Hydrogen peroxide decomposes the heme compound in forensic specimens and improves the efficiency of PCR

BioTechniques

Volumn 21, Issue 3, 1996, Pages 392-394

  Akane, Atsushi ^a  

^a KANSAI MEDICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

HEMOGLOBIN; HYDROGEN PEROXIDE;

ARTICLE; DIAGNOSTIC ACCURACY; DNA TEMPLATE; FORENSIC SCIENCE; GENE AMPLIFICATION; GENE ISOLATION; HEMOGLOBIN DETERMINATION; HUMAN; HUMAN CELL; POLYMERASE CHAIN REACTION;

EID: 0029809739     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96213bm10     Document Type: Article

References (9)

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2. Akane, A., K. Matsubara, H. Nakamura, S. Takahashi and K. Kimura. 1994. Purification of highly degraded DNA by gel filtration for PCR. BioTechniques 16:235-238.
3. Akane, A., H. Shiono, K. Matsubara, H. Nakamura, M. Hasegawa and M. Kagawa. 1993. Purification of forensic specimens for the PCR analysis. J. Forensic Sci. 38:691-701.
4. Berg, S. 1975. Leichenzersetzung und Leichenzerstörung, p. 62-106. In B. Mueller (Ed.), Gerichtlichen Medizin. Springer-Verlag, Berlin.
5. Bunn, H.F. and J.H. Jandl. 1968. Exchange of heme among hemoglobins and between hemoglobin and albumin. J. Biol. Chem. 243:465-475.
6. Comey, C.T., B.W. Koons, K.W. Presly, J.B. Smerick, C.A. Sobieralski, D.M. Stanley and F.S. Baechtel. 1994. DNA extraction strategies for amplified fragment length polymorphism analysis. J. Forensic Sci. 39:1254-1269.
7. Jung, J.M., C.T. Comey, D.B. Baer and V. Rudowle. 1991. Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of HLA-DQα gene. Int. J. Legal Med. 104:145-148.
8. Schapira, G., J. Rosa, N. Maleknia and P. Padieu. 1968. Methods of identification of peptides during hemoglobin biosynthesis and measurement of their sequential synthesis, p. 747-769. In L. Grossman and K. Moldave (Eds.), Methods in Enzymology, Vol. 12. Academic Press, New York.
9. Schwerd, W. 1967. Blutgifte, p. 454-460. In A. Ponsold (Ed.), Lehrbuch der Gerichtlichen Medizin für Mediziner und Juristen. Georg Thieme Verlag, Stuttgart.