Improved protocol for using avian red blood cells as substrates for the polymerase chain reaction

BioTechniques

Volumn 26, Issue 6, 1999, Pages 1080-1082

  Bercovich, Dani ^a,b   Plotsky, Yoram ^a   Gruenbaum, Yosef ^b  

^a MIGAL GALILEE TECHNOLOGY CENTER   (Israel)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

DNA POLYMERASE; PRIMER DNA;

ALLELE; ANIMAL CELL; ARTICLE; CHICKEN; CLINICAL PROTOCOL; ERYTHROCYTE; FREEZING; GENE AMPLIFICATION; NONHUMAN; POLYMERASE CHAIN REACTION; TECHNIQUE;

EID: 0033039204     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/99266bm16     Document Type: Article

References (6)

1. Akane, A. 1996. Hydrogen peroxide decomposes the heme compound in forensic specimens and improves the efficiency of PCR. BioTechniques 21:392-394.
2. Akane, A., K. Matsubara, H. Nakamura, S. Takahashi and K. Kimura. 1994. Identification of the heme compound co-purified with deoxyribonucleic acid (DNA) from bloodstains, a major inhibitor of polymerase chain reaction (PCR) amplification. J. Forensic Sci. 39:362-372.
3. Iraqi, F., H. Khatib, G. Shani, A. Davarsi, A. Frumkin, G. Zeitlin, M. Soller, A. Fainsod and Y. Gruenbaum. 1992. CHoxcad locus may influence quantitative traits in chicken. J. Exp. Zool. 263:303-308.
4. Khatib, H., D. Bercovich, T. Ratz, Y. Plotzki, A. Fainsod and Y. Gruenbaum. 1995. Mapping the CdxA gene into a new linkage group in chicken. Anim. Genet. 26:211.
5. Khatib, H. and Y. Gruenbaum. 1996. Chicken red blood cells as a substrate for direct polymerase chain reaction. Anim. Genet. 27:53-54.
6. Khatib, H., N. Sagiv and Y. Gruenbaum. 1998. Fresh and Frozen pools of chicken red blood cells as a substrates for direct polymerase chain reaction. Poult. Sci. 77:902-904.