Comparative pharmacology of recombinant human M3 and M5 muscarinic receptors expressed in CHO-K1 cells

British Journal of Pharmacology

Volumn 127, Issue 2, 1999, Pages 590-596

  Watson, Nikki ^a   Daniels, Donald V ^a   Ford, Anthony P D W ^a   Eglen, Richard M ^a   Hegde, Sharath S ^a  

^a ROCHE BIOSCIENCE   (United States)

Author keywords

CHO K1 cells; Inositol phosphates accumulation; Muscarinic antagonists; Muscarinic M3 and M5 receptors

Indexed keywords

11 [[4 [4 (DIETHYLAMINO)BUTYL] 1 PIPERIDYL]ACETYL] 5,11 DIHYDRO 6H PYRIDO[2,3 B][1,4]BENZODIAZEPIN 6 ONE; 4 DIPHENYLACETOXY 1 METHYLPIPERIDINE; ATROPINE; CARBACHOL; DARIFENACIN; HIMBACINE; INOSITOL PHOSPHATE; METHOCTRAMINE; MUSCARINIC M3 RECEPTOR; MUSCARINIC M5 RECEPTOR; MUSCARINIC RECEPTOR BLOCKING AGENT; OXYBUTYNIN; PARA FLUOROHEXAHYDROSILADIFENIDOL; PIRENZEPINE; RECOMBINANT PROTEIN; TOLTERODINE; TRITIUM; ZAMIFENACIN;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; RECEPTOR AFFINITY;

ANIMALS; CARBACHOL; CHO CELLS; CRICETINAE; HUMANS; INOSITOL PHOSPHATES; KINETICS; MANDELIC ACIDS; MUSCARINIC AGONISTS; MUSCARINIC ANTAGONISTS; RECEPTOR, MUSCARINIC M3; RECEPTOR, MUSCARINIC M5; RECEPTORS, MUSCARINIC; RECOMBINANT PROTEINS; STIMULATION, CHEMICAL;

EID: 0033036781     PISSN: 00071188     EISSN: None     Source Type: Journal    
DOI: 10.1038/sj.bjp.0702551     Document Type: Article

References (19)

1. Arunlakshana, O. & Schild, H.O. (1959). Some quantitative uses of drug antagonists. Br. J. Pharmacol. Chemother., 14, 48-58.
2. 4 subtype mediates the contraction of the rabbit iris sphincter. Naunyn-Schmiedeberg's Arch. Pharmacol., 345, 611-618.
3. 2 muscarinic receptors on the iris sphincter muscle differ from those on iris noradrenergie nerves. Eur. J. Pharmacol., 163, 263-274.
4. Bolden, C., Cusack, B. & Richelson, E. (1992). Antagonism by antimuscarinic and neuroleptic compounds at the five cloned human muscarinic cholinergic receptors expressed in chinese hamster ovary cells. J. Pharmacol. Exp. Ther., 260, 576-580.
5. Brown, E., Kendall, D.A. & Nahorski, S.R. (1984). Inositiol phospholipid hydrolysis in rat cerebral cortical slices. I. Receptor characterization. J. Neurochem., 42, 1379-1387.
6. Buckley, N.J., Bonner, T.I., Buckley, C.M. & Brann, M.R. (1989). Antagonist binding properties of five cloned muscarinic receptors expressed in CHO-K1 cells. Mol. Pharmacol., 35, 469-476.
7. Caulfield, M.P. & Birdsall, N.J.M. (1998). International Union of Pharmacology. XVII. Classification of muscarinic acetylcholine receptors. Pharmacol. Rev., 50, 279-290.
8. 50) of an enzymatic reaction. Biochem. Pharmacol., 22, 3099-3108.
9. Choppin, A., Eglen, R.M. & Hegde, S.S. (1998). Pharmacological characterization of muscarinic receptors in rabbit isolated iris sphincter muscle and urinary bladder smooth muscle. Br. J. Pharmacol., 124, 883-888.
10. Dǒrje, F., Wess, J., Lambrecht, G., Tacke, R., Mutschler, E. & Brann, M.R. (1991). Antagonist binding profiles of five cloned human muscarinic receptor subtypes. J. Pharmacol. Exp. Ther., 256, 727-733.
11. Eglen, R.M., Bonhaus, D.W., Calixto, J.J., Choppin, A., Leung, E., Loeb, M., Loury, D., Moy, T., Wilda, M. & Hegde, S.S. (1997). Characterization of the interaction of tolterodine at muscarinic receptor subtypes in vitro and in vivo. Br. J. Pharmacol., 120, 63P.
12. Ferraro-Dileo, G. & Flynn, D.D. (1995). Characterization of muscarinic receptors on cultured microglial cells. Life. Sci., 56, 1037 (abstract).
13. Gil, D.W., Krauss, H.A., Bogardus, A.M. & Woldemussie, E. (1997). Muscarinic receptor subtypes in human iris-ciliary body measurement by immunoprecipitation. Inv. Opthalmol. Vis. Sci., 38, 1434-1442.
14. 35S]-GTPγS binding mediated by human muscarinic m1-m4 receptors: antagonist studies. Br. J. Pharmacol., 109, 1120-1127.
15. Leff, P. & Dougall, I.G. (1993). Further concerns over Cheng-Prusoff analysis. Trends Pharmacol. Sci., 15, 141-144.
16. 5 (m5) receptor subtype: Fact or fiction? Life Sci., 60, 1105-1112.
17. Watson, N., Daniels, D.V., Ford, A.P.D.W., Eglen, R.M. & Hegde, S.S. (1998). Comparative pharmacology of human muscarinic m3 and m5 cholinoceptors expressed in chinese hamster ovary (CHO) cells. Br. J. Pharmacol., 123, 268P.
18. 5 receptors: Genotypes and Behaviour. Life Sci., abstract (in press).
19. Zalcman, S.J., Neckers, L.M., Kaayalp, O. & Wyatt, R.J. (1981). Muscarinic cholinergic binding sites on intact human lymphocytes. Life Sci., 29, 69-73.