Identification of calcium binding sites in the trypanosome flagellar calcium-acyl switch protein

Molecular and Biochemical Parasitology

Volumn 101, Issue 1-2, 1999, Pages 61-70

  Maldonado, Rosa A ^a   Mirzoeva, Salida ^b   Godsel, Lisa M ^b   Lukas, Thomas J ^b   Goldenberg, Samuel ^a   Watterson, D Martin ^b   Engman, David M ^b  

^a INSTITUTO OSWALDO CRUZ   (Brazil)

^b NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Calcium; Flagellum; Myristoylation; Recoverin; Trypanosoma

Indexed keywords

CALCIUM; RECOVERIN;

AMINO ACID SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; CALCIUM BINDING; CALCIUM CELL LEVEL; FLAGELLUM; IMMUNOFLUORESCENCE MICROSCOPY; LIGAND BINDING; MUTANT; MYRISTYLATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRYPANOSOMA; TRYPANOSOMA CRUZI;

AMINO ACID SEQUENCE; ANIMALS; BINDING SITES; CALCIUM; CALCIUM-BINDING PROTEINS; EYE PROTEINS; GENE DELETION; GENETIC VECTORS; HIPPOCALCIN; LIPOPROTEINS; MICROSCOPY, FLUORESCENCE; MOLECULAR SEQUENCE DATA; MUTATION; NERVE TISSUE PROTEINS; PROTOZOAN PROTEINS; RECOMBINANT PROTEINS; RECOVERIN; TRYPANOSOMA CRUZI;

PROTOZOA; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 0033039063     PISSN: 01666851     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-6851(99)00055-9     Document Type: Article

References (42)

1. Rasmussen H. The calcium messenger system (1). New Engl. J. Med. 314:1986;1094-1101.
2. Rasmussen H. The calcium messenger system (2). New Engl. J. Med. 314:1986;1164-1170.
3. Lammel E.M., Barbieri M.A., Wilkowsky S.E., Bertini F., Isola E.L. Trypanosoma cruzi: involvement of intracellular calcium in multipication and differentiation. Exp. Parasitol. 83:1996;240-249.
4. Yakuba M.A., Majumder S., Kierszenbaum F. Changes in Trypanosma cruzi infectivity by treatments that affect calcium ion levels. Mol. Biochem. Parasitol. 66:1994;119-125.
5. Moreno S.N., Silva J., Vercesi A.E., Docampo R. Cystolic-free calcium elevation in Trypanosoma cruzi is required for cell invasion. J. Exp. Med. 180:1994;1535-1540.
6. Docampo R., Pignatoro O.P. The inositol phosphate/diacyglycerol signalling pathway in Trypanosoma cruzi. Biochem. J. 275:1991;407-411.
7. 2+storage in acidocalcisomes of Trypanosoma cruzi. Biochem. J. 310:1995;1005-1012.
8. 2+content and expression of an acidocalcisomal calcium pump are elevated in intracellular forms of Trypanosoma cruzi. Mol. Cell. Biol. 18:1998;2309-2323.
9. Chung S.H., Swindle J. Linkage of the calmodulin and ubiquitin loci in Trypanosoma cruzi. Nucleic Acids Res. 18:1990;4561-4569.
10. Orr G.A., Tanowitz H.B., Wittner M. Trypanosoma cruzi: stage expression of calmodulin-binding proteins. Exp. Parasitol. 74:1992;123-133.
11. 2+-calmodulin dependent protein kinase. FEBS. Lett. 337:1994;293-297.
12. 2+-dependent cyclic AMP phosphodiesterase activity in Trypanosoma cruzi. Mol. Biochem. Parasitol. 17:1985;143-153.
13. 2+-binding protein in trypanosomes. J. Biol. Chem. 264:1989;18627-18631.
14. 2+-binding protein gene of Trypanosoma cruzi are present in other members of the Trypanosomatidae family. Exp. Parasitol. 86:1997;200-205.
15. 2+-binding protein of the flagellum of trypanosomatid protozoan parasites. Nucl. Acids. Res. 18:1990;42-52.
16. Porcel B.M., Bontempi E.J., Henriksson J. et al. Trypanosoma rangeli and Trypanosoma cruzi: molecular characterization of genes encoding putative calcium-binding proteins, highly conserved in trypanosomatids. Exp. Parasitol. 84:1996;387-399.
17. Godsel L.M., Tibbetts R.S., Olson C.L., Chaudoir B.M., Engman D.M. Utility of recombinant flagellar calcium binding protein for the serodiagnosis of Trypanosoma cruzi infection. J. Clin. Microbiol. 33:1995;2082-2085.
18. Krautz G.M., Galvao M.C., Cancado J.R., Guevara-Espinoza A., Ouaissi A., Krettli A.U. Use of a 24-kilodalton Trypanosoma cruzi recombinant protein to monitor cure of human Chagas' disease. J. Clin. Microbiol. 33:1995;2086-2090.
19. Ouaissi A., Aguirre T., Plumas-Marty B. et al. Cloning and sequencing of a 24-kDa Trypanosoma cruzi specific antigen released in association with membrane vesicles and defined by a monoclonal antibody. Biol. Cell. 75:1992;11-17.
20. Godsel LM, Engman DM. Flagellar protein localization mediated by a calcium myristoyl/palmitoyl switch mechanism., EMBO J 1999;18:2057-65.
21. Zozulya S., Stryer L. Calcium-myristoyl protein switch. Proc. Natl. Acad. Sci. USA. 89:1992;11569-11573.
22. Kawamura S., Hisatomi O., Kayada S., Tokunaga F., Kuo C.H. Recoverin has S-modulin activity in frog rods. J. Biol. Chem. 268:1993;14579-14582.
23. Ames J.B., Porumb T., Tanaka T., Ikura M., Stryer L. Amino-terminal myristoylation induces cooperative binding of calcium-binding to recoverin. J. Biol. Chem. 270:1995;4526-4533.
24. Flaherty K.M., Zozulya S., Stryer L., McKay D.B. Three-dimensional structure of recoverin, a calcium sensor in vision. Cell. 75:1993;709-716.
25. 2+induced extrusion of the myristoyl group of recoverin. J. Biol. Chem. 270:1995;30909-30913.
26. Calvert P.D., Klenchin V.A., Bownds M.D. Rhodopsin kinase inhibition by recoverin: function of recoverin myristoylation. J. Biol. Chem. 270:1995;24127-24129.
27. Tanaka T., Ames J.B., Harvey T.S., Stryer L., Ikura M. Sequestration of the membrane targeting myristoyl group of recoverin in the calcium-free state. Nature. 376:1995;444-447.
28. Kirchhoff L.V., Neva F.A. Chagas' disease in Latin American immigrants. JAMA. 254:1985;3058-3060.
29. Godsel L.M., Olson C.L., Lacava Z.G.M., Engman D.M. Comparison of the 24 kDa flagellar calcium-binding protein cDNAs of two strains of Trypanosoma cruzi. J. Euk. Microbiol. 42:1995;320-322.
30. Hattori M., Sakaki Y. Dideoxy sequencing method using denatured plasmid templates. Anal. Biochem. 152:1986;232-238.
31. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:1970;680-685.
32. Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamidegels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA. 76:1979;4350-4354.
33. Mirzoeva S, Weigand S, Lukas TJ, Shuvalova L, Anderson WF, Watterson DM., Analysis of functional coupling between calmodulin's calcium-binding and peptide recognition properties. Biochemistry 1999; in press.
34. Tibbetts R.S., Klein K.G., Engman D.M. A rapid method for protein localization in trypanosomes. Exp. Parasitol. 80:1995;572-574.
35. Bairoch A., Bucher P., Hofmann K. The PROSITE database, its status in 1997. Nucleic Acids Res. 25:1997;217-221.
36. Negele J.C., Dotson D.G., Liu W., Sweeney H.L., Putkey J.A. Mutation of the high affinity calcium-binding sites in cardiac troponin C. J. Biol. Chem. 267:1992;825-831.
37. 2+binding to calmodulin and its role in Schizosaccharomyces pombe as revealed by mutagenesis and NMR spectroscopy. J. Biol. Chem. 270:1995;20643-20652.
38. Strynadka N.C., James M.N. Crystal structures of the helix-loop-helix calcium binding proteins. Annu. Rev. Biochem. 58:1989;951-998.
39. Nelson M.R., Chazin W.J. Calmodulin as a calcium sensor. VanEldik L.J., Watterson D.M. Calmodulin and Signal Transduction. 1998;17-64 Academic Press, San Diego.
40. Haiech J., Kilhoffer M.C., Lukas T.J., Craig T.A., Roberts D.M., Watterson D.M. Restoration of the calcium-binding activity of mutant calmodulins toward normal by the presence of a calmodulin binding structure. J. Biol. Chem. 266:1991;3427-3431.
41. z requires two signals. Mol. Biol. Cell. 9:1998;1-14.
42. Simons K., Ikonen E. Functional rafts in cell membranes. Nature. 387:1997;569-572.