD, Jackson RD, Licata AA, Ross P, Woodson GC, Yanover MJ, Mysiw WJ, Kohse L, Rao MB, Steiger P, Richmond B, Chestnut CH (1990) Intermittent cyclical etidronate treatment of postmenopausal osteoporosis. N Engl J Med 323:73-79
9. 2 MDP) on the calcification and resorption of cartilage and bone in the tibial epiphysis and metaphysis of rats. Calcif Tissue Int 11: 196-214
10. 2MBP-coated bone. Bone Miner 6:33-43
11. Carano A, Teitelbaum SL, Konsek JD, Schlesinger PH, Blair HC (1990) Bisphosphonates directly inhibit the bone resorption activity of isolated avian osteoclasts in vitro. J Clin Invest 85:456-461
12. Selander K, Lehenkari P, Väänlänen HK (1994) The effects of bisphosphonates on the resorption cycle of isolated osteoclasts. Calcif Tissue Int 555:368-375
13. 2 inhibits osteoclastic bone resorption by inducing osteoclast apoptosis. Biochem Biophys Res Commun 220:515-519
14. Hughes DE, Dai A, Tiffee JC, Li HH, Mundy GR, Boyce BF (1996) Estrogen promotes apoptosis of murine osteoclasts mediated by TGF-β. Nature Med 2:1132-1136
15. Kameda T, Mano H, Yuasa T, Mori Y, Miyazawa K, Shiokawa M, Nakamaru Y, Hiroi E, Kameda A, Yang NN, Hakeda Y, Kumegawa M (1997) Estrogen inhibits bone resorption by directly inducing apoptosis of the bone-resorbing osteoclasts. J Exp Med 186:489-495
16. Hughes DE, Wright KR, Uy HL, Sasaki A, Yoneda T, Roodman GD, Mundy GR, Boyce BF (1995) Bisphosphonates promote apoptosis in murine osteoclasts in vivo and in vitro. J Bone Miner Res 10:1478-1487
17. Wyllie AH, Kerr JF, Currie AR (1980) Cell death: the significance of apoptosis. Int Rev Cytol 6:251-306
18. Wyllie AH, Morris RG, Smith AL, Dunlop D (1984) Chromatin cleavage in apoptosis: association with condensed chromatin morphology and dependence on macromolecular synthesis. J Pathol 142:67-77
19. Murakami H, Takahashi N, Sasaki T, Udagawa N, Tanaka S, Nakamura I, Zhang D, Barbier A. Suda T (1995) A possible mechanism of the specific action of bisphosphonates on osteoclasts: tiludronate preferentially affects polarized osteoclasts having ruffled borders. Bone 17:137-144.
20. Vine C, Fleisch H, Guenther HL (1996) Bisphosphonates induce osteoclasts to secrete an inhibitor of osteoclast-mediated resorption. Endocrinology 137:2324-2333
21. Kakudo S, Miyazawa K, Kameda T, Mano H, Mori Y, Yuasa T. Nakamaru I, Shiokawa M, Nagahira K, Tokunaga S, Hakeda Y, Kumegawa M (1996) Isolation of highly enriched rabbit osteoclasts from collagen gels: a new assay system for bone-resorbing activity of mature osteoclasts. J Bone Miner Metab 14:129-136
22. Takada Y, Kusuda M, Hiura K, Sato T, Mochizuki H, Nagao Y, Tomura M, Yahiro M, Hakeda Y, Kawashima H, Kumegawa M (1992) A simple method to assess osteoclastmediated bone resorption using unfractionated bone cells. Bone Miner 17:347-359
23. Kameda T, Ishikawa H, Tsutsui T (1995) Detection and characterization of apoptosis in osteoclasts in vitro. Biochem Biophys Res Commun 207:753-760
24. Walker NI, Harmon BV, Gobe GC, Kerr JF (1988) Patterns of cell death. Methods Achieve Exp Pathol 13:18-54