Molecular evidence for cryptic species within Cylicostephanus minutus (Nematoda: Strongylidae)

International Journal for Parasitology

Volumn 29, Issue 2, 1999, Pages 285-291

  Hung, G C ^a   Chilton, N B ^a   Beveridge, I ^a   Zhu, X Q ^a   Lichtenfels, J R ^b   Gasser, R B ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Cryptic species; Cylistephanus minutus; First internal transcript spacer (ITS 1); Ribosomal DNA; Second internal transcript spacer (ITS 2); Single strand conformation polymorphism

Indexed keywords

RIBOSOME DNA; SPACER DNA;

ANIMAL EXPERIMENT; CONTROLLED STUDY; DNA POLYMORPHISM; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SHORT SURVEY; SINGLE STRAND CONFORMATION POLYMORPHISM; SPECIES DIFFERENCE; STRONGYLOIDEA; TAXONOMY;

CYLICOSTEPHANUS GOLDI; CYLICOSTEPHANUS LONGIBURSATUS; CYLICOSTEPHANUS MINUTUS; NEMATODA; STRONGYLIDAE;

EID: 0033035450     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0020-7519(98)00203-3     Document Type: Article

References (23)

1. Lichtenfels JR. Helminths of domestic equids. Proc Helminthol Soc Wash (special issue) 1975;42:1-92.
2. Lichtenfel JR, Kharchenko VA, Krecek RC, Gibbons LM. An annotated checklist, by genus and species level names for 51 recognized species of small strongyles (Nematoda: Strongyloidea: Cyathostominea) of horses, assess and zebras of the world. Vet Parasitol 1998;79:65-79.
3. Popova TI. Strongyloids of animals and man: Trichonematidae. In: Skrjabin KI, editor. Essentials of nematodology VII [English trans.]. Jerusalem: Israel Program for Scientific Translation, 1965. Original work published 1958.
4. Hartwich G. Zum Strongylus tetracanthus - Problem und zur Systematik der Cyathostominea (Nematoda: Strongyloidea). Mitteil Zool Mus Berlin 1986;62:61-102.
5. Lichtenfels JR, Kharchenko VA, Sommer C, Ito M. Key characters for the microscopical identification of Cylicocyclus nassatus and Cylicocyclus ashworthi (Nematoda, Cyathostominae) of the horse, Equus caballus. J Helminthol Soc Wash 1997;64:120-127.
6. Hung GC, Chilton NB, Beveridge I, et al. Molecular delineation of Cylicocyclus nassatus and C. ashworthi (Nematoda: Strongylidae). Int J Parasitol 1997;27:601-605.
7. Campbell AJD, Gasser RB, Chilton NB. Differences in a ribosomal DNA sequence of Strongylus species allows identification of single eggs. Int J Parasitol 1995;25:359-365.
8. Hoste H, Chilton NB, Gasser RB, Beveridge I. Differences in the second internal transcribed spacer (ribosomal DNA) between five species of Trichostrongylus (Nematoda, Trichoshrongylidae). Int J Parasitol 1995;25:75-80.
9. Romstad A, Gasser RB, Monti JR, et al. Differentiation of Oesophagostomum bifurcum from Necator americanus by PCR using genetic markers in spacer ribosomal DNA. Mol Cell Probes 1997;11:169-176.
10. Hoste H, Chilton NB, Beveridge I, Gasser RB. A comparison of the first internal transcribed spacer of ribosomal DNA in seven species of Trichostrongylus (Nematoda: Trichostrongylidae). Int J Parasitol 1998;28:1251-1260.
11. Newton LA, Chilton NB, Beveridge I, Gasser RB. Differences in the second internal transcribed spacer of four species of Nematodirus (Nematoda: Molineidae). Int J Parasitol 1998;28:337-341.
12. Chilton NB, Gasser RB, Beveridge I. Differences in a ribosomal DNA sequence of morphologically indistinguishable species within the Hypodontus macropi complex (Nematoda: Strongyloidea). Int J Parasitol 1995;25:647-651.
13. Chilton NB, Beveridge I, Andrews RH. Detection by allozyme electrophoresis of cryptic species of Hypodontus macropi (Nematoda: Strongyloidea) from macropodid marsupials. Int J Parasitol 1992;22:271-279.
14. Saiki RK, Gelfand DH, Stoffel S, et al. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 1988;239:487-491.
15. Gasser RB, Chilton NB, Hoste H, Beveridge I. Rapid sequencing of rDNA from single worms and eggs of parasitic helminths. Nucleic Acids Res 1993;21:2525-2526.
16. Sneath PHA, Sokal RR. Numerical taxonomy: the principles and practice of numerical classification. San Francisco: W.H. Freeman, 1973.
17. Gasser RB, Monti JR. Identification of parasitic nematodes by PCR-SSCP of ITS-2 rDNA. Mol Cell Probes 1997;11:201-209.
18. Cotton RGH. Mutation detection. Oxford: Oxford University Press, 1997.
19. Zhu XQ, Gasser RB. Single-strand conformation polymorphism (SSCP)-based mutation scanning approaches to fingerprint sequence variation in ribosomal DNA of ascaridoid nematodes. Electrophoresis 1998;19:1366-1373.
20. Bowles J, McManus DP. Rapid discrimination of Echinococcus species and strains using a polymerase chain reaction-based RFLP methods. Mol Biochem Parasitol 1993;57:231-240.
21. Gasser RB, Chilton NB. Characterisation of taeniid cestode species by PCR-RFLP of ITS-2 ribosomal DNA. Acta Trop 1995;59:31-40.
22. Gasser RB. Mutation scanning methods for the analysis of parasite genes. Int J Parasitol 1997;27:1449-1463.
23. Bøgh HO, Zhu XQ, Qian BZ, Gasser RB. Scanning for nucleotide variations in mitochondrial DNA fragments of Schistosoma japonicum by single-strand conformation polymorphism. Parasitology, in press.